Inappropriate annotation of a key defence marker in Arabidopsis: will the real PR-1 please stand up?

Laird, Janet; Armengaud, Patrick; Giuntini, Pietro; Laval, Valérie; Milner, Joel J.

doi:10.1007/s00425-004-1355-x

Cited by 39 publications

(31 citation statements)

References 13 publications

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“…Furthermore, VSP2, whose transcript level is strongly decreased in response to exogenous ET (Rojo et al, 1999;Van Zhong and Burns, 2003), was up-regulated during K 1 starvation. Similarly, searching our data for known SAresponsive genes such as PR1-like (At2g14610; Uknes et al, 1992;Laird et al, 2004), PR5 (At1g75040), NPR1 (At1g64280; Cao et al, 1997), and WRKY18 (At4g31800; Yu et al, 2001) revealed no indication for increased SA production during K 1 starvation. Cross-talk between SA, JA, and ET has been modeled from gene expression data obtained from Pseudomonas syringae infected signaling-defective Arabidopsis mutants (using the Affymetrix 8K microarray; Glazebrook et al, 2003).…”

Section: Discussionmentioning

confidence: 87%

The Potassium-Dependent Transcriptome of Arabidopsis Reveals a Prominent Role of Jasmonic Acid in Nutrient Signaling

2004

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Full genome microarrays were used to assess transcriptional responses of Arabidopsis seedlings to changing external supply of the essential macronutrient potassium (K+). Rank product statistics and iterative group analysis were employed to identify differentially regulated genes and statistically significant coregulated sets of functionally related genes. The most prominent response was found for genes linked to the phytohormone jasmonic acid (JA). Transcript levels for the JA biosynthetic enzymes lipoxygenase, allene oxide synthase, and allene oxide cyclase were strongly increased during K+ starvation and quickly decreased after K+ resupply. A large number of well-known JA responsive genes showed the same expression profile, including genes involved in storage of amino acids (VSP), glucosinolate production (CYP79), polyamine biosynthesis (ADC2), and defense (PDF1.2). Our findings highlight a novel role of JA in nutrient signaling and stress management through a variety of physiological processes such as nutrient storage, recycling, and reallocation. Other highly significant K+-responsive genes discovered in our study encoded cell wall proteins (e.g. extensins and arabinogalactans) and ion transporters (e.g. the high-affinity K+ transporter HAK5 and the nitrate transporter NRT2.1) as well as proteins with a putative role in Ca2+ signaling (e.g. calmodulins). On the basis of our results, we propose candidate genes involved in K+ perception and signaling as well as a network of molecular processes underlying plant adaptation to K+ deficiency.

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Section: Discussionmentioning

confidence: 87%

The Potassium-Dependent Transcriptome of Arabidopsis Reveals a Prominent Role of Jasmonic Acid in Nutrient Signaling

2004

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“…In the tumor, several genes involved in ET perception and signaling were activated. By contrast, genes involved in JA signaling, such as JAZ family members, COI1 (At2g39940; Katsir et al, 2008;Staswick, 2008), or MYC2 (At1g32640; Lorenzo et al, 2004) as well as the two classical marker genes of the SA-induced SAR response pathway, PR1 (At2g14610; Laird et al, 2004) and NPR1 (At1g64280), were never found to be activated. This observation suggests that the auxin and ET signaling pathways, rather than SA-induced SAR, seems to be induced in the host during Arabidopsis-Agrobacterium interaction.…”

Section: H 2 O 2 Accumulation Is Prevented At the Beginning Of The Inmentioning

confidence: 94%

Agrobacterium tumefaciensPromotes Tumor Induction by Modulating Pathogen Defense inArabidopsis thaliana

