Inactivation of viruses by chemically and photochemically generated singlet molecular oxygen

Photodynamic inactivation of Escherichia coli with cationic meso-tetraarylporphyrins – The charge number and charge distribution effects

“…It has been extensively confirmed that the generation of singlet oxygen is essential for the photodynamic process [46][47][48]. However, it is not the only factor that determines the overall PDI efficiency.…”

SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer

“…Viral structures, such as the envelope or the capsid, can be targets of photodynamic inactivation leading to loss of infectivity (Müller-Breitkreutz et al, 1995) by inhibiting the adhesion of the virus to the host cell and its penetration (O'Brien et al, 1992; or impeding viral replication by inducing damages into viral nucleic acids (Specht, 1994). Due to the short half-life and high reactivity of ROS, especially of singlet oxygen, the main targets of photodynamic inactivation are the microbial external structures (Ben-Hur et al, 1992;Malik et al, 1993;Schneider et al, 1998;Smetana et al, 1998;Gábor et al, 2001;Egyeki et al, 2003;Floyd et al, 2004;Hotze et al, 2009).…”

11-Tungstophosphate with Iron(II) and Hydrogen Peroxide Efficiently Detached Bacterial Biofilm

“…According to the manufacturer's technical inserts, NDP was activated to NDPO 2 by using Na 2 MoO 4 and H 2 O 2 at pH 10. 14) A superoxide anion (O 2 Ϫ )-specific probe (4,5-dimethoxy-2-nitrobenzenesulfonyl tetrafluorofluorescein (BES-So)) was purchased from Wako Pure Chemicals. 15) Since it was supplied as an acetoxymethyl ester of BES-So (inactive) to improve cell permeability, this part was removed by esterase (1 U/ml) before use.…”

“…In addition, the amount of NDPO 2 added to the reaction mixture was corrected by its purity (ca. 90%, determined photometrically), 14) but 1 O 2 yield was reported to be only ca. 50%.…”