Incidence of water-soaking and phospholipid catabolism in ripe watermelon (Citrullus lanatus) fruit: induction by ethylene and prophylactic effects of 1-methylcyclopropene

Mao, Linchun; Karakurt, Yaşar; Huber, Donald J.

doi:10.1016/j.postharvbio.2003.12.007

Cited by 64 publications

(43 citation statements)

References 35 publications

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“…Studies with immature and ripe watermelon fruits exposed to ethylene reported that fruit cell walls showed extensive solubilization and depolymerization, and the activities and transcript levels of cell wall-degrading enzymes, including polygalacturonase, expansin, and galactosidases, and those of membrane-degrading enzymes including lipoxygenase and phospholipases demonstrated significant increases with ethylene exposure Huber, 2002, 2004). Exposure of watermelon fruit to ethylene has also caused extensive degradation in phospholipids (Mao et al, 2004). Similar results have also been reported for cucumber fruit (Lima et al, 2005).…”

Section: Introductionsupporting

confidence: 81%

“…Samples were taken from the placental tissue and frozen in liquid nitrogen. Some of the fruits were also treated with 10 µL/L 1-methylcyclopropene (1-MCP) as previously described (Mao et al, 2004). Moreover, fruit tissue samples were also obtained during the developmental period (15-75 days after full flowering) and frozen in liquid nitrogen.…”

Section: Plant Materials and Rna Isolationmentioning

confidence: 99%

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The molecular characterization and expression analyses of ethylene receptor genes from watermelon fruit

Karakurt¹,

Tonguç²,

Ünlü³

2014

Turk J Bot

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Watermelon fruit is very sensitive to ethylene, showing rapid deterioration and water-soaking upon ethylene exposure. The objective of this study was to isolate and determine the expressions of ethylene receptor genes in watermelon fruit. As a result of PCR amplification of cDNAs obtained via RT-PCR of total RNA from watermelon fruit with degenerate primers, 3 partial cDNAs were obtained. After extension of the 3' and 5' ends of the cDNAs via 3' and 5' RACE analysis, 3 full-length genes designated as CL-ETR1, CL-ERS1, and CL-ETR2 were obtained. Upon cloning and sequencing, it was determined that the CL-ETR1, CL-ERS1, and CL-ETR2 genes were 2343, 2349, and 2550 bp in length, respectively. The structure and sequence analysis showed that the isolated genes had conserved domains found in previously isolated ETR genes. Southern blot analysis showed a single band for CL-ETR1 and CL-ETR2 and 2 bands for CL-ERS1. Northern blot analyses showed that the expression levels of all 3 receptor genes significantly increased during postharvest storage and in response to ethylene exposure. 1-Methylcyclopropene (1-MCP) treatment significantly reduced the expressions of all receptor genes. The genes also showed different expression patterns during fruit development. The isolated genes could be used in biotechnological studies to develop cultivars with altered ripening behavior that are more tolerant to ethylene-related physiological disorders such as water-soaking.

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Section: Introductionsupporting

confidence: 81%

Section: Plant Materials and Rna Isolationmentioning

confidence: 99%

The molecular characterization and expression analyses of ethylene receptor genes from watermelon fruit

Karakurt¹,

Tonguç²,

Ünlü³

2014

Turk J Bot

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“…Firmness is the measured parameter related to softening; in 1-MCP treated "d´Anjou" pear, firmness was higher than in nontreated fruits (Argenta et al, 2003). Better firmness was also observed in several 1-MCP treated fruits, including avocado (Feng et al, 2000), strawberry (Jiang et al, 2001), guavas (Bassetto et al, 2005), plum cultivars (Martinez-Romero et al, 2003), pear (Hiwasa et al, 2003;Ekman et al, 2004), watermelon (Mao et al, 2004), banana (Lohani et al, 2004), kiwifruit (Boquete et al, 2004), apple (Defilippi et al, 2004) and nectarine (Bregoli et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Novel Postharvest Treatment Reduces Ascorbic Acid Losses in Mango (<i>Mangifera indica</i> L.) Var. Kent

