Incidence of class 1 integrons in multiple antibiotic-resistant Gram-negative copiotrophic bacteria from the River Torsa in India

Mukherjee, S.; Chakraborty, Ranadhir

doi:10.1016/j.resmic.2005.08.003

Cited by 67 publications

(35 citation statements)

References 31 publications

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“…The primer Int2F, which is specific to the 39 region of the integrase gene (with~600 bp upstream from the 59-CS primer site), was used in combination with the 39-CS primer to show the proximity of the inserted gene cassettes to integrons (Mukherjee & Chakraborty, 2006). PCR-RFLP using HinfI enzyme was performed to determine whether the different isolates carried identical gene cassettes.…”

Section: Methodsmentioning

confidence: 99%

New integron gene arrays from multiresistant clinical isolates of members of the Enterobacteriaceae and Pseudomonas aeruginosa from hospitals in Malaysia

Kor

Choo

Chew

2013

Journal of Medical Microbiology

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This study investigated 147 multidrug-resistant Enterobacteriaceae and Pseudomonas aeruginosa isolates from hospitalized patients in Malaysia. Class 1 integrons were the most dominant class identified (45.6 %). Three isolates were shown to contain class 2 integrons (2.0 %), whilst one isolate harboured both class 1 and 2 integrons. No class 3 integrons were detected in this study. In addition, the sul1 gene was amplified in 35 % of isolates and was significantly associated with the presence of integrase genes in an integron structure. RFLP and DNA sequencing analyses revealed the presence of 19 different cassette arrays among the detected integrons. The most common gene cassettes were those encoding resistance towards aminoglycosides (aad) and trimethoprim (dfr). As far as is known, this study is the first to identify integron-carrying cassette arrays such as aadA2-linF, aacC3-cmlA5 and aacA4-catB8-aadA1 in the Malaysian population. Patients' age was demonstrated as a significant risk factor for the acquisition of integrons (P50.028). Epidemiological typing using PFGE also demonstrated a clonal relationship among isolates carrying identical gene cassettes in Klebsiella pneumoniae and P. aeruginosa but not in Escherichia coli isolates.

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Section: Methodsmentioning

confidence: 99%

New integron gene arrays from multiresistant clinical isolates of members of the Enterobacteriaceae and Pseudomonas aeruginosa from hospitals in Malaysia

Kor

Choo

Chew

2013

Journal of Medical Microbiology

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“…Among them, class 1 integron is most prevalent in water environments. Class 1 integronase gene (intI1) has often been shown to be carried by bacterial strains isolated from animal production or aquaculture facilities (Schmidt et al 2001;Moura et al 2007;Akinbowale et al 2007;Jacobs and Chenia 2007), STPs (Szczepanowski et al 2004;Taviani et al 2008), surface water (Mukherjee and Chakraborty 2006) and sediments (Dalsgaard et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Class 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China

Zhang

et al. 2009

Ecotoxicology

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Class 1 integronase gene (intI1) and tetracycline resistance genes (tetA and tetC) from various environmental sites in Jiangsu Province (China) were detected using qualitative PCR (polymerase chain reaction) and quantified with SYBR Green-based qRT-PCR (quantitative real-time PCR) in this study. Qualitative PCR assays demonstrated that intI1, tetA and tetC occurred in the water environments of Taihu Lake, the Nanjing section of the Yangtze River, a sewage treatment plant (STP) in Nanjing City, and two drinking water treating bioreactors. qRT-PCR results showed that abundance of intI1 in lake water and sediments was lower than the tet genes, for a given sample site and date (P < 0.05). On a volumetric basis, lake sediments contained higher concentrations of the three genes by four to five orders of magnitude than water samples, and lake water and sediments sampled in April contained fewer copies of all the genes than the samples collected in June and August (P < 0.05). The levels of intI1, tetA and tetC in the Yangtze River water increased significantly after the river flowed through Nanjing City (P < 0.05). 94.1% integron, 97.2% tetA and 98.3% tetC were removed by the activated sludge process in the STP, and more than 80% of each gene was removed in both of the two biofilters in terms of relative concentration based on sample volume. However, on the basis of DNA mass, lower removals were obtained for both the activated sludge and biofiltration processes.

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“…Resistance genetic determinants have also been detected in a large number of microbial communities from natural environments (Alonso et al 2001). Antibiotic resistance in Enterobacteriaceae, partially bacterial opportunist pathogens, has increased dramatically in aquatic environments (Schwartz et al 2003;Henriques et al 2006;Mukherjee and Chakraborty 2006). The prevalence of diverse antibiotic-resistant bacteria and resistance genes makes coastal ecosystems a reservoir of antibiotic resistance (Young 1993; Biyela et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China

Wang

Dang

Ding

2008

World J Microbiol Biotechnol

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Environmental microbiology investigation was performed to determine the molecular diversity of b-lactamase genes among ampicillin-resistant bacteria from Jiaozhou Bay. b-lactamase genes were detected in 93.8% of the bacterial isolates identified as Enterobacteriaceae. The most frequently detected gene was bla TEM , followed by bla SHV , bla OAX-1 , bla MOX and bla CMY . Most of the isolates (68.8%) were positive for the intI1 integrase gene, and two isolates were also found for the intI2 gene. The dfr and aadA gene cassettes were predominant. Anthropogenic contamination from onshore sewage processing plants might contribute predominantly to the b-lactamase gene reservoir in the studied coastal waters. Environmental antibiotic-resistant bacteria and resistance genes may serve as bioindicators of coastal environmental quality or biotracers of the potential contamination sources. This is the first report of the prevalence and characterization of blactamase genes and integrons in coastal Enterobacteriaceae from China.

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Incidence of class 1 integrons in multiple antibiotic-resistant Gram-negative copiotrophic bacteria from the River Torsa in India

Cited by 67 publications

References 31 publications

New integron gene arrays from multiresistant clinical isolates of members of the Enterobacteriaceae and Pseudomonas aeruginosa from hospitals in Malaysia

New integron gene arrays from multiresistant clinical isolates of members of the Enterobacteriaceae and Pseudomonas aeruginosa from hospitals in Malaysia

Class 1 integronase gene and tetracycline resistance genes tetA and tetC in different water environments of Jiangsu Province, China

Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China

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