Incidence, virulence genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood

Stratev, Deyan; Fasulkova, Rumyana; Krumova-Valcheva, Gergana

doi:10.1016/j.micpath.2023.106050

Cited by 12 publications

(15 citation statements)

References 34 publications

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“…However, 2 isolates showed intermediate resistance to cefotaxime, and 3 isolates exhibited intermediate resistance to chloramphenicol. These findings are consistent with those of previous studies [ 12 , 13 , 39 , 41 ], which reported that over 90% of V. parahaemolyticus strains were susceptible to these antibiotics. Therefore, these drugs are considered the most effective antimicrobials for treating V. parahaemolyticus infections in the areas studied.…”

Section: Discussionsupporting

confidence: 93%

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Nguyen,

Truong,

Thi

et al. 2024

PHRP

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Objectives: Vibrio parahaemolyticus is a major foodborne pathogen in aquatic animals and a threat to human health worldwide. This study investigated the prevalence, antimicrobial resistance, antimicrobial resistance genes (ARGs), and biofilm formation of V. parahaemolyticus strains isolated from fish mariculture environments in Cat Ba Island, Vietnam.Methods: In total, 150 rearing water samples were collected from 10 fish mariculture farms in winter and summer. A polymerase chain reaction assay was used to identify V. parahaemolyticus, its virulence factors, and ARGs. The antimicrobial resistance patterns and biofilm formation ability of V. parahaemolyticus strains were investigated using the disk diffusion test and a microtiter plate-based crystal violet method, respectively.Results: Thirty-seven V. parahaemolyticus isolates were recovered from 150 samples. The frequencies of the tdh and trh genes among V. parahaemolyticus isolates were 8.1% and 21.6%, respectively. More than 90% of isolates were susceptible to ceftazidime, cefotaxime, and chloramphenicol, but over 72% were resistant to ampicillin, tetracycline, and erythromycin. Furthermore, 67.57% of isolates exhibited multidrug resistance. The presence of ARGs related to gentamicin (aac(3)-IV), tetracycline (tetA) and ciprofloxacin (qnrA) in V. parahaemolyticus isolates was identified. Conversely, no ARGs related to ampicillin or erythromycin resistance were detected. Biofilm formation capacity was detected in significantly more multidrug-resistant isolates (64.9%) than non-multidrug-resistant isolates (18.9%).Conclusion: Mariculture environments are a potential source of antibiotic-resistant V. parahaemolyticus and a hotspot for virulence genes and ARGs diffusing to aquatic environments. Thus, the prevention of antibiotic-resistant foodborne vibriosis in aquatic animals and humans requires continuous monitoring.

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Section: Discussionsupporting

confidence: 93%

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Nguyen,

Truong,

Thi

et al. 2024

PHRP

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“…This is again reflected in phenotypical studies on V. parahaemolyticus clinical and environmental strains, where low prevalence of resistance towards tetracyclines [ e.g,. 0.3% to doxycycline [ 30 , 31 , 36 ], 0–15% to tetracycline [ 27 , 28 , 30 – 33 , 35 , 36 ]], trimethoprim-sulfamethoxazole [0–7% [ 27 , 30 , 31 , 33 ]], and fluoroquinolones ( e.g,. 0% to ciprofloxacin [ 27 , 30 ] and 0–1% to levofloxacin [ 27 , 33 ]] were reported.…”

Section: Discussionmentioning

confidence: 99%

“…0.3% to doxycycline [ 30 , 31 , 36 ], 0–15% to tetracycline [ 27 , 28 , 30 – 33 , 35 , 36 ]], trimethoprim-sulfamethoxazole [0–7% [ 27 , 30 , 31 , 33 ]], and fluoroquinolones ( e.g,. 0% to ciprofloxacin [ 27 , 30 ] and 0–1% to levofloxacin [ 27 , 33 ]] were reported. However, reported resistance prevalence against aminoglycosides is very variable [ e.g,.…”

