Incidence of Active HCV infection amongst Blood Donors of Mardan District, Pakistan

Karim, Fawad; Nasar, Abu; Alam, Ibrar; Alam, Iftikhar; Hassan, Said; Gul, Rahmat; Ullah, Sana; Rizwan, Muhammad

doi:10.7314/apjcp.2016.17.1.235

Cited by 13 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the key steps involved in triggering of apoptotic cascade is the leakage of cytochrome C from the mitochondria and subsequent activation of caspases [52]. Several studies reported that phytochemical compounds of M. oleifera were able to induce apoptosis in cancer cells [27, 53, 54]. In particular, moringin, the ITC resulting from myrosinase-catalysed hydrolysis of glucomoringin (GMG-ITC) was found to induce caspase 3-dependent apoptosis in multiple myeloma [28].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Moringa isothiocyanate complexed with α-cyclodextrin: a new perspective in neuroblastoma treatment

Giacoppo

Iori

Rollin

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundSeveral lines of evidence suggest the consume of natural products for cancer prevention or treatment. In particular, isothiocyanates (ITCs) exerting anti-cancer properties, have received great interest as potential chemotherapeutic agents.This study was designed to assess the anti-proliferative activities of a new preparation of Moringa oleifera-derived 4-(α-L-rhamnopyranosyloxy)benzyl ITC (moringin) complexed with alpha-cyclodextrin (moringin + α-CD; MAC) on SH-SY5Y human neuroblastoma cells. This new formulation arises in the attempt to overcome the poor solubility and stability of moringin alone in aqueous media.MethodsSH-SY5Y cells were cultured and exposed to increasing concentrations of MAC (1.0, 2.5 and 5.0 μg). Cell proliferation was examined by MTT and cell count assays. The cytotoxic activity of the MAC complex was assessed by lactate dehydrogenase (LDH) assay and trypan blue exclusion test. In addition, western blotting analyses for the main apoptosis-related proteins were performed.ResultsTreatment of SH-SY5Y cells with the MAC complex reduced cell growth in concentration dependent manner. Specifically, MAC exhibited a potent action in inhibiting the PI3K/Akt/mTOR pathway, whose aberrant activation was found in many types of cancer. MAC was also found to induce the nuclear factor-κB (NF-κB) p65 activation by phosphorylation and its translocation into the nucleus. Moreover, treatment with MAC was able to down-regulate MAPK pathway (results focused on JNK and p38 expression). Finally, MAC was found to trigger apoptotic death pathway (based on expression levels of cleaved-caspase 3, Bax/Bcl-2 balance, p53 and p21).ConclusionThese findings suggest that use of MAC complex may open novel perspectives to improve the poor prognosis of patients with neuroblastoma.

show abstract

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Moringa isothiocyanate complexed with α-cyclodextrin: a new perspective in neuroblastoma treatment

Giacoppo

Iori

Rollin

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…The prevalence of HCV varied among population. ELISA and RT-PCR techniques were used in many cities to determine the prevalence of HCV, including 6.6% in Kohat (11,12), 9.2% in the Swat Region, 5.2% in Mardan, 9.3% in DI Khan, and 12.9% in Abbottabad (12,13). The prevalence variations may be due to a variety of factors, including differences in the number of CKD patients in each study, genetic factors, immune status of patients, detection technique type, sample size, cultural knowledge of exposure to a risk factor for HCV transmission, and the diversity of the research that characterized the virus, which can contribute to a successful age group.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Detection of Genotyping of Hepatitis C Virus in Hemodialysis Patients

Bilal,

Ali,

Farooq

et al. 2023

JHRR

View full text Add to dashboard Cite

Background: Hepatitis C virus is a significant global health distress, affecting millions of individuals worldwide. HCV is a disease that spreads through blood and mostly affects the liver. The condition may resolve on its own or progress to cirrhosis, fibrosis, or liver cancer. Chronic kidney disease patients on hemodialysis are at an increased risk for acquiring HCV infection due to cross contamination in dialysis units, blood transfusion, and kidney transplant. The aims of the study were to determine the genotypes among patients of HCV undergoing hemodialysis. Objective: The objective of the study was to assess the prevalence and types of HCV genotypes in hemodialysis patients. Methods: In this cross-sectional study, the HCV positive patients undergoing hemodialysis were enrolled. Samples were collected from the dialysis unit of Farooq Hospital Lahore. RT-PCR was performed to determine the HCV genotypes. This study was conducted on patients of both genders and all age groups. Chi-square test was performed to assess any significant correlation between HCV genotypes in all age groups and both genders. Results: In this study out of a total of 80 hemodialysis patients, 19 (23.8%) were females and 61 (76.3%) were males. The most common genotype observed was 3 (72.5%) followed by 1a (12.5%), 1(10.0%) and 1b (5.0%). Conclusion: The current study revealed that the genotype 3 of HCV as the most frequent genotypes in the hemodialysis patients of our population with most patients in the age group of <60 years.

show abstract

“…Persistence of HCV is not commonly high among individuals with antibodies, and this finding was unusual. There is no other mention to such a high prevalence of virus persistence [33,34]. …”

Section: Discussionmentioning

confidence: 99%

Detection of HCV Persistent Infections in the Dental Pulp: A Novel Approach for the Detection of Past and Ancient Infections

Siravenha

Madeira

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

The dental pulp is a sterile highly vascularized tissue and has been commonly used as a biological material to detect the genome of infectious agents that reach the dental tissue. Indeed, the pulp is also used to reveal past and ancient infections in the field of paleomicrobiology. The present study aimed to detect the presence of Hepatitis C virus (HCV) in a small community (approximately 400 inhabitants) in the Amazon region of Brazil (Nossa Senhora do Perpetuo Socorro, Vizeu, Para, Brazil) and standardize a technique for the detection of the virus in the dental pulp. Serum samples were collected from 48 patients whose teeth were clinically recommended for surgical extraction. The group comprised an equal number of males and females, mostly agriculture workers and housewives, respectively. The majority (64.6%) received less than one minimum wage and were ill educated (less than four years of school years). An enzyme immune assay was used to detect antibodies to HCV and the 9 (18.8%) positive samples were submitted to nucleic acid extraction in the blood (using the EXTRAzol) and the pulp (QIAamp DNA Micro Kit e kit RNeasy Plus Micro). The pulp was removed using a modified protocol without the use of liquid nitrogen. Nucleic acid was found in 8 of the dental pulp, but in 7 of the blood samples. Sequencing of one of the samples showed the presence of genotype 1. Conclusions: A novel simplified methodology for the extraction and amplification of HCV nucleic acid was successful to detect the presence of persistent infections of the virus within the dental pulp tissue. The protocol may be helpful to detect past and ancient infections and to better understand the natural history of HCV.

show abstract

Incidence of Active HCV infection amongst Blood Donors of Mardan District, Pakistan

Cited by 13 publications

References 15 publications

RETRACTED ARTICLE: Moringa isothiocyanate complexed with α-cyclodextrin: a new perspective in neuroblastoma treatment

RETRACTED ARTICLE: Moringa isothiocyanate complexed with α-cyclodextrin: a new perspective in neuroblastoma treatment

Prevalence and Detection of Genotyping of Hepatitis C Virus in Hemodialysis Patients

Detection of HCV Persistent Infections in the Dental Pulp: A Novel Approach for the Detection of Past and Ancient Infections

Contact Info

Product

Resources

About