Incidence of <i>Prevotella intermedia</i> and <i>Prevotella nigrescens</i> in Periodontal Health and Disease

Maeda, Nobuyo; Okamoto, Masaaki; Kondo, Kenichiro; Ishikawa, Hiroyuki; Osada, Reiko; Tsurumoto, Akihisa; Fujita, Hiroshi

doi:10.1111/j.1348-0421.1998.tb02328.x

Cited by 49 publications

(34 citation statements)

References 29 publications

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“…Several studies have reported the increased incidence of P. intermedia in periodontal lesions, while P. nigrescens seems to be associated with endodontic infection, adult gingivitis and healthy gingival sites [6^10]. We have also observed that P. intermedia is predominant in periodontal disease, whereas P. nigrescens was predominant in periodontally healthy subjects [11]. However, some studies have reported contradictory results regarding the occurrence of Prevotella in the oral cavity [12,13].…”

Section: Introductionsupporting

confidence: 54%

Hemolytic and hemagglutinating activities of Prevotella intermedia and Prevotella nigrescens

Okamoto

1999

FEMS Microbiology Letters

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A total of 91 isolates of Prevotella intermedia or Prevotella nigrescens from subgingival sites were identified by PCR using primers specific for sequences of 16S rRNA. The hemolytic and hemagglutinating activities of the P. intermedia isolates exhibited significantly higher levels compared to those of the P. nigrescens isolates by quantitative analysis. The hemagglutinin gene (phg) was found in 23 of 26 P. intermedia isolates (88.5%), whereas it was found in only two of 44 isolates (4.5%) of P. nigrescens. The high hemolytic and hemagglutinating activities of P. intermedia may be involved in the pathogenicity of P. intermedia in the progression of periodontal disease. ß

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Section: Introductionsupporting

confidence: 54%

Hemolytic and hemagglutinating activities of Prevotella intermedia and Prevotella nigrescens

Okamoto

1999

FEMS Microbiology Letters

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“…Faecalibacterium are commensal human gut bacteria with apparent anti-inflammatory activity (Sokol et al, 2008(Sokol et al, , 2009, which may ultimately be investigated with a view to its use as a probiotic to treat Crohn's disease, colitis, and irritable bowel syndrome (Kirjavainen et al 2001). Prevotella are commensal microbes in rumen and hind gut of cows and sheep and in humans, where they help the breakdown of proteins and carbohydrates (Yildirim et al, 2010); it is also associated with periodontal disease in humans (Maeda et al, 1998). Although Catenibacterium are also associated with the human gut, little is known about this genus (Kageyama and Benno, 2000).…”

Section: Discussionmentioning

confidence: 99%

The microbial content of raw and pasteurized cow milk as determined by molecular approaches

Quigley

McCarthy

O'Sullivan

et al. 2013

Journal of Dairy Science

137

106

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The microbial composition of raw and pasteurized milk is assessed on a daily basis. However, many such tests are culture-dependent, and, thus, bacteria that are present at subdominant levels, or that cannot be easily grown in the laboratory, may be overlooked. To address this potential bias, we have used several culture-independent techniques, including flow cytometry, real-time quantitative PCR, and high-throughput DNA sequencing, to assess the microbial population of milk from a selection of commercial milk producers, pre- and postpasteurization. The combination of techniques employed reveals the presence of a previously unrecognized and diverse bacterial population in unpasteurized cow milk. Most notably, the use of high-throughput DNA sequencing resulted in several bacterial genera being identified in milk samples for the first time. These included Bacteroides, Faecalibacterium, Prevotella, and Catenibacterium. Our culture-independent analyses also indicate that the bacterial population of pasteurized milk is more diverse than previously appreciated, and that nonthermoduric bacteria within these populations are likely to be in a damaged, nonculturable form. It is thus apparent that the application of state-of-the-art approaches can provide a detailed insight into the bacterial composition of milk and could potentially be employed in the future to investigate the factors that influence the composition of these populations.

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“…This is a precursor to progression to periodontitis. This is buttressed by the fact that P. intermedia has been known to increase the activity of degradative enzymes under certain conditions, and supports the progression to periodontitis [12].…”

Section: Discussionmentioning

confidence: 99%

The Bacteriology of the Supragingival Plaque of Child Dental Patients in Kuwait

Salako¹,

Rotimi

Preeta³

et al. 2004

Med Princ Pract

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Objective: To investigate the bacterial flora of the gingival plaques of pediatric dental patients in order to establish baseline data for future studies on the complex microbiota of the oral cavity of this group of child dental patients. Subjects and Methods: Forty subjects, aged 3–12 years, were enrolled and investigated after informed consent was obtained. During the patients’ first visit to the dental clinic supragingival plaque samples were collected from the gingival area of buccal and lingual tooth surfaces of all primary and permanent molar teeth using sterile curettes. Serially diluted samples were inoculated onto a set of selective and nonselective media and then incubated at the appropriate atmospheric conditions and temperatures. Representative colonies of isolates were identified by standard methods, including hemolytic characteristics on blood agar and profiling on API 20S and API 20A as appropriate. Results: A total of 183 isolates (123 aerobes and 60 anaerobes) were recovered from the 40 patients with a mean count of 3 per sample of aerobes compared to 1.5 per sample for the anaerobes. Streptococcus sanguis was the predominant aerobic species (23.6%) isolated from 29 (72.5%) of the 40 patients, followed by Streptococcus mitis, 19.5 and 60%, Streptococcus salivarius, 17.1 and 52.5% and Streptococcus mutans, 17.1 and 52.5%. The frequency of isolation of anaerobic bacteria was Prevotella spp. 50% from 30 (75%) of the 40 patients, Fusobacterium spp., 18.3 and 27.5%, Bacteroides spp., Porphyromonas spp. and Peptostreptococcus spp., 6.7 and 10%, 6.7 and 10%, and 5 and 7.5%, respectively. Prevotella intermedia was the single most common species. Conclusion: The results show that there were more aerobic/facultative anaerobic bacteria than the obligate anaerobes in the dental plaque of the pediatric dental patients in Kuwait. The high prevalence of the pioneering streptococci as well as black-pigmented Prevotella spp. and Fusobacterium spp. indicates that the dental plaques of the children were in the developing stage, a precursor to the development of periodontal diseases.

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Incidence of Prevotella intermedia and Prevotella nigrescens in Periodontal Health and Disease

Cited by 49 publications

References 29 publications

Hemolytic and hemagglutinating activities of Prevotella intermedia and Prevotella nigrescens

Hemolytic and hemagglutinating activities of Prevotella intermedia and Prevotella nigrescens

The microbial content of raw and pasteurized cow milk as determined by molecular approaches

The Bacteriology of the Supragingival Plaque of Child Dental Patients in Kuwait

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