Incidence of Motile Aeromonas Species in Aquatic Environments of Rio De Janeiro; Brazil

Abstract

Fresh and salt water samples collected in Rio de Janeiro city were analysed for the presence of motile Aeromonas species. Twenty-six out of 50 aliquots analysed (52%) were positive for Aeromonas. One hundred strains were isolated from both environments (A. caviae, 60%; A. veronii, 14%; A. hydrophila, 1%; A. sobria, 1%; and Aeromonas sp., 24%). Minimal tests such as oxidase, motility, sensitivity to the vibriostatic agent 2,4-diamino-6,7 - diisopropylpteridine, fermentation and gas from glucose, acetoin from glucose (Voges-Proskauer), lysine decarboxylase, ornithine decarboxylase, and esculin hydrolysis were sufficient to classify the majority Aeromonas strains into species. No Aeromonas was found in nonpolluted waters but, in contrast, both fresh and salt polluted waters showed a high incidence of isolates. Most of the Aeromonas strains analysed produced hemolysin and/or heat-stable enterotoxin. The latter was produced by 73% of the A. veronii isolates.

Prevalence and virulence properties of Vibrio cholerae non-O1, Aeromonas spp. and Plesiomonas shigelloides isolated from Cambe Stream (State of Parana, Brazil)

“…One sediment was resuspended in 10 ml peptone water, pH 7Á2, and incubated at 37 C for 24 h for the enrichment of Aeromonas and Plesiomonas (Neves et al 1990). The second sediment was resuspended in 10 ml alkaline peptone water, pH 8Á6, with subsequent incubation at 25 C for 24 h for Vibrio enrichment (Furniss et al 1979).…”

“…One sediment was resuspended in 10 ml peptone water, pH 7·2, and incubated at 37 °C for 24 h for the enrichment of Aeromonas and Plesiomonas ( Neves et al . 1990 ).…”

Incidence of haemolysin-positive and drug-resistantAeromonas hydrophilain freshly caught finfish and prawn collected from major commercial fishes of coastal South India

“…It has been recognised as a potent cause of food‐borne gastroenteritis [3]. Seafood products are considered to be the prime source for the motile aeromonads [4–6] and fractions of the extracellular proteins (ECP) are associated added concern [7,8].…”

Aeromonas spp. e Plesiomonas shigelloides isoladas a partir de mexilhões (Perna perna) in natura e pré-cozidos no Rio de Janeiro, RJ

“…Após a seleção das cepas citocromo-oxidase positivas, foram realizados testes bioquímicos, baseados na resistência ao agente vibriostático O/129, fermentação de carboidratos e descarboxilação de aminoácidos, a fim de obter a caracterização das espécies de microrganismos isolados [4]. No cômputo geral, os microrganismos mais incidentes foram Aeromonas media (37,10%), A. hydrophila (15,50%), A. caviae (14,80%) e A. veronii biogrupo veronii (11,60%), resultados estes que estão muito próximos daqueles observados por diversos autores [11,13]. Tal fato revela a importância desses microrganismos para a Saúde Pública, tendo em vista seu elevado potencial patogênico para o ser humano.…”

“…no ambiente aquático [11], em amostras de solo de jardim e áreas de recreação [14], a partir de pescado [2,13], e vegetais [12]. Por outro lado, Plesiomonas shigelloides foi isolada em menores proporções, em nosso meio, a partir de pescado [8].…”