Inbreeding by Environmental Interactions Affect Gene Expression in <i>Drosophila melanogaster</i>

Kristensen, Torsten Nygaard; Sørensen, Peter; Pedersen, Kamilla; Kruhøffer, Mogens; Loeschcke, Volker

doi:10.1534/genetics.105.054486

Cited by 77 publications

(118 citation statements)

References 33 publications

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“…The relationship between stress and within-line, or microenvironmental, variance is not as clear, in part because studies tend to report within-line variance but do not test hypotheses about it (for example, Fry and Heinsohn, 2002). Several studies have documented increases in microenvironmental variance with exposure to a new, and generally stressful, environment (for example, Rutter et al, 2010); this increased variance could reflect variance in expression of individual genes (for example, Kristensen et al, 2006). As expected, given previous work with Caenorhabditis MA lines (Baer, 2008;Matsuba et al, 2012), we detected higher within-line variance in the low-fitness MA lines than in the high-fitness MA lines.…”

Section: Discussionsupporting

confidence: 81%

Abiotic stress does not magnify the deleterious effects of spontaneous mutations

et al. 2015

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Although the effects of deleterious alleles often are predicted to be greater in stressful environments, there is no theoretical basis for this prediction and the empirical evidence is mixed. Here we characterized the effects of three types of abiotic stress (thermal, oxidative and hyperosmotic) on two sets of nematode (Caenorhabditis elegans) mutation accumulation (MA) lines that differ by threefold in fitness. We compared the survival and egg-to-adult viability between environments (benign and stressful) and between fitness categories (high-fitness MA, low-fitness MA). If the environment and mutation load have synergistic effects on trait means, then the difference between the high and low-fitness MA lines should be larger in stressful environments. Although the stress treatments consistently decreased survival and/or viability, we did not detect significant interactions between fitness categories and environment types. In contrast, we did find consistent evidence for synergistic effects on (micro) environmental variation. The lack of signal in trait means likely reflects the very low starting fitness of some low-fitness MA lines, the potential for cross-stress responses and the context dependence of mutational effects. In addition, the large increases in the environmental variance in the stressful environments may have masked small changes in trait means. These results do not provide evidence for synergism between mutation and stress.

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Section: Discussionsupporting

confidence: 81%

Abiotic stress does not magnify the deleterious effects of spontaneous mutations

et al. 2015

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“…Recent studies showed that several pathways may be upregulated in inbred as compared with outbred Drosophila individuals, whereas others, though less commonly occurring, are downregulated (Kristensen et al, , 2006Pedersen et al, 2005;García et al, 2012). Similarly, Ayroles et al (2009) showed that inbreeding depression results in an overexpression of many genes associated with metabolism, stress or defence mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Inbreeding interferes with the heat-shock response

Franke

Fischer

2014

Heredity

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Inbreeding is typically detrimental to individual fitness, with negative effects being often exaggerated in stressful environments. However, the causal mechanisms underlying inbreeding depression in general and the often increased susceptibility to stress in particular are not well understood. We here test whether inbreeding interferes with the heat-shock response, comprising an important component of the stress response which may therefore underscore sensitivity to stress. To this end we subjected the tropical butterfly Bicyclus anynana to a full-factorial design with three temperatures and three levels of inbreeding, and measured the expression of heat-shock protein (HSP) 70 via qPCR. HSP70 expression increased after exposure to heat as compared with cold or control conditions. Most strikingly, inbreeding strongly interfered with the heat-shock response, with inbred individuals showing a very weak upregulation of HSP70 only. Our results thus indicate that, in our study organism, interference with the heat-shock response may be one mechanism underlying reduced fitness of inbred individuals, especially when exposed to stressful conditions. However, these indications need to be corroborated using a broader range of different temperatures, genes and taxa.

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“…In essence, this suggests that dysfunction of mitochondrial function is a major factor in the high mortality observed in the L-line. Other analyses on the molecular consequences of environmental stress, inbreeding and aging have also observed genes related to energy metabolism to be affected (Landis et al, 2004;Kristensen et al, 2006;S rensen et al, 2007). This indicates that energy metabolism is a highly sensitive and fine-tuned process that can respond to a diverse range of stimuli.…”

Section: Mitochondrial-related Processesmentioning

confidence: 93%

Proteomic characterization of a temperature-sensitive conditional lethal in Drosophila melanogaster

et al. 2009

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Genetic variation that is expressed only under specific environmental conditions can contribute to additional adverse effects of inbreeding if environmental conditions change. We present a proteomic characterization of a conditional lethal found in an inbred line of Drosophila melanogaster. The lethal effect is apparent as a large increase in early mortality at the restrictive temperature (29 1C) as opposed to normal survival at the permissive temperature (20 1C). The increased mortality in response to the restrictive temperature is probably caused by a single recessive major locus. A quantitative trait locus (QTL) region segregating variation affecting the lethal effect has been identified, allowing for a separation of primary/causal effects and secondary consequences in the proteome expression patterns observed. In this study, the proteomic response to the restrictive temperature in the lethal-line (L-line) was compared with the response in an inbred-control-line (IC-line) and an outbred-control-line (OC-line). Quantitative protein changes were detected using isobaric tags for relative and absolute quantitation (iTRAQ) two-dimensional liquid chromatography-tandem mass spectrometry. In all, 45 proteins were found to be significantly differently regulated in response to the restrictive temperature in the L-line as compared with the IC-line. No proteins were significantly differently regulated between the IC-line and the OC-line, verifying that differential protein regulation was specific to a genetic defect in the L-line. Proteins associated with oxidative phosphorylation and mitochondria were significantly overrepresented within the list of differentially expressed proteins. Proteins related to muscle contraction were also found to be differentially expressed in the L-line in response to the restrictive temperature, supporting phenotypic observations of moribund muscle hypercontraction.

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Inbreeding by Environmental Interactions Affect Gene Expression in Drosophila melanogaster

Cited by 77 publications

References 33 publications

Abiotic stress does not magnify the deleterious effects of spontaneous mutations

Abiotic stress does not magnify the deleterious effects of spontaneous mutations

Inbreeding interferes with the heat-shock response

Proteomic characterization of a temperature-sensitive conditional lethal in Drosophila melanogaster

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