Incidence of donor site morbidity following harvesting from iliac crest or RIA graft

Calori, G.M.; Colombo, M.; Mazza, Emílio; Mazzola, S.; Malagoli, E.; Mineo, Giuseppe

doi:10.1016/j.injury.2014.10.034

Cited by 125 publications

(76 citation statements)

References 50 publications

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“…[1] Despite its safety and effectiveness, bone graft harvesting results in donor site pain and inflammation. There is a limited quantity of donor tissue available, [2] necessitating strategies that eliminate the need for autologous bone grafts. Cell-based approaches utilizing mesenchymal stem cells (MSCs) are under investigation for bone repair of atrophic nonunions, craniomaxillofacial defect repair, and hard palate reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Bone Morphogenetic Protein‐2 Promotes Human Mesenchymal Stem Cell Survival and Resultant Bone Formation When Entrapped in Photocrosslinked Alginate Hydrogels

Vollmer

Refaat

et al. 2016

Adv Healthcare Materials

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There is a substantial need for methods that prolong cell persistence and enhance functionality in situ to enhance cell-based tissue repair. Bone morphogenetic protein-2 (BMP-2) is often used at high concentrations for osteogenic differentiation of mesenchymal stem cells (MSCs) but can induce apoptosis. Biomaterials facilitate the delivery of lower doses of inductive molecules, potentially reducing side effects and localizing materials at the target site. Photocrosslinked alginate hydrogels (PAHs) can deliver osteogenic materials to irregular-sized bone defects, providing improved control over material degradation compared to ionically-crosslinked hydrogels. We hypothesized that the delivery of human MSCs and BMP-2 from a PAH would increase cell persistence by reducing apoptosis, while promoting osteogenic differentiation and enhancing bone formation compared to MSCs in PAHs without BMP-2. BMP-2 significantly decreased apoptosis and enhanced survival of photoencapsulated MSCs, while simultaneously promoting osteogenic differentiation in vitro. Bioluminescence imaging revealed increased MSC survival when implanted in BMP-2 PAHs over 4 weeks. Bone defects treated with MSCs in BMP-2 PAHs demonstrated 100% union as early as 8 weeks and significantly higher bone volumes at 12 weeks, while defects with MSC-entrapped PAHs alone did not fully bridge. This study demonstrates that transplantation of MSCs with BMP-2 in PAHs achieves robust bone healing, providing a promising platform for use in bone repair.

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Section: Introductionmentioning

confidence: 99%

Bone Morphogenetic Protein‐2 Promotes Human Mesenchymal Stem Cell Survival and Resultant Bone Formation When Entrapped in Photocrosslinked Alginate Hydrogels

Vollmer

Refaat

et al. 2016

Adv Healthcare Materials

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“…Nevertheless, the therapeutic effect for large cartilage damage is not satisfactory, as autografts suffer from the inadequate tissue availability and the associated morbidity of the donor site, and allografts are limited by transplant rejection23. The tissue engineering for the treatment of articular cartilage defects presents a promising strategy4, however, many problems remain.…”

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confidence: 99%

Comparative evaluation of leukocyte- and platelet-rich plasma and pure platelet-rich plasma for cartilage regeneration

Yin

Zhang

et al. 2017

Sci Rep

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Platelet-rich plasma (PRP) has gained growing popularity in the treatment of articular cartilage lesions in the last decade. However, the potential harmful effects of leukocytes in PRP on cartilage regeneration have seldom been studied in vitro, and not at all in vivo yet. The objective of the present study is to compare the effects of leukocyte- and platelet-rich plasma (L-PRP) and pure platelet-rich plasma (P-PRP) on cartilage repair and NF-κB pathway, in order to explore the mechanism underlying the function of leukocytes in PRP in cartilage regeneration. The constituent analysis showed that P-PRP had significantly lower concentrations of leukocytes and pro-inflammatory cytokines compared with L-PRP. In addition, cell proliferation and differentiation assays indicated P-PRP promoted growth and chondrogenesis of rabbit bone marrow mesenchymal stem cells (rBMSC) significantly compared with L-PRP. Despite similarity in macroscopic appearance, the implantation of P-PRP combining rBMSC in vivo yielded better cartilage repair results than the L-PRP group based on histological examination. Importantly, the therapeutic effects of PRP on cartilage regeneration could be enhanced by removing leukocytes to avoid the activation of the NF-κB pathway. Thus, PRP without concentrated leukocytes may be more suitable for the treatment of articular cartilage lesions.

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“…The main complications of removal of cancellous bone from the iliac crest are infections, development of hematomas, fractures, hypertrophic scars, and chronic pain from the extraction area (51).…”

Section: Complicationsmentioning

confidence: 99%

Non-Union Current Treatment Concept

2016

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Context: This article wants to give a current concept for the challenging decision for conservative or operative treatment strategies of nonunions according to the principles of 'diamond concept' and aspects that have to be attended. Evidence Acquisition: Between February 2010 and March 2014, 424 patients with non-unions were treated at Heidelberg university hospital. This database has been analyzed at least one year after the treatment. The analysis and the experience in surgery and treatment of non-unions as well as present literature were prepared for this review as a current concept. Results: If an atrophic non-union is suggested, reosteosynthesis and biological stimulation is required. A revision surgery of autologous transplantation of cancellous bone from the iliac crest is often enough. Alternatively, reamer-irrigator-aspirator (RIA) can be taken out of the femur with lower complications and pain in the extraction area and be combined with growth factors like bone morphogenetic proteins (BMPs), if consolidation after cancellous bone is still absent. In complex cases, consequential and radical removal of the infection often improved circulation through interventional angiography and use of the two-step procedure (the Masquelet technique) as well as a tissue covering are required. Conclusions: By using the 'diamond concept' as a complex concept, non-unions can be treated in different stages in a targeted manner.

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Incidence of donor site morbidity following harvesting from iliac crest or RIA graft

Cited by 125 publications

References 50 publications

Bone Morphogenetic Protein‐2 Promotes Human Mesenchymal Stem Cell Survival and Resultant Bone Formation When Entrapped in Photocrosslinked Alginate Hydrogels

Bone Morphogenetic Protein‐2 Promotes Human Mesenchymal Stem Cell Survival and Resultant Bone Formation When Entrapped in Photocrosslinked Alginate Hydrogels

Comparative evaluation of leukocyte- and platelet-rich plasma and pure platelet-rich plasma for cartilage regeneration

Non-Union Current Treatment Concept

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