Incidence of Zika Virus Infection from a Dengue Epidemiological Study of Children in Ratchaburi Province, Thailand

Sriburin, Pimolpachr; Sittikul, Pichamon; Kosoltanapiwat, Nathamon; Sirinam, Salin; Arunsodsai, Watcharee; Sirivichayakul, Chukiat; Limkittikul, Kriengsak; Chatchen, Supawat

doi:10.3390/v13091802

Cited by 7 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, our group reported using ZIKV NS1 IgG ELISA screening to identify symptomatic ZIKV infections [ 21 ]. However, only the sample with a high P/N ratio (>10) showed a positive score with the ZIKV NS1 BOB ELISA, as shown in Figure 2 A,B.…”

Section: Resultsmentioning

confidence: 99%

“…All 30 ZIKV-positive samples (1–2 weeks after symptoms onset) were selected from a study of Zika incidence in dengue epidemiology study in Ratchaburi province, Thailand [ 20 , 21 ]. All samples showed seropositivity using ZIKV NS1 IgG ELISA, and ZIKV infection was confirmed by RT-PCR in acute sera [ 21 ]. All procedures were ethically approved before the study (TMEC 21-013).…”

Section: Methodsmentioning

confidence: 99%

“…Indirect ELISA was performed using a previously described standard protocol [ 21 , 22 ]. In brief, plates were sensitized with ZIKV NS1 (10 µg/mL or 500 ng/well), DENV 1–4 NS1 (5 µg/mL, 250 ng for each serotype or a total of 1000 ng/well), or inactivated ZIKV (MR766) in PBS buffer and incubated at 37 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas

et al. 2022

View full text Add to dashboard Cite

Zika virus (ZIKV) is a mosquito-borne flavivirus that has recently emerged as a global health threat. The rise in ZIKV infections has driven an increased incidence of neonates born with microcephaly or other neurological malformations. Therefore, screening for ZIKV infection can considerably impact pregnant women, especially during the first trimester. The majority of ZIKV infections are mild or asymptomatic, and clinical diagnosis is inaccurate. Moreover, given the high level of cross-reactivity among flaviviruses, serological approaches to distinguish ZIKV from dengue virus (DENV) infections are complicated. We used the combination of DENV and ZIKV nonstructural protein 1 (NS1) IgG enzyme-linked immunosorbent assay (ELISA) and ZIKV NS1 blockade-of-binding (BOB) ELISA to test the convalescent sera of non-flavivirus, primary DENV, secondary DENV, and ZIKV infections. Our findings indicate that primary testing using a ZIKV NS1 IgG ELISA, the test of choice for large-scale ZIKV serosurvey studies, provided relatively high sensitivity. Moreover, the confirmation of positive ELISA results using the ZIKV NS1 BOB ELISA increased average specificity to 94.59% across serum samples. The combined use of two simple ELISAs for ZIKV serosurveys and the monitoring of ZIKV infection during pregnancy can elucidate the epidemiology, pathogenesis, and complications of ZIKV in DENV-endemic areas.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Interestingly, although the four countries are in Southeast Asia and South America, they are all located within the region 30° south to 30° north latitude. All four countries have tropical or subtropical rainforest climates, with high temperatures and high humidity throughout the year, and this environment is conducive to the spread and mutation of the virus ( Hamel et al, 2021 ; Hattakam et al, 2021 ; Munoz et al, 2021 ; Ngim et al, 2021 ; Sriburin et al, 2021 ). In subtropical rainforests, PCV3 is not the only prevalent virus; other viruses such as the Zika virus and dengue virus are also prevalent, among others ( Hamel et al, 2021 ; Hattakam et al, 2021 ; Munoz et al, 2021 ; Ngim et al, 2021 ; Sriburin et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of the Evolutionary Origin, Phylodynamics, and Phylogeography of the Porcine Circovirus Type 3

et al. 2022

View full text Add to dashboard Cite

Porcine circovirus type 3 (PCV3) is a newly identified virus associated with porcine dermatitis and nephropathy syndrome (PDNS) and multisystemic inflammatory responses in pigs. Recent studies suggests that PCV3 originated from bat circoviruses; however, the origin time, mode of spread, and geographic distribution of PCV3 remain unclear. In this study, the evolutionary origin, phylodynamics, and phylogeography of PCV3 were reconstructed based on the available complete genome sequences. PCV3 showed a closer relationship with bird circovirus than with bat circovirus, but their common ancestor was bat circovirus, indicating that birds may be intermediate hosts for the spread of circoviruses in pigs. Using the BEAST and phylogenetic analyses, three different clades of PCV3 (PCV3a, PCV3b, and PCV3c) were identified, with PCV3a being the most prevalent PCV3 clade. Further studies indicated that the earliest origin of PCV3 can be traced back to 1907.53–1923.44, with a substitution rate of 3.104 × 10–4 to 6.8524 × 10–4 substitution/site/year. A phylogeographic analysis highlighted Malaysia as the earliest location of the original PCV3, which migrated to Asia, America, and Europe. Overall, this study provides novel insights into the evolutionary origin, spread mode, and geographic distribution of PCV3, which will facilitate the prevention and control of PCV3 epidemics in the future.

show abstract

“…For ZIKV RT-PCR, urine samples from the participants with acute febrile illness were selected for RNA extraction using a QIAGEN Viral RNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The processes were performed as previously described [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Seroprevalence of Zika Virus in Amphawa District, Thailand, after the 2016 Pandemic

Sirinam

Chatchen

Arunsodsai

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

In 2016, Zika virus (ZIKV) infection was declared a public health emergency of international concern because of the neurological consequences in babies born to infected people. Because of the mild and nonspecific symptoms, serological tests are essential in epidemiological studies. However, cross-reactive antibodies between other Flaviviridae members may complicate the interpretation of results of these tests. This study investigated the seroprevalence of ZIKV infection in Samut Songkhram in central Thailand which was affected by the Zika outbreak of 2016. Three hundred and fifty volunteers aged 5–50 years in Amphawa District, Samut Songkhram, were enrolled between April 2017 and April 2018. ZIKV nonstructural protein 1 (NS1) immunoglobulin G enzyme-linked immunosorbent assay (ELISA) was used to screen serum samples collected on the first day of enrollment and after 6 and 12 months. The seroprevalence and seroconversion of ZIKV were assessed. Cases of ZIKV seroconversion were verified as evidence of ZIKV infection by NS1 blockade-of-binding ELISA and plaque reduction neutralization test (PRNT50). ZIKV seroprevalence in Amphawa was 15.1–17.8% with no significant change over the year. The total seroconversion rate throughout the year was 7/100 person-years. The ratio of asymptomatic to symptomatic infections was 4.5:1. The cases in our study confirmed the occurrence of occult ZIKV infections in the community. These undetected infections might promote the spread of ZIKV in vulnerable groups of the community.

show abstract

Incidence of Zika Virus Infection from a Dengue Epidemiological Study of Children in Ratchaburi Province, Thailand

Cited by 7 publications

References 26 publications

Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas

Combining Immunoassays to Identify Zika Virus Infection in Dengue-Endemic Areas

Reconstruction of the Evolutionary Origin, Phylodynamics, and Phylogeography of the Porcine Circovirus Type 3

Seroprevalence of Zika Virus in Amphawa District, Thailand, after the 2016 Pandemic

Contact Info

Product

Resources

About