Section: Discussionmentioning

confidence: 99%

“…This, in turn, has led to increased antibiotic usage among farms worldwide [ 26 ]. However, the improper, empirical, and extensive application of antibiotics has resulted in the emergence of antimicrobial resistance in a substantial amount of V. parahaemolyticus strains in the environment [ 27 – 29 ]. Every year, there is a reported increase in the multidrug resistance of pathogenic V. parahaemolyticus to clinically important antimicrobial drugs.…”

Section: Introductionmentioning

confidence: 99%

“…Every year, there is a reported increase in the multidrug resistance of pathogenic V. parahaemolyticus to clinically important antimicrobial drugs. This not only reduces the effectiveness of antibiotics used in aquaculture practices but also poses a threat to human health due to the transmission of V. parahaemolyticus strains carrying (mobilizable) antimicrobial resistance genes (ARGs) to humans upon consumption [ 27 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Genomic mining of Vibrio parahaemolyticus highlights prevalence of antimicrobial resistance genes and new genetic markers associated with AHPND and tdh + /trh + genotypes

Vandeputte,

Coppens,

Bossier

et al. 2024

BMC Genomics

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Background Acute Hepatopancreatic Necrosis Disease (AHPND) causes significant mortality in shrimp aquaculture. The infection is primarily instigated by Vibrio parahaemolyticus (Vp) strains carrying a plasmid encoding the binary toxin PirAB. Yet, comprehension of supplementary virulence factors associated with this relatively recent disease remains limited. Furthermore, the same holds for gastroenteritis in humans caused by other Vp genotypes. Additionally, given the prevalent use of antibiotics to combat bacterial infections, it becomes imperative to illuminate the presence of antimicrobial resistance genes within these bacteria. Results A subsampled number of 1,036 Vp genomes was screened for the presence of antimicrobial resistance genes, revealing an average prevalence of 5 ± 2 (SD) genes. Additional phenotypic antimicrobial susceptibility testing of three Vp strains (M0904, TW01, and PV1) sequenced in this study demonstrated resistance to ampicillin by all tested strains. Additionally, Vp M0904 showed multidrug resistance (against ampicillin, tetracycline, and trimethoprim-sulfamethoxazole). With a focus on AHPND, a screening of all Vibrio spp. for the presence of pirA and/or pirB indicates an estimated prevalence of 0.6%, including four V. campbellii, four V. owensii, and a Vibrio sp. next to Vp. Their pirAB-encoding plasmids exhibited a highly conserved backbone, with variations primarily in the region of the Tn3 family transposase. Furthermore, an assessment of the subsampled Vp genomes for the presence of known virulence factors showed a correlation between the presence of the Type 3 Secretion System 2 and tdh, while the presence of the Type 6 Secretion System 1 was clade dependent. Furthermore, a genome-wide association study (GWAS) unveiled (new) genes associated with pirA, pirB, tdh, and trh genotypes. Notable associations with the pirAB genotype included outer membrane proteins, immunoglobulin-like domain containing proteins, and toxin-antitoxin systems. For the tdh + /trh + genotypes (containing tdh, trh, or both genes), associations were found with T3SS2 genes, urease-related genes and nickel-transport system genes, and genes involved in a ‘minimal’ type I-F CRISPR mechanism. Conclusions This study highlights the prevalence of antimicrobial resistance and virulence genes in Vp, identifying novel genetic markers associated with AHPND and tdh + /trh + genotypes. These findings contribute valuable insights into the genomic basis of these genotypes, with implications for shrimp aquaculture and food safety.

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Molluscan shellfish: a potential source of pathogenic and multidrug-resistant Vibrio spp.

Dahanayake

Majeed

Kumarage

et al. 2023

J Consum Prot Food Saf

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Incidence, virulence genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood

Cited by 12 publications

References 34 publications

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Genomic mining of Vibrio parahaemolyticus highlights prevalence of antimicrobial resistance genes and new genetic markers associated with AHPND and tdh + /trh + genotypes

Molluscan shellfish: a potential source of pathogenic and multidrug-resistant Vibrio spp.

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