Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia

Sani, Norrakiah Abdullah; Ghassem, Masomeh; Babji, Abdul Salam; Kupusamy, Uma Priya; I., None Jaafar, N. A.

doi:10.17576/jsm-2014-4312-06

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…In the present study, K. pneumoniae was isolated from one sample of PIF (2.7%), cereal baby food 1 (6.25%), and growing-up formula (fortified milk) 1 (5.5%). These results were nearly similar to those reported by Yao et al [30] (4.3%), Sani and Lim [31] (6.6%, (7.14%), Oonaka et al [32] (4.6%), and Estuningsih et al [33] (4%) (Table-1). However, the results of our study are not consistent with those obtained by Zhou et al [34] (26.7%), Sani and Lim [31] (6.6%), and Muytjens et al [35] (9.2%), respectively.…”

Section: Discussionsupporting

confidence: 90%

Antimicrobial susceptibility profile of Klebsiella pneumoniae isolated from some dairy products in Libya as a foodborne pathogen

Azwai,

Lawila,

Eshamah

et al. 2024

Vet World

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Background and Aim: Klebsiella pneumoniae is one of the most common causes of clinical and asymptomatic mastitis in dairy cattle, as well as in milk and dairy products that affect milk quality. Mastitis caused by K. pneumoniae is even more serious due to its poor response to antibiotic therapy. The aim of this study was to detect and identify the presence of K. pneumoniae in milk and dairy products produced in Libya. Materials and Methods: A total of 234 samples were randomly collected from various locations in Libya. Samples were examined for the presence of K. pneumoniae using conventional cultural techniques, including cultivation in violet red bile agar plus 4-methylumbelliferyl-ß-D-glucuronide broth and CHROM agar, followed by polymerase chain reaction identification and partial sequencing of 16S rRNA. Results: Of the 234 samples of milk and dairy products collected, 16 (6.8%) isolates revealed mucoid colonies on agar media that were phenotypically suggested to be K. pneumoniae. Identification of isolates was confirmed using molecular techniques (16S rRNA). Among the examined samples, K. pneumoniae was recovered from camel’s milk, raw cow’s milk, raw fermented milk, Maasora cheese, Ricotta cheese, soft cheese, full cream milk powder, milk powder infant formula, cereal baby food, and growing-up formula. Antibiotic susceptibility testing was performed on 12 of the 16 K. pneumoniae isolates, and the results showed that K. pneumoniae isolates were resistant to more than eight antibiotics; interestingly, two isolates showed metallo-beta-lactamase (MBL) production. Conclusion: K. pneumoniae is considered a risk to human health because many of these products do not comply with the microbiological criteria of international and/or Libyan standards. This study emphasized the relationship between K. pneumoniae and raw milk, cheese, milk powder, and infant milk retailed in Libya. There is a need to take the necessary measures to ensure effective hygiene practices during production in dairy factories, handling, and distribution on the market, in particular at a small local production scale. Keywords: antibiotic sensitivity, dairy products, foodborne, Klebsiella pneumoniae, Libya.

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Section: Discussionsupporting

confidence: 90%

Antimicrobial susceptibility profile of Klebsiella pneumoniae isolated from some dairy products in Libya as a foodborne pathogen

Azwai,

Lawila,

Eshamah

et al. 2024

Vet World

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“…In this study, K. pneumoniae was isolated from one sample of powder infant formula (PIF) (2.7%), cereal baby food 1(6.25%) and growing up formula (fortified milk) 1 (5.5%), these results were nearly similar to those reported by Yao et al [30] (4.3%), Sani and Lim [31] (6.6%, (7.14%), Oonaka et al [32] (4.6%) and Estuningsih et al [33] (4%) Table (1). On other hand, the results of our study are not consistent with that obtained by Zhou et al [34] (26.7%) and Sani and Lim [31] (6.6%) Muytjens et al [35] (9.2%) respectively.…”

Section: Discussionsupporting

confidence: 86%

“…Sani and Lim [31] found that K. pneumoniae was detected in one normal infant formula (6.25%) and one special infant formula milk (7.14%) In contrast, no K. pneumoniae was recovered from ready to feed baby milk (0%). Interestingly, Pereira and Vanetti [20] detected K. pneumoniae in milkbased and food supplement (20%) (Modular Enteral Diets) used in two public hospitals in Brazil.…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial Susceptibility Profile of <em>Klebsiella pneumoniae</em> Isolated as a Foodborne Pathogen From Some Dairy Products in Libya

