Incidence of blaNDM-1 gene in Escherichia coli isolates at a tertiary care referral hospital in Northeast India

Bora, Arijit; Ahmed, GU; Hazarika, Naba Kumar; Prasad, K. N.; Shukla, Sanket K.; Randhawa, Vinay; Sarma, Jogesh

doi:10.4103/0255-0857.115628

Cited by 27 publications

(18 citation statements)

References 33 publications

(33 reference statements)

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“…In the present study, CRE isolates exhibited increased prevalence of multidrug resistance to various antibiotics ranging from 66.6% to 100% which is comparable to a study from Northeast India with resistance rate ranging from 85.7% to 100% 14 .…”

Section: Discussionsupporting

confidence: 82%

Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup

Sokhi¹,

Agarwal²,

Maheshwari³

et al. 2020

J. Pure Appl. Microbiol.

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Carbapenem resistance is increasing and emerging as a public health threat. The Indian subcontinent serves as a reservoir for all 3 types of carbapenemases: KPC, OXA-181 and NDM. The present study was done to determine the antibiotic resistance pattern of Gram negative bacilli (GNB) belonging to family Enterobacteriaceae and molecular detection of bla KPC gene among the Carbapenem resistant Enterobacteriaceae (CRE). Antibiotic sensitivity pattern of 301 gram negative isolates, members of Enterobacteriaceae (E. coli, K. pneumoniae, K. oxytoca, Proteus species, Citrobacter species, Enterobacter species and Serratia species) was determined and those resistant to carbapenem (ertapenem) were further processed in the study. Modified Hodge Test (MHT) was performed to phenotypically confirm the presence of carbapenemases (bla KPC) and Polymerase Chain Reaction (PCR) was done to identify bla KPC gene. Of the total 301 isolates, 45 were resistant to ertapenem. Out of these 45 isolates 32 were MHT positive and three of these 32 MHT positive strains harboured bla KPC gene.

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Section: Discussionsupporting

confidence: 82%

Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup

Sokhi¹,

Agarwal²,

Maheshwari³

et al. 2020

J. Pure Appl. Microbiol.

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“…MBL producing bacterial isolates can confer resistance to carbapenems and all beta-lactam agents except aztreonam although coexistence of other resistance mechanisms such as AmpC type beta-lactamases or ESBLs render them resistant to aztreonam [20]. Likewise, all the isolates of E. coli and K. pneumoniae with MBL production in the present study were found to be resistant to all three carbapenems (imipenem, meropenem and ertapenem).…”

Section: Discussionmentioning

confidence: 46%

“…In this study, out of 80 E. coli and K. pneumoniae isolates initially screened for carbapenemase production, 73 (91.25%) isolates exhibited fairly positive results in MHT, whereas 7 (8.75%) isolates exhibited weakly positive results in MHT. Different studies also reported the occurrence of weakly positive results for the MHT in the detection of MBL producing Enterobacteriaceae [19, 20]. …”

Section: Discussionmentioning

confidence: 99%

Incidence of metallo-beta-lactamase producing clinical isolates of Escherichia coli and Klebsiella pneumoniae in central Nepal

et al. 2014

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BackgroundThe increasing and rapid spread of metallo-beta-lactamase (MBL) producing Enterobacteriaceae, particularly Escherichia coli and Klebsiella pneumoniae represents an emerging public health threat. However, limited data is available on MBL production in clinical isolates of E. coli and K. pneumoniae from Nepal. We have therefore undertaken this study to ascertain the incidence of MBL production in clinical isolates of E. coli and K. pneumoniae at a tertiary care teaching hospital in central Nepal.MethodsA total of 401 consecutive, non-duplicate isolates of E. coli (n = 216) and K. pneumoniae (n = 185) were recovered from various clinical samples between July and December, 2012. These isolates were screened for the detection of carbapenemase production on the basis of their reduced susceptibility to meropenem or ertapenem by the disc diffusion method. The screened isolates were further phenotypically studied for carbapenemase production by modified Hodge test (MHT). MBL production was detected by performing combined disc test by using imipenem discs with and without ethylenediaminetetraacetic acid (EDTA), which chelates zinc required for MBL activity.ResultsOut of 216 E. coli isolates, a total of 41 isolates (18.98%) and out of 185 K. pneumoniae isolates, a total of 39 isolates (21.08%) were suspected to be carbapenemase- producers on the basis of their reduced susceptibility to meropenem or ertapenem. Interestingly, all the initially suspected isolates of E. coli and K. pneumoniae for carbapenemase production were found to be positive in both MHT and combined disc test. However, few weakly positive reactions were observed in MHT. All the MBL producing isolates were multidrug-resistant (MDR). In addition, 75.60% E. coli and 71.79% of K. pneumoniae isolates producing MBL were found to be “pandrug- resistant”.ConclusionsOur findings showed MBL production in a considerable number of E. coli and K. pneumoniae isolates with MDR and pandrug-resistant phenotypes. Combined disc method can provide a sensible choice for phenotypic detection of MBL production in clinical microbiology laboratories as detection of MBL in bacterial isolates is indispensable for establishing the effective antibiotic policies and infection control strategies in the hospital setting.