et al. 2009

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Agrobacterium tumefaciens causes crown gall disease by transferring and integrating bacterial DNA (T-DNA) into the plant genome. To examine the physiological changes and adaptations during Agrobacterium-induced tumor development, we compared the profiles of salicylic acid (SA), ethylene (ET), jasmonic acid (JA), and auxin (indole-3-acetic acid [IAA]) with changes in the Arabidopsis thaliana transcriptome. Our data indicate that host responses were much stronger toward the oncogenic strain C58 than to the disarmed strain GV3101 and that auxin acts as a key modulator of the ArabidopsisAgrobacterium interaction. At initiation of infection, elevated levels of IAA and ET were associated with the induction of host genes involved in IAA, but not ET signaling. After T-DNA integration, SA as well as IAA and ET accumulated, but JA did not. This did not correlate with SA-controlled pathogenesis-related gene expression in the host, although high SA levels in mutant plants prevented tumor development, while low levels promoted it. Our data are consistent with a scenario in which ET and later on SA control virulence of agrobacteria, whereas ET and auxin stimulate neovascularization during tumor formation. We suggest that crosstalk among IAA, ET, and SA balances pathogen defense launched by the host and tumor growth initiated by agrobacteria.

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“…Among the biochemical pathway and physiological function marker loci represented on our microarrays, transcripts coding for 4-coumarate-CoA ligase (4-CL), chalcone isomerase (CHI), NADPH-dependent P450 reductase (ATR3), glyceraldehyde 3-P dehydrogenase A (GapA), copper/zinc superoxidase dismutase (CSD1), vegetative storage protein (Vsp1), Asn synthetase (ASN1), and a number of other loci were expressed higher in the Col ecotype. In contrast, only transcripts coding for cytochrome b 5 , a pathogenesisrelated 1 (PR1)-similar protein, and two other unassigned proteins were expressed higher in the Ler ecotype; the PR1-similar transcript is related to the plant defense protein expressed in response to pathogen infection and treatment with resistance-inducing compounds (Laird et al, 2004).…”

Section: Transcript Profiling In Col and Ler Ecotypesmentioning

confidence: 98%

Variations in CYP74B2 (Hydroperoxide Lyase) Gene Expression Differentially Affect Hexenal Signaling in the Columbia and Landsberg erecta Ecotypes of Arabidopsis

et al. 2005

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The CYP74B2 gene in Arabidopsis (Arabidopsis thaliana) ecotype Columbia (Col) contains a 10-nucleotide deletion in its first exon that causes it to code for a truncated protein not containing the P450 signature typical of other CYP74B subfamily members. Compared to CYP74B2 transcripts in the Landsberg erecta (Ler) ecotype that code for full-length hydroperoxide lyase (HPL) protein, CYP74B2 transcripts in the Col ecotype accumulate at substantially reduced levels. Consistent with the nonfunctional HPL open reading frame in the Col ecotype, in vitro HPL activity analyses using either linoleic acid hydroperoxide or linolenic acid hydroperoxide as substrates show undetectable HPL activity in the Col ecotype and C6 volatile analyses using leaf homogenates show substantially reduced amounts of hexanal and no detectable trans-2-hexenal generated in the Col ecotype. P450-specific microarrays and full-genome oligoarrays have been used to identify the range of other transcripts expressed at different levels in these two ecotypes potentially as a result of these variations in HPL activity. Among the transcripts expressed at significantly lower levels in Col leaves are those coding for enzymes involved in the synthesis of C6 volatiles (LOX2, LOX3), jasmonates (OPR3, AOC), and aliphatic glucosinolates (CYP83A1, CYP79F1, AOP3). Two of the three transcripts coding for aliphatic glucosinolates (CYP83A1, AOP3) are also expressed at significantly lower levels in Col flowers.

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Inappropriate annotation of a key defence marker in Arabidopsis: will the real PR-1 please stand up?

Cited by 39 publications

References 13 publications

The Potassium-Dependent Transcriptome of Arabidopsis Reveals a Prominent Role of Jasmonic Acid in Nutrient Signaling

The Potassium-Dependent Transcriptome of Arabidopsis Reveals a Prominent Role of Jasmonic Acid in Nutrient Signaling

Agrobacterium tumefaciensPromotes Tumor Induction by Modulating Pathogen Defense inArabidopsis thaliana

Variations in CYP74B2 (Hydroperoxide Lyase) Gene Expression Differentially Affect Hexenal Signaling in the Columbia and Landsberg erecta Ecotypes of Arabidopsis

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