Incidence and characterization of Potato virus V infections in Iran

Abstract

A survey was carried out to determine the extent of Potato virus V (PVV) infection, together with other potyviruses, in Iran in both commercial and local potato varieties. We found a low incidence of PVV in commercial varieties compared to a local potato cultivar Zardi, in which we noted a PVV infection up to *32.9 %. We determined the genomic sequence 9,812 nucleotides of one isolate (KER.LAL.P) from cultivar Agria and the 3 0 -terminal sequence including coat protein (CP) gene of four additional isolates from cultivar Zardi. The Iranian isolate PVV KER.LAL.P was found to share 91 % sequence identity with the Scottish isolate DV-42 (AJ243766) of PVV. The CP gene sequences of the PVV isolates from Iran shared 96.5 to 99.3 % pairwise nucleotide identity and they shared \97.5 % pairwise identity with the CP sequences of all other PVV isolates available in public databases. Our host studies indicated that the Iranian PVV isolate had a narrower host range and infected Nicotiana debneyi and N. glutinosa test plants. Within the commercial varieties of potato in Iran, we noted a significant amount of mixed potyvirus infection. This study is the first report of occurrence and complete genome of PVV in Iran.

Caracterización molecular del Potato virus V (PVV) infectando Solanum phureja mediante secuenciación de nueva generación

“…Zardi) (Shamsadden-Saeed et al, 2014) y Costa Rica (37 % en S. tuberosum) (Vásquez et al, 2006), y a que ha sido declarado como virus cuarentenario para China, el principal productor de papa del mundo con cerca del 26,3% del área global destinada a este cultivo (Wang et al, 2011), son muy pocos los estudios en los que se han evaluado sus características genómicas y sus niveles de variación intraespecíficos. Esta situación se ve claramente reflejada por el reporte de tan sólo tres genomas completos de PVV en el mundo: DV42 de Escocia (Oruetxebarria et al, 2000), KER.LAL.P de Irán (Shamsadden-Saeed et al, 2014), y PVV-Phureja de Colombia . En este último estudio, se secuenció el transcriptoma de tejido foliar de una sola planta de S. phureja var.…”

“…El hallazgo de esta última especie (PVV) sobre S. phureja en Antioquia resultó inesperado por cuanto los síntomas de mosaicos que presentaban las plantas infectadas habían sido asociados con la infección por PVY. PVV ha sido reportado en Perú (Spetz et al, 2003), Costa Rica (Vásquez et al, 2006), Estados Unidos (Shiel et al, 2004), Europa continental e Inglaterra (Oruetxebarria et al, 2000;Mortensen et al, 2010) y en Irán (Shamsadden-Saeed et al, 2014).…”

“…El virus consiste de partículas flexuosas de 760 nm, cuyo material genético es ARN de cadena sencilla positiva (ssRNA+) con cerca de 9900 nt (excluyendo la cola de poli-A). Su genoma contiene un gran marco de lectura abierto (ORF) que codifica para una poliproteína de 3066-3068 residuos y presenta regiones no traducidas (UTR) de aproximadamente 200 (3´UTR) y 445 nt (5´UTR), respectivamente (Oruetxebarria et al, 2000;Shamsadden-Saeed et al, 2014;Gutiérrez et al, 2016).…”

Genome sequence of a divergent Colombian isolate of potato virus V (PVV) infecting Solanum phureja

“…PVV can be transmitted by infected seed tubers and non-persistently by aphids such as Myzus persicae, Brachycaudus helichrysi, Macrosiphum euphorbiae and Rhopalosiphoninus latysiphon (Calvert et al, 1980;Bell, 1983;Fribourg and Nakashima, 1984). Potato virus V has been reported to infect potato cultivars in the Andean region of South America (Spetz et al, 2003), Northwestern Europe (Oruetxebarria et al, 2000) and, more recently, Iran (Shamsadden-Saeed et al, 2014). Th is limited distribution is apparently due to the presence of hypersensitive resistance genes and broad-spectrum resistance in potato cultivars (Barker 1997;Valkonen 2008).…”

“…To date, only two PVV genomes have been fully sequenced: DV42 from Scotland (Oruetxebarria et al, 2000) and KER. LAL.P from Iran (Shamsadden-Saeed et al, 2014) and, in spite of being considered the center of origin of PVV (Spetz et al, 2003), there are no complete genomes of PVV isolates from the South American Andes. To better characterize the PVV variant infecting S. phureja in Colombia, we have sequenced the complete genome of an isolate derived from an infected plant showing mild mosaics and discuss its phylogenetic relation to other PVV isolates.…”