Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue: an interphase cytogenetic study1

Correlation of cytologic findings and chromosomal instability detected by fluorescence in situ hybridization in breast fine-needle aspiration specimens from women at high risk for breast cancer

“…Highrisk patients had significantly more monosomy in chromosomes 1, 11, and 17 and polysomy in chromosome 8 compared to patients at low risk. These findings are in agreement with findings reported previously [9][10][11] and suggest that detection of aneusomy may be a useful marker for increased risk of breast cancer development. In our series, the number of chromosomes with polysomy increased as atypia progressed.…”

“…The lack of correlation between aneusomy and cell morphology may be partly due to the use of a separate slide preparation for FISH analysis, which prevented direct correlation of aneusomy with cell morphology, or to the fact that high-risk patients harbor chromosomal aberrations irrespective of cell morphology. 11 Previous studies suggest that certain patterns of aneusomy are indicative of malignancy. 16,21 In a study by Fehm et al, 21 several aneusomic patterns in , and polysomy for chromosome 1 (31% of cases).…”

“…1,5 Consequently, ancillary studies to better assess cellular changes are being sought. 6 Breast cancer progression is characterized by the accumulation of numeric changes on many chromosomes, particularly early or frequent increases in the copy numbers of chromosomes 1,8,11, and 17. [7][8][9][10][11][12][13][14][15][16] Fluorescence in situ hybridization (FISH) evaluation of chromosomal aberrations in ductal lavage specimens may be more sensitive and specific than conventional cytologic evaluation for categorization of breast lesions.…”

“…[7][8][9][10][11][12][13][14][15][16] Fluorescence in situ hybridization (FISH) evaluation of chromosomal aberrations in ductal lavage specimens may be more sensitive and specific than conventional cytologic evaluation for categorization of breast lesions. [17][18][19] To determine the utility of FISH in categorizing breast epithelial changes, we used this technique to evaluate numeric changes in chromosomes 1,8,11, and 17 in breast FNA specimens from 27 women at high risk for breast cancer. For comparison, we used cytologic smears from seven invasive ductal breast carcinomas and nine specimens of nonproliferative epithelium (NPE) from women at low risk for breast cancer.…”

Aneusomy 17 in Breast Cancer: Its Role in HER-2/neu Protein Expression and Implication for Clinical Assessment of HER-2/neu Status

“…Although HER-2/neu protein overexpression in the presence of the specific gene amplification is presumably through the increased gene dosage, such gene dosage increase may also conceivably occur because of a nonspecific increase in chromosome 17 copy number per cell (polysomy 17). Indeed, a significant number of breast cancer specimens have shown aneusomy 17, i.e., deviation from the normal state of disomy 17 (two copies of chromosome 17 per cell), with either more (polysomy) or fewer (hypodisomy) copies (12)(13)(14). Such findings have raised an important question, i.e., what role, if any, the chromosome 17 copy number plays in HER-/neu gene dosage and protein overexpression.…”