Incidence of mastitis and activity of milk neutrophils in Tharparkar cows reared under semi-arid conditions

Alhussien, Mohanned Naif; Manjari, P.; Mohammed, Seid; Sheikh, Aasif Ahmad; Reddi, Srinu; Dixit, Satpal; Dang, Ajay Kumar

doi:10.1007/s11250-016-1068-8

Cited by 40 publications

(23 citation statements)

References 19 publications

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“…In addition, a positive correlation between SCC and Na content of milk was detected in the current study. These results agree with those previously observed for cows [35,36]. For example, Summer et al [35] found higher contents of Na in milk with more than 5.6021 log 10/mL than in milk with less than 5.6021 log 10/mL.…”

Section: Discussionsupporting

confidence: 92%

Parenteral Cu Supplementation of Late-Gestating and Lactating Iberian Red Deer Hinds Fed a Balanced Diet Reduces Somatic Cell Count and Modifies Mineral Profile of Milk

Serrano

García

Landete‐Castillejos

et al. 2020

Animals

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This study describes the effects that Cu supplementation of late-gestating and lactating females (hinds) of Iberian red deer fed a balanced diet have on milk production, composition, and somatic cell count (SCC). Experimental hinds (n = 9) were subcutaneously injected every 42 days with Cu (0.83 mg Cu/kg body weight) from day 202 of gestation until the end of lactation (week 18). Control hinds (n = 8) were injected with a physiological saline solution with the same volume and at the same frequency as the experimental group. Copper supplementation decreased the SCC from 1.64 to 1.36 log 10/mL (p = 0.003) and modified the milk mineral profile. In particular, milk from hinds supplemented with Cu had more Ca (p = 0.02), Mg (p = 0.06), and K (p = 0.03) than milk from control hinds. However, Cu supplementation did not influence the Cu content of milk. Therefore, it can be concluded that Cu supplementation of hinds fed a balanced diet from late-gestation to the end of lactation could be a good strategy to reduce SCC and modify the mineral profile of milk increasing its Ca content.

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Section: Discussionsupporting

confidence: 92%

Parenteral Cu Supplementation of Late-Gestating and Lactating Iberian Red Deer Hinds Fed a Balanced Diet Reduces Somatic Cell Count and Modifies Mineral Profile of Milk

Serrano

García

Landete‐Castillejos

et al. 2020

Animals

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“…In our study, we showed that MEC and the percentage of CD11b positive leukocytes increase in bacteriological positive milk samples than negative. This result agrees also with Alhussien et al [ 24 ] that observed a significant increase of MEC in cows with clinical mastitis and significant increase in mRNA expression of CD11b on isolated milk PMN from cows with sub-clinical and clinical mastitis.…”

Section: Discussionsupporting

confidence: 93%

Flow Cytometry-Detected Immunological Markers and on Farm Recorded Parameters in Composite Cow Milk as Related to Udder Health Status

Matteis

Grandoni

Scatà

et al. 2020

Veterinary Sciences

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Flow cytometry is a powerful technology used in many fields of cell biology. It is also used as a routine method to count somatic cells in milk and to characterize bovine milk leukocytes. In this study, we used flow cytometry to simultaneously assess the viability, the percentage of the single subsets of leukocytes and to quantify the expression of CD11b, an immunological marker of cell activation status. Immunological markers were then related with on farm recorded parameters as milk electrical conductivity (MEC) and average milk flow rate (MFR). Composite milk samples were collected from 43 cows, nine of which had naturally infected udders and 34 of which had no infected udders. First, the milk samples were classified according to bacteriological test in positive and negative. The results showed that the negative samples to bacteriological test had: (i) significantly higher percentages of live lymphocytes; (ii) significantly lower percentages of CD11b+ leukocytes; (iii) significantly lower MEC and higher MFR values. Then, samples were classified in three groups according to somatic cell count (SCC): Group A (n = 15), samples with SCC ≤ 100,000 cells/mL, all negative to bacteriological analysis; Group B (n = 11), samples with 100,000 < SCC < 300,000 cells/m, with four samples positive to bacteriological analysis; Group C (n = 17), samples with SCC ≥ 300,000 cell/mL with five samples positive to bacteriological analysis. Multivariate discriminant analysis was used to verify which flow cytometry immunological markers and on farm recorded parameters could better discriminate among the different groups of SCCs. Linear discriminant analysis (LDA) indicated that 5 of the 10 parameters could best be used to reveal the differences between positive and negative samples among the considered groups of SCCs. Furthermore, the Canonical discriminant analysis (CDA) indicated that composite milk samples with different SCC and infection status were clearly separated from each other in a two-dimensional space. In conclusion, the study highlighted that: (1) the conventional flow cytometry analysis applied on milk samples is a useful tool to investigate immunological parameters as potential indicators of udder health; (2) the combined evaluation of live milk leukocytes and recorded farm parameters could be useful to assess udder health status in dairy cows. The results obtained from this pilot study on few data require new and larger trials to be confirmed.

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“…There is an increase in the percentage of lymphocytes, followed by macrophages and neutrophils in normal mammary quarters [ 20 ]. However, reports of macrophage having the maximum percentage in normal milk SCC are also there [ 21 , 22 ]. Resident neutrophil in milk with low SCC might modulate the initial steps of dynamic immune defense of the udder, and percentage change in neutrophils in conjunction with SCC might provide a more reliable method for assessing the quality of milk in buffaloes [ 23 ].…”

Section: Milk Differential Leukocyte Counts (Dlcs)mentioning

confidence: 99%

“…SCC variation has been noted between various breeds of cows [ 14 , 22 , 27 , 56 - 58 ] as presented in Figure-3 . The high-producing cattle breeds such as Brown Swiss and Holstein have a higher presence of SCC/ml in milk.…”

Section: Factors Affecting Milk Sccmentioning

confidence: 99%

Milk somatic cells, factors influencing their release, future prospects, and practical utility in dairy animals: An overview

2018

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Milk somatic cells (SCs) are a mixture of milk-producing cells and immune cells. These cells are secreted in milk during the normal course of milking and are used as an index for estimating mammary health and milk quality of dairy animals worldwide. Milk SC is influenced by cow productivity, health, parity, lactation stage, and breed of an animal. Any change in environmental conditions, poor management practices, and also stressful conditions significantly increases the amount of SC coming in milk. Better hygiene and proper nutrition help in reducing milk SC. Milk with low SC means better milk products with a longer shelf life. The present review describes the role of SCs (both secretory and immune) in milk, their role in maintaining the integrity of the mammary gland, and factors affecting their release in milk. This information may help to reduce milk somatic cell counts (SCCs) and to establish differential SCC standards.

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Incidence of mastitis and activity of milk neutrophils in Tharparkar cows reared under semi-arid conditions

Cited by 40 publications

References 19 publications

Parenteral Cu Supplementation of Late-Gestating and Lactating Iberian Red Deer Hinds Fed a Balanced Diet Reduces Somatic Cell Count and Modifies Mineral Profile of Milk

Parenteral Cu Supplementation of Late-Gestating and Lactating Iberian Red Deer Hinds Fed a Balanced Diet Reduces Somatic Cell Count and Modifies Mineral Profile of Milk

Flow Cytometry-Detected Immunological Markers and on Farm Recorded Parameters in Composite Cow Milk as Related to Udder Health Status

Milk somatic cells, factors influencing their release, future prospects, and practical utility in dairy animals: An overview

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