Incidence and Diversity of Double-Stranded RNA in Lentinula edodes

Rytter, J. L.; Royse, D. J.; Romaine, C. P.

doi:10.2307/3760362

Cited by 5 publications

(7 citation statements)

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“…It is uncertain whether mycoviruses of mushrooms are the immediate cause of abnormalities because they are occasionally detected in asymptomatic mycelia and fruiting bodies. Furthermore, whether the presence of a specific mycovirus is closely associated with the vegetative characteristics of mycelia and formation of fruiting bodies has been investigated [ 30 ]. Preparation of virus-free isogenic lines is essential to verifing fungal symptoms of PoV-ASI2792 infection by comparison of isogenic virus-free P .…”

Section: Resultsmentioning

confidence: 99%

“…Viral infection of P. otreatus are also revealed reduced mycelial growth, delayed fruiting body formation, decreased fruiting body yield, and malformed fruiting bodies [ 5 8 9 10 12 ]. However, virus-free and virus-infected isogenic lines, which have identical genetic backgrounds, are needed to investigation of mycovirus-fungal host interactions because noncoincidence genetic backgrounds of fungal strains can lead to different symptoms in response to infection with the same mycovirus [ 19 30 ]. According to pair-wise comparison of isogenic strains, we sought to preferable determine the interaction between PoV-ASI2792 infection and vegetative characteristics of P. ostreatus .…”

Section: Resultsmentioning

confidence: 99%

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Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus

et al. 2016

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A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the P. ostreatus ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in the P. ostreatus strain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-free P. ostreatus strains with a virus-infected strain, isogenic virus-cured P. ostreatus strains were obtained by the mycelial fragmentation method for virus curing. The absence of virus was verified with gel electrophoresis after dsRNA-specific virus purification and Northern blot analysis using a partial RdRp cDNA of PoV-ASI2792. The growth rate and mycelial dry weight of virus-infected P. ostreatus strain with PoV-ASI2792 mycovirus were compared to those of three virus-free isogenic strains on 10 different media. The virus-cured strains showed distinctly higher mycelial growth rates and dry weights on all kinds of experimental culture media, with at least a 2.2-fold higher mycelial growth rate on mushroom complete media (MCM) and Hamada media, and a 2.7-fold higher mycelial dry weight on MCM and yeastmalt-glucose agar media than those of the virus-infected strain. These results suggest that the infection of PoV mycovirus has a deleterious effect on the vegetative growth of P. ostreatus.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus

et al. 2016

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“…Besides, this virus were mostly found to localize in the cytoplasm and in the vacuoles of hyphal cells, either with randomly dispersed or with aggregated forms [86]. In a study on determining the prevalence and diversity of dsRNA elements in L. edodes strains present in the US, 23 out of 25 genetically-diverse lines of L. edodes were shown to harbor dsRNA molecules with total of 14 different molecular weights [71]. However, the presence or absence of particular dsRNA element could not be correlated with any certain phenotypic characterictic and there were also no evidence of harmful effects associated with the presence of dsRNA elements in the studied L. edodes strains [71].…”

Section: Viruses Of Lentinula Edodesmentioning

confidence: 99%

Viruses infecting macrofungi

Şahin

Akata

2018

VirusDis.

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Ever since their discovery just about 56 years ago in the cultivated mushroom , many more viruses infecting fungi have been identified in a wide range of fungal taxa. With mostly being asymptomatic, especially the ones that are detrimental to their phytopathogenic hosts are intensively studied due to their considerable importance in developing novel plant protection measures. Contrary to the rapid accumulation of notable data on viruses of plant pathogenic microfungi, much less information have hitherto been obtained in regards to the viruses whose hosts are macrofungi. According to the current literature, only more than 80 distinct viruses bearing either linear dsRNA or linear positive sense ssRNA genome and infecting a total number of 34 macrofungal species represented with four and 30 have been identified so far. Among these 34 macrofungal species, 14 are cultivated edible and wild edible mushroom species. According to the 10th ICTV (International Committee on Taxonomy of Viruses) Report, macrofungal viruses with linear dsRNA genome are classified into five families (, ,, and) and macrofungal viruses with linear positive sense ssRNA genome are classified into seven families (, ,, ,, and). In this review, following a brief overview of some general characteristics of fungal viruses, an up to date knowledge on viruses infecting macrofungal hosts were presented by summarizing the previous, recent and prospective studies of the field.

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“…Several reports on the discovery of mycovirus in L. edodes exist Magae, 2012;Rytter et al, 1991;Won et al, 2013). However, because these mycoviruses are commonly found in healthy and asymptomatic fruiting bodies, it is unclear whether they are the direct cause of abnormalities (Rytter et al, 1991). In addition, it has not been determined if a relationship exists between the presence or absence of a particular mycovirus and the vegetative characteristics of mycelia.…”

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confidence: 98%

“…This makes it difficult to determine whether the changes in phenotypes are due to the mycovirus, other cytological inheritance, or both (Nuss, 2005 in terms of worldwide production and economic value (Hadeler, 1995) and is one of two most important commercial mushrooms in Korea (Ko et al, 2012). Several reports on the discovery of mycovirus in L. edodes exist Magae, 2012;Rytter et al, 1991;Won et al, 2013). However, because these mycoviruses are commonly found in healthy and asymptomatic fruiting bodies, it is unclear whether they are the direct cause of abnormalities (Rytter et al, 1991).…”

mentioning

confidence: 99%