Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia

Župunski, Vesna; Jevtić, Radivoje; Ignjatović-Micić, Dragana; Stankоvić, Slavica; Lević, J.; Ivanović, Dragica; Nikolić, Ana

doi:10.15258/sst.2012.40.3.04

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…While analyses based on ITS, EF1a and RPB2 showed variation among sequences for DB compared to CB, support was lacking and sampling was limited (Carris et al 2007). Importantly, T. controversa can cause major yield losses and is an internationally quarantined pathogen to prevent its entry to new areas (OEPP/EPPO 2016) and CB, which has been successfully controlled with chemical seed treatments for many years, has made a resurgence under lowinput and organic production (Borgen and Davanlou 2001;Matanguihan et al 2011;Župunski et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Genome sequencing and comparison of five Tilletia species to identify candidate genes for the detection of regulated species infecting wheat

et al. 2019

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Tilletia species cause diseases on grass hosts with some causing bunt diseases on wheat (Triticum). Two of the four species infecting wheat have restricted distributions globally and are subject to quarantine regulations to prevent their spread to new areas. Tilletia indica causes Karnal bunt and is regulated by many countries while the nonregulated T. walkeri is morphologically similar and very closely related phylogenetically, but infects ryegrass (Lolium) and not wheat. Tilletia controversa causes dwarf bunt of wheat (DB) and is also regulated by some countries, while the closely related but non-regulated species, T. caries and T. laevis, both cause common bunt of wheat (CB). Historically, diagnostic methods have relied on cryptic morphology to differentiate these species in subsamples from grain shipments. Of the DNA-based methods published so far, most have focused on sequence variation among tested strains at a single gene locus. To facilitate the development of additional molecular assays for diagnostics, we generated whole genome data for multiple strains of the two regulated wheat pathogens and their closest relatives. Depending on the species, the genomes were assembled into 907 to 4633 scaffolds ranging from 24 Mb to 30 Mb with 7842 to 9952 gene models predicted. Phylogenomic analyses confirmed the placement of Tilletia in the Exobasidiomycetes and showed that T. indica and T. walkeri were in one clade whereas T. controversa, T. caries and T. laevis grouped in a separate clade. Single copy and species-specific genes were identified by orthologous group analysis. Unique species-specific genes were identified and evaluated as suitable markers to differentiate the quarantine and non-quarantine species. After further analyses and manual inspection, primers and probes for the optimum candidate genes were designed and tested in silico, for validation in future wet-lab studies.

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Section: Introductionmentioning

confidence: 99%

Genome sequencing and comparison of five Tilletia species to identify candidate genes for the detection of regulated species infecting wheat

et al. 2019

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“…could also be a good candidate, but its teliospores are larger [ca. 16-24 µm (Trione 1974;Pimentel et al 2000;Župunski et al 2012)] so this would be di cult unless the specimen photographed was quite immature, which is not probable. It is rather unlikely that this last type of spores belongs to Puccinia Pers., a genus that commonly infects wheat, causing rust.…”

Section: Fungal Sporesmentioning

confidence: 99%

Identification of Neolithic diet by the morphology of the starch grains of dental calculus found in the Dehesilla Cave (Cadiz – South of the Iberian Peninsula)

Coutinho

Cardoso-Moreira

Silva

et al. 2023

Preprint

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The starch grains present in the dental calculus of four Neolithic individuals of the Dehesilla Cave (Cadiz, South of Spain) were extracted and observed with light microscopy. The variant of the extraction technique used proved to be very effective. A number of Poaceae-Triticeae taxa [wheat (Triticum sp., the main crop, including Triticum aestivum), barley (Hordeum sp.)], Fabaceae-Fabeae-Cicereae and Fagaceae (oaks = Quercus sp., which constitute new data for the area in question.) were identified. Some pollen grains and fungal spores were also identified, which helped to provide information on the ecological framework of the crops associated with the archaeological site.

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“…Âîíà çóñòð³÷àºòüñÿ â áàãàòüîõ êðà¿íàõ ñâ³òó (ðèñ. 2), çîêðåìà â ÑØÀ, Êàíàä³, Í³ìå÷÷èí³, Øâåé-öàð³¿, ²òàë³¿, Àâñòð³¿, Øâåö³¿, ×åõ³¿, ²ðàí³, Ðîñ³¿, Ëàòâ³¿, Ñåðá³¿, Êàçàõ-ñòàí³ [7][8][9][10][11].…”

Section: ¹10-12 (263) 2020unclassified

“…Òåìïåðàòóðè âèùå 15°Ñ ïðèãí³÷óþòü ïðîðîñòàííÿ ñïîð [6]. [7]. Ó ×åõ³¿ â êîìïëåêñ³ ñàaeêîâèõ õâîðîá, ïîðÿä ç³ çðîñòàííÿì çàãàëüíîãî ð³âíÿ óðàaeåííÿ, â³äçíà÷åíî âèùèé ð³âåíü ³íô³êóâàííÿ T. controversa [9].…”

Section: ¹10-12 (263) 2020mentioning

confidence: 99%

Tilletia species on winter wheat grain

Shevchuk¹,

Kyslykh²,

Holosna³

et al. 2020

qpp

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Goal. To determine the species composition of the complex of pathogens of bunt diseases of the genus Tilletia on winter wheat grain. Methods. The research was conducted during 2017—2020. 315 samples of winter wheat grain from different soil and climatic zones were analyzed. Seed contamination was determined by washing the grains and centrifuging the spore suspension, followed by counting their number in the Goryaev chamber. Identification of the pathogen was performed in the laboratory by microscopic examination, taking into account the morphological features of teliospores. Results. In 2017 and 2018, the presence of spores of fungi of the genus Tilletia was detected in 23.5 and 28.6% of the analyzed wheat grain samples, respectively. The number of spores per grain varied between 11—388.9 and 2.8—214.8 and averaged 72.5 and 33.7 correspondingly. The largest number of infected samples was observed in 2019 — 47.4%. In the same period, the highest level of seed contamination was observed — up to 1089 spores per grain, in average — 124.6 spores per grain. The lowest level of grain contamination was detected in 2020 — 6.3% of samples with an average of 3.7 spores per grain. Mostly spores of bunt pathogens were found in grain samples from Ternopil, Kyiv, Chernihiv, Lviv, Vinnytsia regions. The following species have been identified: Tilletia caries, T. controversa, T. laevis. Pathogen T. caries was detected annually. It dominated in 2020 and ranked second in detection rate in 2017—2019. T. controversa was found on grain in 2017—2019 and prevailed among other species. Its part in the complex of pathogens varied from 77.1 to 87.5%. In 2018, T. laevis was also detected. Conclusions. In most samples, spores of fungi of the genus Tilletia were not detected. On average, according to the years of research, grain contamination by bunt fungi were detected in 6.3—47.4% of samples with an average spore load of 3.7—124.6 of grain. The highest number of infected samples and the level of contamination were observed in 2019, the lowest — in 2020. The complex of pathogens is represented by three species: T. caries, T. laevis, T. controversa. T. controversa dominated in 2017—2019 and T. caries — in 2020. T. laevis occurred sporadically.

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Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia

Cited by 6 publications

References 19 publications

Genome sequencing and comparison of five Tilletia species to identify candidate genes for the detection of regulated species infecting wheat

Genome sequencing and comparison of five Tilletia species to identify candidate genes for the detection of regulated species infecting wheat

Identification of Neolithic diet by the morphology of the starch grains of dental calculus found in the Dehesilla Cave (Cadiz – South of the Iberian Peninsula)

Tilletia species on winter wheat grain

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