A atoxins are one of the major factors that affect the quality and safety of feeds. They can be transferred into livestock through contaminated feed and then onto humans via animal sources of food such as milk, meat and egg. The objective of this study was to detect and quantify the level of a atoxins (B1, B2, G1, G2, and total a atoxin) in dairy feeds, poultry (layer and broiler) feeds and feed ingredients produced in Addis Ababa. A total of 42 feeds and feed ingredients consisting of dairy feeds (n = 5), poultry broiler feeds (n = 6), layer feeds (n = 6), and feed ingredients (n = 25) were collected. The a atoxins were analyzed using high performance liquid chromatography after clean-up with an Immunoa nity columns. A atoxin B1 levels in feeds ranged from (51.66 to 370.51) µg/kg in dairy cattle feed; from (1.45 to 139.51) µg/kg in poultry layer feed; and from (16.49 to 148.86) µg/kg in broiler feed. A atoxin B1 levels in maize ranged from (2.64 to 46.74) µg/kg and in niger seed cake from (110.93 to 438.86) µg/kg.A atoxin B1 levels in wheat bran, wheat middling, and soybean were below 5 µg/kg. 100% of dairy feeds, 67% of poultry layer, 67% broiler feeds, and 24% of ingredients contained a atoxin in levels higher than the maximum tolerable limit set by the US Food and Drug Administration and Ethiopian Standard Agency. This shows the need for a strong regulatory monitoring and better feed management practices to prevent consumers of animal source foods from signi cant health impacts associated with a atoxins.