Azwai,

Lawila,

Eshamah

et al. 2023

Preprint

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Klebsiella pneumoniae is one of the most common causes of clinical and asymptomatic mastitis in dairy cattle, also in milk and dairy products affecting its quality. Mastitis caused by K. pneumoniae is even more serious due to its poor response to antibiotic therapy. This study was conducted to detect and identify the presence of K. pneumoniae in milk and dairy products. A total of 234 samples were randomly collected from various locations in Libya. Samples were examined for the presence of K. pneumoniae by conventional cultural techniques that included cultivation in violet red bile agar plus 4-methylumbelliferyl-ß-D-glucuronide (VRBA + MUG) broth, and CHROM agar followed by identification by PCR and partial sequencing of 16S rRNA. Out of the 234 samples of milk and dairy products collected, 16 (6.8%) of the isolates revealed mucoid colonies on agar media phenotypically suggested to be K. pneumoniae. The isolates identification was confirmed by molecular techniques (16S rRNA). Among examined samples, K. pneumoniae was recovered from she camel’s milk, raw cow’s milk, raw fermented milk, Maasora cheese, Ricotta cheese, soft cheese, full cream milk powder, milk powder infant formula, cereal baby food and growing up formula. From the 16 K. pneumoniae isolates; antibiotic susceptibility testing was performed on 12 isolates, the results showed that K. pneumoniae isolates were resistant to more than 8 antibiotics, interestingly, two isolates revealed MBL production. This study emphasized the relationship between K. pneumoniae and raw milk, cheese, milk powder and infant milk retailed in Libya, which is considered as a risk for human health as many of these products did not comply with microbiological criteria of international and/or Libyan standards. The necessary precautions have to be taken to carry out effective sanitary practices during the production in dairy plants, handling and distribution in the markets particularly at local small manufacture scale.

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“…Contamination of PIF can occur at manufacturing facilities, during PIF reconstitution with contaminated water, or during inappropriate storage of reconstituted milk (Kalyantanda et al, 2015). Cronobacter sakazakii also has been found in PIF with 12.5% prevalence in 72 PIF samples in Malaysia (Norrakiah et al, 2014). C. sakazakii has been linked to life-threatening infections in infants such as sepsis, necrotizing enterocolitis (NEC), and meningitis (FAO/ WHO, 2008;Chenu & Cox, 2009;Hunter & Bean, 2013;Holy & Forsythe, 2014).…”

Section: Powdered Infant Formulamentioning

confidence: 99%

Identification of Sensitive Raw Materials at A Milk Preparation Room of A Neonatal Intensive Care Unit in the Canselor Tuanku Muhriz Hospital

Mohamad

Ishak²,

Sani

2023

MAB

Self Cite

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The implementation of a Hazard Analysis and Critical Control Point (HACCP) system in infant milk preparation can prevent and reduce the risk of bacterial contamination in infant feedings. Therefore, it is very important to determine the sensitive raw materials (SRM) in a milk room of a neonatal intensive care unit (NICU) to prevent contamination that can affect the safety of milk feeding. This study was conducted to identify the SRM at the milk preparation room of a NICU in the Canselor Tuanku Muhriz Hospital, Cheras, Kuala Lumpur, Malaysia. SRM were identified via HACCP system decision tree by the Malaysian Standard (MS1480:2007).. The results showed that donor expressed breast milk (DEBM), expressed breast milk at home (EBMH), freshly expressed breast milk (FEBM) and powdered infant formula (PIF) were identified as SRM. Expressed breast milk (DEBM, EBMH, FEBM) were known as non-sterile milk which were able to transmit pathogenic microorganisms such as Klebsiella pneumoniae and Pseudomonas aeruginosa derived from the mother or donor. PIF is also not sterile that are associated with Cronobacter sakazakii and Salmonella infections causing serious illnesses among premature and infants. Ready to feed milk (RTF) is classified as non-sensitive material as it is sterile, free from bacterial contamination and safe for high-risk infant’s feeding compare to PIF. Preventive measures were applied to control the significant hazards in all identified SRM to ensure the final product (milk) is safe for consumption.

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Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia

Cited by 7 publications

References 27 publications

Antimicrobial susceptibility profile of Klebsiella pneumoniae isolated from some dairy products in Libya as a foodborne pathogen

Antimicrobial susceptibility profile of Klebsiella pneumoniae isolated from some dairy products in Libya as a foodborne pathogen

Antimicrobial Susceptibility Profile of <em>Klebsiella pneumoniae</em> Isolated as a Foodborne Pathogen From Some Dairy Products in Libya

Identification of Sensitive Raw Materials at A Milk Preparation Room of A Neonatal Intensive Care Unit in the Canselor Tuanku Muhriz Hospital

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