Incidence of Fecal Coliforms and Serovars of Enteropathogenic Escherichia Coli in Naturally Contaminated Cheese

Abbar, F. M.

doi:10.4315/0362-028x-51.5.384

Cited by 6 publications

(4 citation statements)

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“…A variety of animals, such as cattle, goats, sheep, chickens, and pigeons are known to be vehicles of EPEC strains [26]; these, as well as human sources, could likely be the origin of the serogroups we found in our samples. Interestingly, our results were comparable to reported prevalence of EPEC in dairy products where O111, O86, O125, O119, and O111, O126, O128, O26, O25, O125 were commonly isolated in Iraq [27] and Egypt [28], respectively. Such observations indicate the likelihood of the agricultural production system as a primary source of contamination of raw vegetables.…”

Section: Serotypingsupporting

confidence: 89%

Prevalence of antimicrobial-resistant Escherichia coli from raw vegetables in Lebanon

Faour-Klingbeil

Kuri

Fadlallah

et al. 2016

J Infect Dev Ctries

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Introduction: Fresh produce has been implicated in a number of documented outbreaks of foodborne illness caused by bacteria, viruses, and parasites. Shiga toxin-producing Escherichia coli (STEC) have been detected on vegetables, raising concerns about the prevalence of E. coli contamination in produce, which can take place at various points from farm to fork. This study aimed to detect the presence of STEC and multidrug-resistant (MDR) E. coli on fresh vegetables and water from different sources along the fresh produce supply chain in Lebanon. Methodology: E. coli isolates (n = 60) were group serotyped using trivalent antisera (trivalent 1 [O111+O55+O26], trivalent 2 [O86+O119+O127], trivalent 3 [O125; O126; O128], and trivalent 4 [O114+O124+O142]) and tested for stx1 and stx2 genes by polymerase chain reaction (PCR) assay. Resistance to antimicrobial agents was determined using the disk diffusion method. Results: The virulence genes stx1 and stx2 were not detected in any of the isolates. However, 60% of the isolates were MDR and predominantly observed in trivalent 2 (32%). It is postulated that the inadequate post-harvest washing contributed to transmission of antimicrobial-resistant E. coli at wholesale and retail levels. Fresh vegetables harbor MDR E. coli and their consumption poses risks of increasing the reservoir of antimicrobial resistance in the intestines of the Lebanese population. Conclusions: Greater emphasis should be placed on vigilant sanitation measures at the consumption level, and effective national risk mitigation strategies are crucial to minimize fecal contamination in the early stages of production, particularly in the post-harvest washing processes.

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Section: Serotypingsupporting

confidence: 89%

Prevalence of antimicrobial-resistant Escherichia coli from raw vegetables in Lebanon

Faour-Klingbeil

Kuri

Fadlallah

et al. 2016

J Infect Dev Ctries

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“…Most prevalent serogroups found in milk and milk products in Egypt are O111, O126, O128, O26, O25 and O125 [20,28]. In Iraq, four EPEC serogroups, including O111, O86, O125 and O119, are commonly isolated from cheese samples [29]. In the present investigation all EPEC serogroups were found in the soft variety sweetmeat samples tested and O55 was the most frequent one (23.66%).…”

Section: Discussionmentioning

confidence: 78%

“…In the present investigation all EPEC serogroups were found in the soft variety sweetmeat samples tested and O55 was the most frequent one (23.66%). Contamination of dairy products by EPEC strains has been investigated and O126, O128, O25 and O125 were isolated [20,29]. It was observes that O55 EPEC serogroup is one of most prevalent in children diarrhea in Brazil [30].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Enteropathogenic Escherichia coli Isolated from Chhana Based Indian Sweets in Relation to Public Health

