IncA/C Plasmid Carrying
 bla
 NDM-1
 ,
 bla
 CMY-16
 , and
 fosA3
 in a Salmonella enterica Serovar Corvallis Strain Isolated from a Migratory Wild Bird in Germany

Villa, Laura; Guerra, Beatriz; Schmoger, Silvia; Fischer, Jennie; Helmuth, Reiner; Zong, Zhiyong; García-Fernández, Aurora; Carattoli, Alessandra

doi:10.1128/aac.00944-15

Cited by 70 publications

(63 citation statements)

References 17 publications

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“…On both of our IncB/O plasmids and C629 plasmid unnamed1 there was a remnant (131 bp) of ISAba125 upstream of bla NDM , confirming the hypothesis that ISAba125 is closely linked with the original mobilization of bla NDM (17). Although genes fosA3 and bla NDM-1 on the same plasmid were found in Salmonella from a migratory wild bird in Germany (18), fosA3 was located ϳ73 kb away from bla NDM-1 in that plasmid. The annotation and blast results in our study revealed a novel gene arrangement and that the new fosA3-bla NDM-9 resistance region might be developed by acquiring a resistant gene through stepwise integration and/or recombination events mediated by IS26 and ISAba125.…”

Section: Table 1 Characterization Of Mcr-1-positive Isolates and Theisupporting

confidence: 78%

Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

Liu

Song

Zou

et al. 2017

Antimicrob Agents Chemother

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We report the presence of mcr-1 in Escherichia coli and carbapenemresistant Cronobacter sakazakii from the same diseased chicken. The mcr-1 gene linked with ISApl1 was located on two different IncI2 plasmids, including one multidrug plasmid in E. coli, whereas fosA3-bla NDM-9 was on an IncB/O plasmid in C. sakazakii. The development of the fosA3-bla NDM-9 resistance region was mediated by IS26. The colocation of mcr-1 or bla NDM-9 with other resistance genes will accelerate the dissemination of the two genes.KEYWORDS mcr-1, NDM-9, Cronobacter sakazakii, chicken, insertion sequences S ince the plasmid-mediated colistin resistance gene mcr-1 was first reported in Escherichia coli isolates in China (1), several reports confirmed that mcr-1 has spread in several Enterobacteriaceae species on different continents (2-5). Of great clinical concern are the inevitable concurrence of mcr-1 and carbapenem-resistance genes among Enterobacteriaceae and the massive use of colistin in animals, which could aggravate the selection process, resulting in the emergence of true pan-drug resistance. To date, the emergence of mcr-1 has been reported in carbapenem-resistant E. coli and Klebsiella pneumoniae (3, 6, 7). Here, we report the presence of mcr-1 in E. coli and the carbapenem-resistant Cronobacter sakazakii strain recovered from the same diseased chicken in China. We also report a new gene arrangement of the fosA3-bla NDM-9 resistance region.During a surveillance study in 2015 for E. coli susceptibility to carbapenem on animal farms in Shandong province, China, two carbapenem-resistant Cronobacter sakazakii isolates, WF5-19C and WF5-21C, were collected from two different diseased chickens with diarrhea at a chicken farm. Further information about these animals, the underlying disease, and possible antimicrobial pretreatment in the farm was unfortunately not available. Antimicrobial susceptibilities were assayed according to the guidelines provided by the Clinical and Laboratory Standards Institute (CLSI) (8). The breakpoints for each antimicrobial were recommended by the CLSI and veterinary CLSI (8, 9). We found that the two isolates showed resistances to each of the antimicrobial drugs tested, including colistin (MICs, 4 g/ml) (European Committee on Antimicrobial Susceptibility Testing clinical breakpoint for colistin resistance, Ն2 g/ml) (Table 1). Interestingly, E. coli isolate WF5-19 from the same chicken with C. sakazakii WF5-19C was also resistant to colistin and other antimicrobial drugs (except meropenem and imipenem). We genotyped the three carbapenem-and/or colistin-resistant isolates for the presence of carbapenemase genes and mcr-1 using PCR as described previously (1, 10, 11). The mcr-1 gene was detected in all isolates. In addition, sequencing of the bla NDM amplicons confirmed that both C. sakazakii isolates produced the NDM-9

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Section: Table 1 Characterization Of Mcr-1-positive Isolates and Theisupporting

confidence: 78%

Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

Liu

Song

Zou

et al. 2017

Antimicrob Agents Chemother

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“…ST1 is strongly associated with the carriage of bla NDM (15/20 plasmids; 75%), while only two plasmids outside ST1 carry bla NDM (one each from ST3 and ST6). Comparison of the bla NDM genetic location has shown multiple genetic organizations (41)(42)(43)(44)(45)(46). Within our database, we observed a total of seven distinct bla NDM structures (see Fig.…”

Section: Figmentioning

confidence: 72%

Identification of IncA/C Plasmid Replication and Maintenance Genes and Development of a Plasmid Multilocus Sequence Typing Scheme

