Abstract:Background: The epidemiology of inflammatory bowel disease (IBD) is changing globally and there is disagreement between studies about the prevalence of relapse of ulcerative colitis (UC) as a type of IBD.
Ulcerative colitis (UC) and Crohn's disease (CD), classified as inflammatory bowel diseases (IBD), are lifelong diseases, arise from an interaction between genetic and environmental factors, and are predominantly observed in developed countries (Hosseini et al, 2016). They are increasing worldwide with the increasing pace of the westernization of societies.
Ulcerative colitis (UC) and Crohn's disease (CD), classified as inflammatory bowel diseases (IBD), are lifelong diseases, arise from an interaction between genetic and environmental factors, and are predominantly observed in developed countries (Hosseini et al, 2016). They are increasing worldwide with the increasing pace of the westernization of societies.
“…Inflammatory bowel disease (IBD) consists of two similar but distinct diseases including ulcerative colitis (UC) and Crohn's disease (CD) (Hosseini et al 2016). The etiology of UC is not clearly known and some different reasons such as immune system dysfunction, genetic susceptibility, changes in the gut flora, and environmental factors may be involved Tanideh et al 2017).…”
“…Changing of lifestyle increases the prevalence of multifactorial and chronic diseases, such as inflammatory bowel disease (IBD), in different manners. 1 IBD is characterized by idiopathic inflammation of the gastrointestinal (GI) tract and has 2 main types, ulcerative colitis (UC) and Crohn' s disease (CD). 2,3 UC is a costly disease and imposes a heavy direct and indirect burden on populations.…”
Background/Aims: Ulcerative colitis (UC), along with Crohn’s disease, is one of the main types of inflammatory bowel disease (IBD). On the other hand, deregulated autophagy is involved in many chronic diseases, including IBD. In this study, we aimed to investigate the role of Atg5 and microRNA-181a (miR-181a) in the pathophysiology of UC. Methods: Colon biopsy, stool, and blood samples of 6 men and 9 women were confirmed for UC. Also, 13 men and 17 women were selected as healthy control (HC). Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to measure the Atg-5 content of the colon biopsies. Besides, the serum and stool levels of Atg5 were measured using ELISA. Moreover, the total RNA of blood cells was extracted and evaluated for the expression of miR-181a.Results: We found 1.2 ng/mL versus 0.46 ng/mL, 0.34 ng/mL versus 0.24 ng/mL, and 0.082 ng/mL versus 0.062 ng/mL of Atg5 in stool, intestinal tissue, and serum of UC and HCs, respectively. There was no significant difference in the expression of miR-181a in the blood samples of UC and HCs. Immunohistochemistry showed high positivity without any significant difference between the 2 groups in the quantitative analysis.Conclusions: The significant difference observed between the stool Atg5 content of the HCs and UC patients may provide new insight into using this protein as a diagnostic biomarker, however, considering the small size of our studied population further studies are needed.
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