Islas-Osuna¹

2010

American Journal of Agricultural and Biological Sciences

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Problem statement:Mango is a tropical fruit that ripens very quickly; for this reason, there has been a continuous effort to develop postharvest technologies to extend its shelf life and quality. Among them, 1-Methylcyclopropene (1-MCP) is widely used because it inhibits the action of ethylene receptors. Approach: Changes in physicochemical parameters, bioactive compounds and cell wall degrading activities were evaluated during storage and ripening of fresh whole mangoes treated with 1-MCP (750 nL L −1 ). Mature-green mangoes, cultivar Kent, untreated or treated with 1-MCP were evaluated for external quality, phytochemicals, Polygalacturonase (PG) and Pectin Methylesterase (PME) enzymatic activities during storage at 20°C for 2 weeks. Results: Concentration of ascorbic acid decreased during fruit ripening but 1-MCP-treated mangoes had reduced losses. Polygalacturonase and pectin methylesterase activities were reduced in the treated fruits as compared to untreated mangoes. Small changes in β-carotene were observed between treated and untreated fruits. Conclusion: 1-MCP affected the ripening process in "Kent" mango, reducing losses of ascorbic acid, this treatment is justified since it helps to maintain mango's nutritional value during its shelf life.

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“…16,17 Other responses include enhanced activity of hydrolytic enzymes targeting both cell walls and membranes, and include increases in transcript abundance for proteins including lipoxygenase (LOX, EC 1.13.11.12), expansin, polygalacturonase (PG, EC 3.2.1.15) and phospholipases C (PLC, EC 3.1.4.3) and D (PLD, EC 3.1.4.4) 18 and increased phospholipid catabolism. 19 The susceptibility of watermelon fruit to ethyleneinduced damage is maturity independent, with both immature and full ripe fruit exhibiting similar responses to the gas. 17 If watermelon placental tissue exhibits a significant ethylene production burst in response to fresh-cut processing, then this might contribute to the juice leakage and water soaking noted for fresh-cut watermelon, 14 representing an adverse response paralleling that characteristic of intact fruit exposed to ethylene.…”

Section: Introductionmentioning

confidence: 99%

Physiological properties of fresh‐cut watermelon (Citrullus lanatus) in response to 1‐methylcyclopropene and post‐processing calcium application

et al. 2005

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Seedless watermelon (Citrullus lanatus Thunb. Mansfeld, cv. Millionaire) fruit were exposed to 10 µL L −1 1-methylcyclopropene (1-MCP) or air for 18 h. Afterward, the fruit were processed into placental-tissue pieces, treated with calcium dips (20 g kg −1 CaCl 2 ) or deionized water, and stored in vented containers for 7 days at 10 • C. At intervals during storage, fresh-cut placental tissue was monitored for respiration, ethylene production, firmness, electrolyte leakage, total soluble solids, titratable acidity and microbial growth. Ethylene production was below detection in fresh-cut placental tissue, consistent with the low ethylene production in intact watermelon fruit. Respiration rates were significantly enhanced in response to tissue processing, and continued to increase throughout the 7 days of storage. Tissue derived from 1-MCP-treated fruit showed enhanced 1-aminocyclopropane-1-carboxylate synthase (ACS, EC 4.4.1.14) activity, suppressed respiratory rates and undetectable levels of 1-aminocyclopropane-1-carboxylate oxidase (ACO) activity during storage. Post-processing calcium dips (CaCl 2 ) had little influence on ACS activity relative to tissue not receiving calcium, but significantly enhanced ACO activity and maintained firmness of fresh-cut tissue throughout storage. The data collectively support the conclusion that 1-methylcyclopropene treatment of intact watermelon fruit is alone unlikely to benefit the storage duration of fresh-cut watermelon.

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Incidence of water-soaking and phospholipid catabolism in ripe watermelon (Citrullus lanatus) fruit: induction by ethylene and prophylactic effects of 1-methylcyclopropene

Cited by 64 publications

References 35 publications

The molecular characterization and expression analyses of ethylene receptor genes from watermelon fruit

The molecular characterization and expression analyses of ethylene receptor genes from watermelon fruit

Novel Postharvest Treatment Reduces Ascorbic Acid Losses in Mango (<i>Mangifera indica</i> L.) Var. Kent

Physiological properties of fresh‐cut watermelon (Citrullus lanatus) in response to 1‐methylcyclopropene and post‐processing calcium application

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