2010

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Chhana based milk products viz. rossogolla, kanchagolla,narampak sandesh and karapak sandesh are very popular in eastern part of India and gaining popularity in other parts of the country. A wide variation in manufacture method, microbial quality and shelf-life of these traditional milk products were observed by previous research. The aim of the present study was to determine the prevalence of contamination of chhana based milk products available in Kolkata city with Enteropathogenic Escherichia coli (EPEC) serogroups. Random samples of different chhana based milk products were collected from different parts of Kolkata city in aseptic condition, cultured in selective media and examined for biochemical tests. Among 240 samples, E. coli was isolated from 67 (27.91%) of them. Potential EPEC was present in 52 samples (21.66%) and 55 of the isolates were EPEC. Eleven serogroups were identified viz. O26, O55, O111, O119, O114, O125, O142, O86, O126, O127, O128. Among all these serogroups, O55 (23.66%) was the most prevalent. Though recent studies on virulence factors indicate that not all strains serologically classified as EPEC are able to attaching/effacing lesion, it is believed that the isolation of EPEC serogroups from chhana based milk products represent a potential risk for public health particularly children, as well as an indicative of the presence of other enteropathogens. Considering the public health importance of sweetmeat consumers, the product should be prepared hygienically reducing the microbial load present in it. The result indicates that strict preventive measures should be adopted to ensure contamination free sweetmeats for the safety of the consumers.

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“…Other studies on milk and/or dairy products showed similar results both in Brazil 3,6,9,17 and other countries. 1,5,8 It was observed that types B and C milk of brand III had a high coliform level (Figure) although EPEC strains prevailed in samples of brand II (Table 3). EPEC strains were also found in samples of brand I ( Table Table 2 Figure -Enumeration of total and fecal coliforms above the levels permitted by SNVS 12 (1997) in pasteurized milk samples.…”

Section: Discussionmentioning

confidence: 99%

Isolation and serological identification of enteropathogenic Escherichia coli in pasteurized milk in Brazil

et al. 2001

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ObjectiveTo evaluate the microbiological quality of pasteurized milk commercialized in Rio de Janeiro, Brazil, and determine serologically enteropathogenic Escherichia coli (EPEC) strains in E. coli isolates obtained from milk samples. Methods Ninety samples of pasteurized milk -types B and C -of three different commercial brands, purchased in supermarkets and bakeries in Rio de Janeiro, were examined. The amount of total and fecal coliform bacteria was estimated using the Most Probable Number technique. Mesophilic, psychrotrophic, and thermoduric microorganism counts were determined by the Standard Plate Count technique. Isolation and identification of E. coli were carried out using conventional physiological tests. Commercial antisera were used for serological characterization of EPEC. ResultsThe three milk brands analyzed revealed bacterial counts above the regulated values of the Brazilian government. It was found that among 208 strains of E. coli isolated, 46 (22.1%) were serologically classified as EPEC. The most common EPEC serogroup was O55 (15.2%). Conclusions Though recent studies on virulence factors indicate that not all strains serologically classified as EPEC are able to attaching/effacing lesion, it is believed that the isolation of EPEC serogroups from pasteurized milk represent a potential risk for children, as well as an indicative of the presence of other enteropathogens. Resumo Objetivos Avaliar a qualidade microbiológica de leite pasteurizado comercializado, isolar e identificar sorologicamente cepas de Escherichia coli enteropatogênica clássica (EPEC) nesse alimento. Métodos Foram estudadas 90 amostras de leite pasteurizado, de três marcas comerciais diferentes, dos tipos B e C, obtidas em padarias e supermercados do Rio de Janeiro,3 7 6 3 7 6 3 7 6 3 7 6 3 7

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Incidence of Fecal Coliforms and Serovars of Enteropathogenic Escherichia Coli in Naturally Contaminated Cheese

Cited by 6 publications

References 0 publications

Prevalence of antimicrobial-resistant Escherichia coli from raw vegetables in Lebanon

Prevalence of antimicrobial-resistant Escherichia coli from raw vegetables in Lebanon

Prevalence of Enteropathogenic Escherichia coli Isolated from Chhana Based Indian Sweets in Relation to Public Health

Isolation and serological identification of enteropathogenic Escherichia coli in pasteurized milk in Brazil

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