Inactivation of Yeast Glutathione Reductase by Fenton Systems: Effect of Metal Chelators, Catecholamines and Thiol Compounds

Gutiérrez-Correa, José; Stoppani, A. O. M.

doi:10.3109/10715769709097858

Cited by 41 publications

(23 citation statements)

References 42 publications

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“…17 It is possible that albumin acts as a "sacrificial" antioxidant to reduce injury during reperfusion. 18 Isoprostanes represent products of lipid peroxidation and are indicative of superoxide production. 19 In our study, iP increased in response to coronary artery occlusion during PCI in 9 of 19 patients similar to data from previous studies, 14 -16 but was not a reliable marker of ischemia.…”

mentioning

confidence: 99%

Ischemia Modified Albumin Is a Sensitive Marker of Myocardial Ischemia After Percutaneous Coronary Intervention

Sinha

Gaze²,

Tippins³

et al. 2003

Circulation

195

122

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Background-Ischemia modified albumin (IMA; Ischemia Technologies, Inc) blood levels rise in patients who develop ischemia during percutaneous coronary intervention (PCI). It is not known whether IMA elevations correlate with increases in other markers of oxidative stress, ie, 8-iso prostaglandin F2-A (iP). Methods and Results-We compared IMA versus iP plasma levels in 19 patients (mean age 62.8Ϯ11.9 years) undergoing PCI and 11 patients (mean age 64Ϯ13.6 years) undergoing diagnostic angiography (controls). In the PCI patients, blood samples for IMA and iP were taken from the guide catheter before PCI and after balloon inflations, and from the femoral sheath 30 minutes after PCI. IMA was measured by the albumin cobalt binding (ACB) test and plasma iP by enzyme immunoassay. During PCI, all 19 patients had chest pain and 18 had transient ischemic ST segment changes. IMA was elevated from baseline in 18 of the 19 patients after PCI. Median IMA levels were higher after PCI (101.4 U/mL, 95%CI 82 to 116) compared with baseline (72.8 U/mL, CI 55 to 93; PϽ0.0001). Levels remained elevated at 30 minutes (87.9 U/mL, CI 78 to 99; PϽ0.0001) and returned to baseline at 12 hours (70.3 U/mL, CI 65 to 87; Pϭ0.65). iP levels were raised after PCI in 9 of the 19 patients. However, median iP levels were not significantly different immediately (Pϭ0.6) or 30 minutes after PCI (Pϭ0.1). In the control group, IMA and iP levels remained unchanged before and after angiography (Pϭ0.2 and 0.16, respectively). Conclusions-IMA is a more consistent marker of ischemia than iP in patients who develop chest pain and ST segment changes during PCI. Key Words: ischemia Ⅲ proteins Ⅲ angioplasty I schemia modified albumin (IMA; Ischemia Technologies, Inc), measured by the albumin cobalt binding test (ACB; Ischemia Technologies), has been shown to be a marker of myocardial ischemia. 1 ACB measures the ability of the amino terminus (N-terminus) of human albumin to bind exogenous cobalt. Under physiological conditions, transition metals can bind tightly to the exposed N-terminus of albumin. 2 In the presence of myocardial ischemia, structural changes take place in the N-terminus of the protein, 3,4 which reduce its binding capacity, possibly, in part, as a result of exposure to reactive oxygen species (ROS). IMA rises in patients who develop myocardial ischemia during percutaneous coronary intervention (PCI). 5 PCI offers a clinical model of myocardial ischemia-reperfusion characterized by an increase in oxidative stress. 6 -8 ROS are produced after myocardial ischemia-reperfusion and result in the production of lipid and protein peroxidation products in the local myocardial environment. This occurs irrespective of the mechanism responsible for ischemia, ie, increased oxygen demand or a primary reduction of coronary blood flow. 9 8-epi prostaglandin F 2␣ (iP), a prostaglandin isomer, is formed by free-radical catalyzed oxidation of arachidonic acid esterified in membrane phospholipids. 10 It represents a chemically stable end-product of lipid peroxidat...

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mentioning

confidence: 99%

Ischemia Modified Albumin Is a Sensitive Marker of Myocardial Ischemia After Percutaneous Coronary Intervention

Sinha

Gaze²,

Tippins³

et al. 2003

Circulation

195

122

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“…GR also contains an active site Cys residue [50]. GR is less-readily inhibited than GAPDH by H 2 O 2 , and loss of activity has been reported to require metal ions, be radicalmediated, involve oxidation of other residues in addition to the active site Cys (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…GR is less-readily inhibited than GAPDH by H 2 O 2 , and loss of activity has been reported to require metal ions, be radicalmediated, involve oxidation of other residues in addition to the active site Cys (e.g. His467, Tyr114 and Trp residues [50]), and result in the formation of carbonyl groups [50][51][52]. A preliminary report has appeared on the inhibition of GR by radiation-generated peroxides [34].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack

Morgan¹,

Dean²,

Davies³

2002

Eur J Biochem

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Reaction of certain peptides and proteins with singlet oxygen (generated by visible light in the presence of rose bengal dye) yields long-lived peptide and protein peroxides. Incubation of these peroxides with glyceraldehyde-3-phosphate dehydrogenase, in the absence of added metal ions, results in loss of enzymatic activity. Comparative studies with a range of peroxides have shown that this inhibition is concentration, peroxide, and time dependent, with H 2 O 2 less efficient than some peptide peroxides. Enzyme inhibition correlates with loss of both the peroxide and enzyme thiol residues, with a stoichiometry of two thiols lost per peroxide consumed. Blocking the thiol residues prevents reaction with the peroxide. This stoichiometry, the lack of metal-ion dependence, and the absence of electron paramagnetic resonance (EPR)-detectable species, is consistent with a molecular (nonradical) reaction between the active-site thiol of the enzyme and the peroxide. A number of low-molecular-mass compounds including thiols and ascorbate, but not Trolox C, can prevent inhibition by removing the initial peroxide, or species derived from it. In contrast, glutathione reductase and lactate dehydrogenase are poorly inhibited by these peroxides in the absence of added Fe 2+ -EDTA. The presence of this metal-ion complex enhanced the inhibition observed with these enzymes consistent with the occurrence of radicalmediated reactions. Overall, these studies demonstrate that singlet oxygen-mediated damage to an initial target protein can result in selective subsequent damage to other proteins, as evidenced by loss of enzymatic activity, via the formation and subsequent reactions of protein peroxides. These reactions may be important in the development of cellular dysfunction as a result of photo-oxidation.

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“…and Stoppani, 1997). GR is known to be an essential enzyme in regulation of overall homeostatic oxido-reductive balance in any living cell, and the lack of GR activity was previously demonstrated in numerous clinical pathologies (Loos et al, 1976).…”

Section: Q8mentioning

confidence: 99%

l-2-oxothiazolidine-4-carboxylate influence on age- and heat exposure-dependent peroxidation in rat's liver and kidney

Hadzi‐Petrushev

Јankulovski

Milev

et al. 2012

Journal of Thermal Biology

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Inactivation of Yeast Glutathione Reductase by Fenton Systems: Effect of Metal Chelators, Catecholamines and Thiol Compounds

Cited by 41 publications

References 42 publications

Ischemia Modified Albumin Is a Sensitive Marker of Myocardial Ischemia After Percutaneous Coronary Intervention

Ischemia Modified Albumin Is a Sensitive Marker of Myocardial Ischemia After Percutaneous Coronary Intervention

Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack

l-2-oxothiazolidine-4-carboxylate influence on age- and heat exposure-dependent peroxidation in rat's liver and kidney

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