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“…This is also the case in the Indian subcontinent, where the emergence of a new metallo-β-lactamase (MBL) gene-the blaNDM-1, was reported recently [28] [29]. The subsequent worldwide reports on the emergence of blaNDM-1 producing bacteria clearly showed the role of plasmid vectors in their dissemination as they were found to be residents on large plasmids.…”

Section: Discussionmentioning

confidence: 85%

Detection of blaNDM-1 and Genetic Relatedness in Clinical Isolates of Escherichia coli Producing Extended Spectrum β-Lactamase from Tertiary Care Centres in South India

Narayanan¹,

Suresh²,

Rajamma³

et al. 2016

AiM

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Background: Extended spectrum β-lactamases (ESBL) producing E. coli co-producing other β-lactamases and exhibiting co-resistance to different antibiotic classes continue to emerge as a threat to clinical field. This study aimed to analyze the co-production of New Delhi metallo-β-lactamase-1 (blaNDM-1) in ESBL producing plasmid-bearing clinical isolates collected from two tertiary care centres in Kerala, South India, and to understand their genetic relatedness. Methods: Antibiotic resistance phenotypes of 44 clinical isolates were determined by disc-diffusion method. Plasmidbearing isolates, detected by the alkaline-lysis method, which also tested positive for ESBL production, were screened for the presence of blaNDM-1 by polymerase chain reaction. Plasmid, random amplified polymorphic DNA profiles and blaNDM-1 sequence-based phylogenetic tree were analyzed to understand the genotypic similarities among the isolates. Results: Beta-lactam antibiotics, quinolones, cephalosporins, used in this study, and AZM were found to be ineffective against the isolates as significantly high number of isolates were resistant to these antibiotics (P < 0.01). Plasmid bearing isolates constituted 57% (n = 25), all of which were found to be ESBL producers. blaNDM-1 amplicons were noticed in four (16%) isolates and these DNA sequences showed homology between them and with similar sequences reported from other countries like Japan and Korea. Plasmid and RAPD profiles demonstrated that most of the isolates, including those harbouring blaNDM-1 shared genetic similarities as well as an apparent geographical distinctiveness. Conclusion: The predominance of ESBL production and the occurrence of blaNDM-1 in plasmid-bearing isolates observed in our study corroborate the worldwide drug-resistance * Corresponding author.N. Narayanan et al. 126scenario. This study thus warrants the need for constant surveillance in the face of sparse information available in Kerala State on the emerging drug resistance in clinical bacteria.

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Incidence of blaNDM-1 gene in Escherichia coli isolates at a tertiary care referral hospital in Northeast India

Abstract: Our findings highlight the incidence of blaNDM-1 in E. coli isolates with a reduced susceptibility to meropenem or ertapenem.

Cited by 27 publications

References 33 publications

Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup

Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup

Incidence of metallo-beta-lactamase producing clinical isolates of Escherichia coli and Klebsiella pneumoniae in central Nepal

Detection of <i>bla</i>NDM-1 and Genetic Relatedness in Clinical Isolates of <i>Escherichia coli</i> Producing Extended Spectrum <i>β</i>-Lactamase from Tertiary Care Centres in South India

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Incidence of blaNDM-1 gene in Escherichia coli isolates at a tertiary care referral hospital in Northeast India

Abstract: Our findings highlight the incidence of blaNDM-1 in E. coli isolates with a reduced susceptibility to meropenem or ertapenem.

Cited by 27 publications

References 33 publications

Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup

Detection of Klebsiella pneumoniae Carbapenemase (KPC) Producing Enterobacteriaceae Isolates from Various Clinical Samples in a Rural Health Setup

Incidence of metallo-beta-lactamase producing clinical isolates of Escherichia coli and Klebsiella pneumoniae in central Nepal

Detection of &lt;i&gt;bla&lt;/i&gt;NDM-1 and Genetic Relatedness in Clinical Isolates of &lt;i&gt;Escherichia coli&lt;/i&gt; Producing Extended Spectrum &lt;i&gt;β&lt;/i&gt;-Lactamase from Tertiary Care Centres in South India

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Detection of <i>bla</i>NDM-1 and Genetic Relatedness in Clinical Isolates of <i>Escherichia coli</i> Producing Extended Spectrum <i>β</i>-Lactamase from Tertiary Care Centres in South India