Inactive Renin in Human Plasma

Derkx, F. H. M.; Wenting, G. J.; Veld, A. J. Man In 'T; Gool, Jeanette M.G. van; Verhoeven, R. P.; Schalekamp, M. A. D. H.

doi:10.1016/s0140-6736(76)90791-1

Cited by 119 publications

(69 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although many investigators have reported changes in the levels of prorenin in response to a variety of physiologic and pharmacologic maneuvers in both normal subjects and patients with disorders affecting the renin-angiotensin system (4,8,9), a unified view of the relationship of the prozymogen to active renin has not yet emerged. To date, studies of the physiologic regulation of renin biosynthesis and processing in vivo have employed in vitro activation techniques and enzymatic assays in which plasma renin levels are quantitated by measurement of the rate at which angiotensin is released from renin substrate (angiotensinogen).…”

Section: Introductionmentioning

confidence: 99%

Response of plasma prorenin and active renin to chronic and acute alterations of renin secretion in normal humans. Studies using a direct immunoradiometric assay.

Toffelmire¹,

Slater²,

Corvol³

et al. 1989

J. Clin. Invest.

View full text Add to dashboard Cite

We employed a novel immunoradiometric assay to measure plasma levels of active renin and prorenin in physiologic and pharmacologic studies designed to characterize renin biosynthesis and processing in response to both chronic and acute stimuli of renin secretion in normal human subjects. Stimulation of renin secretion with prolonged dietary sodium restriction or amiloride resulted in marked increases in the plasma levels of prorenin, active renin, and plasma renin activity (PRA); suppression of renin secretion with indomethacin resulted in parallel decreases in prorenin, active renin, and PRA. In contrast, acute stimulation with upright activity or administration of an angiotensin-converting enzyme inhibitor, which increased active renin and PRA from 2-to 15-fold, had no effect on prorenin levels. Based on studies in cultured human juxtaglomerular tumor cells, it has been proposed that prorenin is secreted constitutively whereas active renin is stored in and released from secretory granules through a regulated pathway. Our studies are consistent with such a model: the parallel changes in active renin and prorenin with experimental maneuvers of long duration suggest that both the constitutive and regulated pathways are altered under these conditions. The increase in active renin levels in the absence of a change in prorenin that occurs in response to acute stimuli presumably represents the release of preformed active enzyme that is stored in secretory granules.

show abstract

Section: Introductionmentioning

confidence: 99%

Response of plasma prorenin and active renin to chronic and acute alterations of renin secretion in normal humans. Studies using a direct immunoradiometric assay.

Toffelmire¹,

Slater²,

Corvol³

et al. 1989

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…The measurements of plasma inactive renin reported by many authors are widely scattered (Derkx et al 1976(Derkx et al , 1978Leckie et al 1977;Sealey et al 1977 a,b ;Kappelgaard et al 1978;Millar et al 1980). Where does this inconsistency come from?…”

Section: Discussionmentioning

confidence: 98%

“…Inactive renin may be released from the kidney directly (Derkx et al 1976;Birkenhager et al 1978;Millar et al 1978;Derkx et al 1983), or active renin may be converted to inactive form in the circulation (Vandongen et al 1977;Aoi et al 1979Aoi et al , 1981. But in any case, it seems reasonable to assume that TRC, i.e.…”

Section: Discussionmentioning

confidence: 99%

Active, inactive and total renin concentrations in plasma of hypertensive patients.

Goto

Abe

Tsunoda

et al. 1985

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

“…Appropriate assays were hence developed for the separate assessment of prorenin. [34][35][36][37][38] The relative proportions of active renin and prorenin vary very considerably in different circumstances and diseases ( Figure 3). …”

Section: Circulating Inactive Renin (Prorenin)mentioning

confidence: 99%