Korean Native cattle (Hanwoo) is the most important breed as an economically important livestock in Republic of Korea (Korea). The reproduction of Hanwoo calves plays a significant role in overall productivity of the cattle industry. Abortion in cattle defined as the loss of a fetus, is a primary cause of reproductive losses and carries economic importance in cattle (Cabell, 2007; Hovingh et al, 2009). Most diagnosed abortions in cattle are caused by infections of bacterial, viral, fungal and protozoal agents (Anderson, 2007). Especially, brucellosis (caused by Brucella abortus) and bovine tuberculosis (BTB, caused by Mycobacterium bovis) can have a pro-found impact on the fertility of cattle population (Joly and Messier, 2005). Brucellosis, one of the most important zoonotic diseases, is caused by bacteria belonging to the genus Brucella, a Gram-negative bacterium (Cutler et al, 2005). In cattle, brucellosis is caused by B. abortus and it has a great economic implication due to reproductive failures, including abortion during the last trimester of gestation, infertility, orchitis, and epididymitis in males (Godfroid et al, 2005; Jung et al, 2010; Kaaboub et al, 2019). For decades, bovine brucellosis has been recognized as one of the most important endemic animal diseases in numerous countries, including Korea (Yoon et al, 2014).