Incidence and Distribution of <i>Puccinia coronata</i> and <i>P. graminis</i> on Turfgrass in the Midwestern United States

Karakkat, Brijesh B.; Jackson, Vonte; Koch, Paul L.

doi:10.1094/pdis-09-17-1353-re

Cited by 3 publications

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Puccinia striiformis Westend (stripe rust), Puccinia recondita Roberge ex Desmaz. and Puccinia brachypodii G. Otth (leaf rusts) are considered the most prevalent rust pathogens of turfgrass (Karakkat et al 2018, Smiley et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Türkiye'deki çim alanlarında Puccinia spp.nin qPCR analizi

ÜNAL,

TÜLEK

2023

Bitki Koruma Bülteni

View full text Add to dashboard Cite

In recent years, with the increase in parks, gardens, recreation areas, picnic areas and refuges with urbanization, the interest in turfgrass areas that beautify the aesthetic appearance of these areas has also increased. In these areas, rust diseases are also becoming increasingly common and causing problems. Rust diseases are an important pathogen group that needs to be monitored due to their ability to create new races and their airborne transmission. In this study, surveys were conducted in turfgrass areas in 8 provinces of Türkiye and 110 turfgrass leaf samples showing rust symptoms were collected. As a result of DNA isolation and qPCR analysis from pustules on the leaf surface, 37 P. coronata, 32 Puccinia graminis, 18 P. striiformis and 23 Puccinia spp. have been detected. It was determined that the most common rust species that causes disease in turfgrass areas in Türkiye is Puccinia coronata. This species was followed by P. graminis and P. striiformis, respectively. While P. striiformis was mostly isolated from Kayseri and Istanbul Province, P. coronata and P. graminis were mostly isolated from İstanbul.

show abstract

Section: Introductionmentioning

confidence: 99%

Türkiye'deki çim alanlarında Puccinia spp.nin qPCR analizi

ÜNAL,

TÜLEK

2023

Bitki Koruma Bülteni

View full text Add to dashboard Cite

show abstract

The U.S. Capitol Grounds and Arboretum: an iconic landscape with a holistic approach to pest management

Mueller,

Naas,

Serock

et al. 2024

Journal of Integrated Pest Management

View full text Add to dashboard Cite

The care and maintenance of managed urban greenspaces require integrated pest management (IPM) strategies that are best suited for their local conditions. The evolution of IPM for the public grounds of the U.S. Capitol and Congress provides a unique perspective into the distinct challenges in developing and implementing an overall plant healthcare program for the nation’s historical landscape. The Capitol Grounds and Arboretum (CGA) staff of the Architect of the Capitol are the stewards of this urban greenspace, located in the center of Washington D.C., and present an overview of their historical, current, and prospective IPM developments for urban landscape pest control. With the adoption of holistic, ecological-based pest management practices, successful IPM examples in 3 different landscape habitats on Capitol Hill—turfgrass lawns, urban forestry, and ornamental horticulture display beds—are described. Ultimately, a list of current programming and future opportunities for expanding ecological IPM practices best suited for solving the CGA’s unique landscape pest management challenges are summarized.

show abstract

First Report of Stem Rust, Caused by Puccinia graminis, and Crown Rust, Caused by Puccinia coronata var. avenae f. sp. avenae, on Tall Fescue (Festuca arundinacea) in Georgia, U.S.A.

et al. 2021

View full text Add to dashboard Cite

Stem rust, caused by Puccinia graminis, and crown rust, caused by P. coronata, are common rust diseases on cool-season grasses (Karakkat et al. 2018), for which long-distance spore dispersal was recorded in northern US (Harder and Haber 1992). During the summers of 2019 and 2020, severe infection of stem rust and crown rust was observed on > 60% of tall fescue (Festuca arundinacea) germplasm plants in a breeding nursery located at the University of Georgia, Griffin GA. Rust-infected leaves first presented uredinia pustules, then black telia towards the end of the season. The uredinia pustules of stem rust and crown rust were brick-red and, yellow and arranged along the host veins, respectively. The urediniospores were one-celled, round to ovoid and measured from 20.75±2.44 μm (crown rust) to 27±3.60 μm long (stem rust). The teliospores were two-celled and measured from 45.75±10.14 μm (stem rust) to 51.60±4.0 μm long (crown rust) (Leonard et al. 2005; Cummins 1971). Urediniospores of both rusts were collected from infected plants in the field in April of 2020 using a Piston vacuum pump (Welch by Gardner Denver Ltd.) and stored at -80 °C in 1.5 ml Eppendorf tubes. Genomic DNA was extracted by grinding the urediniospores in liquid nitrogen using mortar and pestle, followed by the cetyltrimethylammonium bromide method (Doyle and Doyle 1987). The internal transcribed spacer (ITS) region of the ribosomal DNA was amplified using the ITS5-ITS4 primers (White et al. 1990). BLASTn and phylogenetic analyses revealed that the sequence of stem rust (GenBank acc. no. MW430963) and crown rust (GenBank acc. no. MW431324) pathogens had >99% similarity with P. graminis (GenBank acc. no. HQ317538) and P. coronata var. avenae f. sp. avenae (clade V; Liu and Hambleton 2013) (GenBank acc. no. EU014044), respectively. Pathogenicity tests were conducted on the tall fescue cultivar ‘Bandit’. For each rust, 12 pots (10 cm × 10 cm) were planted, each containing 13 seeds in a Sungro professional growing mix soil (Sun Gro Horticulture Distribution Inc.). The plant materials were kept in the greenhouse at 20°C/ 25°C (night/day),15-hrs of light, and watered twice a week for 4-weeks. Urediniospores were recovered from -80°C and allowed to acclimate at room temperature for 1 h. For each rust, 20 ml of suspension containing 1×105 urediniospores ml−1 and 5 μl of Tween-twenty (Agdia Inc. Elkhart, IN) were used to inoculate 6 pots; while 6 control pots were sprayed with sterile water. After inoculation, plants were allowed to dry for 1 h and then transferred to a dark chamber at 20°C and 90% of humidity for 12-15 h. At 10-days post inoculation, all inoculated plants developed rust symptoms identical to those observed in the field, whereas control plants had no symptoms. Stem and crown rust pathogens were re-isolated from the artificially inoculated tall fescue plants. Based on form, size, color and numbers of cells forming the spores, a 1947 Festuca elatior specimen from Georgia mentioning Puccinia coronata (Hanlin 1966), held at the Julian H. Miller Mycological Herbarium (Catalog No. GAM00013162), was discarded as an earlier record of P. coronata var. avenae and could have been misdiagnosed. Due to the fragile integrity of the original infected plant sample as well as the incipient infection, DNA identification was unsuccessful. To our knowledge, this is the first morphological, genetic and taxonomic report of P. graminis and P. coronata var. avenae f. sp. avenae on tall fescue in Georgia, USA

show abstract

Incidence and Distribution of Puccinia coronata and P. graminis on Turfgrass in the Midwestern United States

Cited by 3 publications

References 33 publications

Türkiye'deki çim alanlarında Puccinia spp.nin qPCR analizi

Türkiye'deki çim alanlarında Puccinia spp.nin qPCR analizi

The U.S. Capitol Grounds and Arboretum: an iconic landscape with a holistic approach to pest management

First Report of Stem Rust, Caused by Puccinia graminis, and Crown Rust, Caused by Puccinia coronata var. avenae f. sp. avenae, on Tall Fescue (Festuca arundinacea) in Georgia, U.S.A.

Contact Info

Product

Resources

About