“…Progress in molecular techniques produced a great impact on the knowledge of taxonomy, biology, pathogenesis, epidemiology and transmission of the agent and also on evaluation of cryptosporidiosis treatment, as well as helping epidemiologists regarding control and determination of infection sources 4,5,14,17,30 . Thus, among the molecular methods developed for the detection and differentiation of different Cryptosporidium species and genotypes, PCR is the mostly used for both clinical and environmental samples 8,11,28 , but its sensitivity is affected by the presence of inhibitors existing in fecal samples which may be minimized with changes in the extraction protocol 6,8,10,15,16,22,26 . In Brazil, most laboratories do not identify Cryptosporidium spp.…”