Incidence of micronuclei in oral mucosa of users of tobacco products singly or in various combinations

Kayal, J. J.; Trivedi, Amit H.; Davé, Bhavana J.; Nair, Jagadeesan; Nair, Urmila; Bhide, S. V.; Goswami, Umesh; Adhvaryu, Siddharth G.

doi:10.1093/mutage/8.1.31

Cited by 48 publications

(31 citation statements)

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“…This means that at the same age men have a greater number of MN than women. The previous studies indicate that MN in buccal cells show no differences between sexes [Kayal et al, 1993;Gómez-Arroyo et al, 2000]. Thus, the increases observed could be due to a sum of different factors (e.g., drinking, smoking) not uniformly distributed between sexes.…”

Section: Discussionmentioning

confidence: 81%

Occupational exposure to pesticides and cytogenetic damage: Results of a Hungarian population study using the micronucleus assay in lymphocytes and buccal cells

Pastor

Creus

Xamena

et al. 2002

Environ and Mol Mutagen

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The frequency of micronuclei (MN) in peripheral blood lymphocytes and in buccal epithelial cells was used as a biomarker of genotoxic effects resulting from occupational exposure to pesticides. In addition, the cytokinesis-block proliferation index (CBPI) was calculated to detect possible variations in the proliferative kinetics of lymphocytes due to pesticide exposure. This study was performed on 84 pesticide-exposed workers and 65 unexposed controls from Hungary. The pesticide-exposed workers, classified as moderately and highly exposed, were also evaluated separately. Statistical evaluation of the cytogenetic biomarkers indicated that there were no significant differences between pesticide-exposed workers and controls, nor between moderately and highly exposed workers. Nevertheless, the statistical analysis revealed that additional factors such as age, sex, ingestion of raw vegetables, and working as a pesticide applicator affected lymphocyte MN frequency. In addition, age, sex, and smoking affected the frequency of MN in buccal cells. Results from the CBPI analysis showed that the proliferation index decreased with pesticide exposure and that this parameter was also affected by smoking and by the gender of individuals. The results of this study indicate no significant increase in MN in this group of Hungarian workers; however, the reduced CBPI in the highly exposed population suggests a possible genotoxic effect of pesticide exposure.

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Section: Discussionmentioning

confidence: 81%

Occupational exposure to pesticides and cytogenetic damage: Results of a Hungarian population study using the micronucleus assay in lymphocytes and buccal cells

Pastor

Creus

Xamena

et al. 2002

Environ and Mol Mutagen

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“…The data presented by Wu et al [10] revealed that cigarette smoking did not increase the number of micronuclei in smokers when compared with controls. Kayal et al [11] investigated micronuclei in buccal mucosa cells of persons who chewed indigenous products (areca nut, mava, tamol, tobacco with lime, dry snuff, or mashery), but not tobacco cigarettes. Motgi et al [12] have demonstrated that total numbers of micronucleated cells were significantly lower in non-tobacco users when compared with tobacco users, but such data were obtained by using non-specific DNA stains (Papanicolaou stain).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

et al. 2015

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Objective: Nowadays, much attention has been focused on the search for new non-invasive methodologies able to predict malignant transformation of oral mucosa cells. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers in buccal mucosa cells. Study Design: A total of 24 young, healthy smokers and 14 non-smokers were included in this setting. Individuals had epithelial cells from the cheek mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. Results: Smokers presented more (p < 0.05) micronucleated oral mucosa cells than non-smokers. Tobacco smoke was not able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis. Conclusion: In summary, these data indicate that the cigarette is able to induce micronuclei in oral mucosa cells, so the micronucleus test is a suitable method for predicting oral cancer risk.

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“…In the case of epithelial tissue, effective techniques have not yet been developed for preparing metaphase chromosomes for conventional aberration analysis. The MN assay, however, is an effective method for detecting chromosomal aberrations in exfoliated epithelial cells [Tolbert et al, 1992;Kayal et al, 1993;Pastor et al, 2001;Huvinen et al, 2002;Guzman et al, 2003].…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of occupational exposure to wood dust: Micronucleus frequency and nuclear changes in exfoliated buccal mucosa cells

Çelık

Kanık

2006

Environ and Mol Mutagen

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Occupational exposure to wood dust is associated with the occurrence of nasal cancer. In this study, we investigated micronuclei and nuclear changes (NCs: binucleates, karyorrhexis, karyolysis, and the "broken egg" effect) in exfoliated buccal cells of 20 workers exposed to wood dust and 20 age- and sex-matched controls. Micronucleus frequency and the frequency of each of the NCs were significantly higher for wood workers than controls (P < 0.01). Cigarette smoking was associated with increased frequencies of micronuclei and NCs in the buccal mucosa epithelium cells of both the control and exposed groups. Our findings indicate that buccal cells of wood workers display increased levels of genotoxicity and toxicity, and that these biomarker responses may be related to the increased cancer risk among wood workers.

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Incidence of micronuclei in oral mucosa of users of tobacco products singly or in various combinations

Cited by 48 publications

References 0 publications

Occupational exposure to pesticides and cytogenetic damage: Results of a Hungarian population study using the micronucleus assay in lymphocytes and buccal cells

Occupational exposure to pesticides and cytogenetic damage: Results of a Hungarian population study using the micronucleus assay in lymphocytes and buccal cells

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

Genotoxicity of occupational exposure to wood dust: Micronucleus frequency and nuclear changes in exfoliated buccal mucosa cells

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