Incidence of Human Cytomegalovirus Viremia among Egyptian Hepatitis C - Patients with Hepatocellular Carcinoma

Abstract

Background: Hepatocellular carcinoma (HCC) is the major hepatic complication that may arise after many years of hepatitis C virus (HCV) infection. In Egypt, HCV represents the major health problem, also human cytomegalovirus (HCMV) is known as one of the highest un-resolved latent infections among general population. HCMV viremia in the co-infection with HCV may cause life threatening in HCC patients. Our study aimed to detect HCMV viremia in HCV-patients experienced HCC, and to investigate its role in disease worsening. Methods: In HCC patients, HCV-RNA viral load was determined by real-time polymerase chain reaction. In HCV-HCC patients, HCMV-DNA was detected by amplification of gB gene region using nestedpolymerase chain reaction. Results: HCMV-DNA was detected in 4/73 in control subjects with prevalence rate of 5.4%, whereas HCMV-DNA was recorded in 24/75 HCV-HCC patients with prevalence rate of 32 %. Data on the level of alpha feto protein (AFP) was available for 59 out of 75 HCV-HCC patients, this enabled us to differentiate between low risk HCC group of 40/59 patients with AFP < 500 ng/ml, from them HCMV-DNA was detected in 14/40 patients with prevalence rate 35%, and high risk HCC group of 19/59 patients with AFP > 500 ng /ml, from them HCMV-DNA was reported in 9/19 patients with prevalence rate 47.37%. High significant prevalence rate of HCMV-DNA (P < 0.001) among control and HCC subjects was reported. Significant change in HCMV-DNA prevalence between low and high risk HCC groups could not be achieved but tendency of higher prevalence rate in HCMV-DNA was observed towards HCC high risk group of patients. Conclusion: we illustrated information about HCMV/ HCV co-infection in HCC patients, which referred to the association of HCMV viremia with HCV-HCC patients, as well as the tendency of elevation in HCMV viremia from low to high risk HCC patients depending on AFP threshold of 500 ng/ml.

Serological and Immunohistochemical Prevalence of Human Cytomegalovirus Co-infection among Hepatitis C Virus Patients Admitted to Kafer El Shiekh Liver and Heart Institute, Egypt

“…Moreover, liver cirrhosis resulted from chronic HCV infection is the main cause for liver transplantation [24]. CMV is not only the common cause of morbidity after liver transplant, but also it may interact and accelerate HCV pathogenesis [25][26][27][28][29][30][31]. It was documented that the possibility of achieving sustained virologic response to ribavirin plus pegylated interferon treatment in chronic HCV patients could dramatically diminish during CMV infection [32].…”