Incidences and antimicrobial susceptibility profile of Staphylococcus species isolated from animals in different Qatari farms

Abstract

This study was conducted during a six months period (March to August 2008) at the Veterinary laboratory, Department of Animal Resources, Doha, Qatar to determine the incidence of different bacterial genera farm animals in particular the occurrence of Staphylococcus species. The samples were from the rectum, milk, feces, nasal, swab of vagina, cloacal -uterin, intestine, liver and pus. Samples were collected from various animals of different regions. A total 318 of 429 samples gave positive results for Gram-negative and Gram-positive bacteria and 90 cultures were identified as Staphylococcus. The highest isolates were from camels' milk and none were obtained from specimens of cats and horses. A total of 33 selected Staphylococcus isolates were identified to species level and the most common isolated was coagulase-positive S. aureus (14), followed by coagulase-negative, S. lentus (10), S. xylosus (5), S. sciuri (2), S. lugdunensis (1) and S. caprae (1). The antimicrobial susceptibility profiles of these were determined using cephalothin, gentamycin, neomycin, ampicillin, norfloxacin, penicillin, tetracycline and a combination of trimethoprim and sulphamethoxazole. All identified species were sensitive to cephalothin except one strain of S. aureus. Most of the isolates showed multiple antimicrobial resistance profiles.

Antimicrobial Resistance Profiles of Bacteria Isolated from the Nasal Cavity of Camels in Samburu, Nakuru, and Isiolo Counties of Kenya

“…This was also reported in other studies [42, 43]. Bhatt et al [44] reported isolation of high proportion of multidrug-resistant Staphylococcus organisms from surgical wounds.…”

“…The susceptibility of coagulase-negative Staphylococcus in this study was least in Ampicillin and Tetracycline; a study by Al-Thani and Al-Ali [42] reported Staphylococcus species to be resistant to Tetracycline, Penicillin, and Ampicillin in different Qatari farms. However, the same study reported the organisms being very susceptible to Cephalothin, Norfloxacin, and Co-trimoxazole.…”

Antibiotic Susceptibility Profile of Methicillin Resistant Staphylococci aureus in Poultry Farm, in Zaria, Nigeria

“…The isolates showed high resistance (74.7% and 50.4%) to Tetracycline and Ampicillin respectively while 41.2% of the isolates were resistant to Methicillin and produced β-lactamase enzyme ( Figure 1). The observed variation in the percentage susceptibility profile expressed by S. aureus in this study also concurred with the report of Roda and Fatema [22] who observed a variation of 3 to 66.66%. The study of Roda and Fatema [22] and the work of Yaqub et al, [23,24] also observed high resistance to penicillin and tetracycline.…”

“…The observed variation in the percentage susceptibility profile expressed by S. aureus in this study also concurred with the report of Roda and Fatema [22] who observed a variation of 3 to 66.66%. The study of Roda and Fatema [22] and the work of Yaqub et al, [23,24] also observed high resistance to penicillin and tetracycline. This could be associated with the vast used of these antibiotics for the treatment of animal diseases [25].…”

“…The most commonly isolated of this specie is coagulase positive (89.8% (88)) while coagulase negative were 10.2% (10) ( Table 1). The report of Roda and Fatema [22] concurred with the high percentages of coagulase positive S. aureus (42.4% (14)) in poultry farms compared with the coagulase negative (30.3%) S. aureus. The susceptibility profile of the S. aureus to commonly used antibiotics in the hospital and poultry management showed that the isolates were highly susceptible to Ciprofloxacin, Vancomycin, Pefloxacin and Gentamycin.…”