Hancock

Phan

Peters

et al. 2017

Antimicrob Agents Chemother

108

112

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Plasmids of incompatibility group A/C (IncA/C) are becoming increasingly prevalent within pathogenic Enterobacteriaceae. They are associated with the dissemination of multiple clinically relevant resistance genes, including bla CMY and bla NDM . Current typing methods for IncA/C plasmids offer limited resolution. In this study, we present the complete sequence of a bla NDM-1 -positive IncA/C plasmid, pMS6198A, isolated from a multidrug-resistant uropathogenic Escherichia coli strain. Hypersaturated transposon mutagenesis, coupled with transposon-directed insertion site sequencing (TraDIS), was employed to identify conserved genetic elements required for replication and maintenance of pMS6198A. Our analysis of TraDIS data identified roles for the replicon, including repA, a toxin-antitoxin system; two putative partitioning genes, parAB; and a putative gene, 053. Construction of mini-IncA/C plasmids and examination of their stability within E. coli confirmed that the region encompassing 053 contributes to the stable maintenance of IncA/C plasmids. Subsequently, the four major maintenance genes (repA, parAB, and 053) were used to construct a new plasmid multilocus sequence typing (PMLST) scheme for IncA/C plasmids. Application of this scheme to a database of 82 IncA/C plasmids identified 11 unique sequence types (STs), with two dominant STs. The majority of bla NDMpositive plasmids examined (15/17; 88%) fall into ST1, suggesting acquisition and subsequent expansion of this bla NDM -containing plasmid lineage. The IncA/C PMLST scheme represents a standardized tool to identify, track, and analyze the dissemination of important IncA/C plasmid lineages, particularly in the context of epidemiological studies.

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“…New subtypes, with similar structure, of the gene have been described (fosA2, fosA3, fosA4, and fosA5) (108)(109)(110). Cooccurrence in plasmids with bla CTX-M , bla NDM , bla KPC , bla OXA , bla CMY , bla AmpC , bla TEM , bla SHV , bla SFO-1 , gyrA, parC, parE, sul1, sul2, strA, strB, aac(6=)-Ib, aadA5, aphA6, tetA(A), mphA, floR, dfrA7, rmtB, and merA genes has been reported, conferring resistance to ␤-lactams, quinolones, aminoglycosides, macrolides, sulfonamides, and tetracyclines (75,80,(111)(112)(113)(114). The genes conferring resistance to fosfomycin could be transferred together with genes conferring resistance to other antibiotics in either the same or a conjugate plasmid (114).…”

Section: Acquired Resistancementioning

confidence: 99%

“…Finally, the mutations described above can be found in chromosomes, but the emergence of resistant loci in plasmids (FosA and FosB) could potentially provide a better means for the spread of resistance mechanisms than the chromosomal ones (126). Several recently published studies have shown dissemination of fosfomycin-resistant strains (mainly due to the presence of FosA) in patients as well environmental reservoirs in livestock and animals (75,113,127,128).…”

Section: In Vitro and In Vivo Development Of Resistance And Spreadmentioning

confidence: 99%

Fosfomycin

et al. 2016

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SUMMARYThe treatment of bacterial infections suffers from two major problems: spread of multidrug-resistant (MDR) or extensively drug-resistant (XDR) pathogens and lack of development of new antibiotics active against such MDR and XDR bacteria. As a result, physicians have turned to older antibiotics, such as polymyxins, tetracyclines, and aminoglycosides. Lately, due to development of resistance to these agents, fosfomycin has gained attention, as it has remained active against both Gram-positive and Gram-negative MDR and XDR bacteria. New data of higher quality have become available, and several issues were clarified further. In this review, we summarize the available fosfomycin data regarding pharmacokinetic and pharmacodynamic properties, thein vitroactivity against susceptible and antibiotic-resistant bacteria, mechanisms of resistance and development of resistance during treatment, synergy and antagonism with other antibiotics, clinical effectiveness, and adverse events. Issues that need to be studied further are also discussed.

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IncA/C Plasmid Carrying bla _NDM-1 , bla _CMY-16 , and fosA3 in a Salmonella enterica Serovar Corvallis Strain Isolated from a Migratory Wild Bird in Germany

Cited by 70 publications

References 17 publications

Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

Identification of IncA/C Plasmid Replication and Maintenance Genes and Development of a Plasmid Multilocus Sequence Typing Scheme

Fosfomycin

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IncA/C Plasmid Carrying bla NDM-1 , bla CMY-16 , and fosA3 in a Salmonella enterica Serovar Corvallis Strain Isolated from a Migratory Wild Bird in Germany

Cited by 70 publications

References 17 publications

Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal

Identification of IncA/C Plasmid Replication and Maintenance Genes and Development of a Plasmid Multilocus Sequence Typing Scheme

Fosfomycin

Contact Info

Product

Resources

About

IncA/C Plasmid Carrying bla _NDM-1 , bla _CMY-16 , and fosA3 in a Salmonella enterica Serovar Corvallis Strain Isolated from a Migratory Wild Bird in Germany