Incidence of Hospital-Acquired Bacterial Pneumonia and Its Resistance Profiles in Patients Admitted to Intensive Care Unit

Boostani, Vahid; Dehghan, Farzaneh; Karmostaji, Afsaneh; Zolghadri, Nader; Shafii, Afsaneh

doi:10.5539/gjhs.v9n3p73

Cited by 3 publications

(1 citation statement)

References 26 publications

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“…The rate of HAP occurs 21 cases per 1,000 hospital admissions [33]. In various studies, gram-negative bacteria are more prevalent in HAP than gram-positive bacteria [34][35][36]. In the present study, Gram-negative bacteria (A.baumannii, P.aeruginosa, E. coli and K. pneumoniae) were isolated in 72.2%, which was in accordance with previous studies such as Bozorgmehr [35][36][37].The frequency HAP causing bacteria may vary by hospital, geographic area, patient status, patient's immune system, and health status.…”

Section: Discussionmentioning

confidence: 99%

Identification and Detection of Pathogenic Bacteria From Patients With Hospital Acquired Pneumonia in Southwest of Iran; Evaluation of Biofilm Production and Molecular Typing of Bacterial Isolates

Mazloomirad

Hasanzadeh

Sharifi

et al. 2021

Preprint

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BackgroundHospital acquired pneumonia (HAP) HAP is the most second nosocomial infection and the most common infection in the intensive care unit. The aim of the present study was to determine the prevalence of pathogenic bacteria, their biofilm formation and molecular typing in sputum samples from patients with HAP in southwest of Iran.MethodsIn this cross-sectional study, 58 patients with HAP were included in this study. Clinical samples including sputum andendotracheal aspirate collected from each patient under aseptic conditions and were sent to the Microbiology Laboratory.Isolation and detection of bacteria were performed by culture and polymerase chain reaction methods.Biofilm formation was evaluated using by Congo red Agar or microtiter plate assay.A rep- PCR typing method was used for typing of gram-negative bacteria. MRSA isolates were typed using SCCmec typing method. Data were analyzed by SPSS software version 15 and chi-square test.ResultsUsing culture method, bacteria identified in 52 (89.7 %) of patients. Acinetobacter baumannii was the most prevalent (37%) organism and followed by Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. All of the A. baumannii and P. aeruginosa were biofilm producer. 12 cases (80%) of S. aureus isolates were MRSA and 91.6% of MRSA were SCCmec type III. The rep -PCR analysis showed 7 different patterns in 20 A. baumannii, 6 patterns in 13 P. aeruginosa and 4 patterns in 6 E. coli. Most of the A. baumanii isolates were multi drug resistant. ConclusionA. baumanii was more prevalent in VAP, while S. aureus as a major pathogen in N- VAP that maybe related to tendency of A. baumannii to the aquatic environment. Biofilm production ability in bacteria increased antibiotic resistance, development of chronic and persistent infection in HAP. Based on rep- PCR typing method we concluded that bacteria transmitted from patients or health care workers among different wards of hospital. Colistin can be used as a treatment in gram negative MDR isolates.

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Section: Discussionmentioning

confidence: 99%

Identification and Detection of Pathogenic Bacteria From Patients With Hospital Acquired Pneumonia in Southwest of Iran; Evaluation of Biofilm Production and Molecular Typing of Bacterial Isolates

Mazloomirad

Hasanzadeh

Sharifi

et al. 2021

Preprint

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Diagnosis of nonventilated hospital-acquired pneumonia: how much do we know?

Ranzani

Pascale

Park

2018

Current Opinion in Critical Care

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Identification and detection of pathogenic bacteria from patients with hospital-acquired pneumonia in southwestern Iran; evaluation of biofilm production and molecular typing of bacterial isolates

et al. 2021

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Background Hospital-acquired pneumonia (HAP) is the second most common nosocomial infection in intensive care units (ICUs). The present study aims to determine the prevalence of pathogenic bacteria, their biofilm formation, and molecular typing from patients with HAP in southwestern Iran. Methods Fifty-eight patients with HAP participated in this cross-sectional study. Sputum and endotracheal aspirate were collected from each patient for isolation and detection of bacteria. Biofilm formation was evaluated using Congo red agar or Microtiter plate assay. The antimicrobial susceptibility patterns of the isolates were investigated. The multiplex polymerase chain reaction (M-PCR) technique was used to determine the Staphylococcal Cassette Chromosome mec (SCCmec) types of methicillin-resistant Staphylococcus aureus (MRSA) strains. All S. aureus isolates were typed using the agr typing method. A repetitive element sequence-based PCR (rep-PCR) typing method was used for typing of Gram-negative bacteria. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software version 15 and the chi-square test. Results Bacteria were isolated in 52 (89.7%) of patients. Acinetobacter baumannii (A. baumannii) was the most prevalent organism (37%), followed by S. aureus, Pseudomonas aeruginosa (P. aeruginosa), and Escherichia coli (E. coli). Using the PCR method, 56 bacteria were detected. A. baumannii was the most prevalent (35.7%) organism. A. baumannii and P. aeruginosa were biofilm-producing. All Gram-negative isolates were colistin-sensitive, and most of the A. baumannii isolates were multidrug-resistant (MDR). MRSA was identified in 12 (80%) S. aureus isolates, and 91.6% of MRSA were SCCmec type III. The agr type III was the most predominant. The rep-PCR analysis showed seven different patterns in 20 A. baumannii, six patterns in 13 P. aeruginosa, and four patterns in 6 E. coli. Conclusion A. baumannii was more prevalent than S. aureus in ventilator-associated pneumonia (VAP), while S. aureus is a major pathogen in non-ventilator hospital-acquired pneumonia (NV-HAP), possibly due to the tendency of the former to aquatic environments. Based on the rep-PCR typing method, it was concluded that bacteria were transmitted from patients or healthcare workers among different wards. Colistin can be used as a treatment in Gram-negative MDR isolates.

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Incidence of Hospital-Acquired Bacterial Pneumonia and Its Resistance Profiles in Patients Admitted to Intensive Care Unit

Cited by 3 publications

References 26 publications

Identification and Detection of Pathogenic Bacteria From Patients With Hospital Acquired Pneumonia in Southwest of Iran; Evaluation of Biofilm Production and Molecular Typing of Bacterial Isolates

Identification and Detection of Pathogenic Bacteria From Patients With Hospital Acquired Pneumonia in Southwest of Iran; Evaluation of Biofilm Production and Molecular Typing of Bacterial Isolates

Diagnosis of nonventilated hospital-acquired pneumonia: how much do we know?

Identification and detection of pathogenic bacteria from patients with hospital-acquired pneumonia in southwestern Iran; evaluation of biofilm production and molecular typing of bacterial isolates

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