Inactivation of Vibrio parahaemolyticus by Aqueous Ozone

Abstract

Vibrio parahaemolyticus contamination, causes serious foodborne illness, and it has become a global health problem. As a disinfectant, aqueous ozone can effectively kill a number of bacteria, viruses, parasites and other microorganisms. In this study, three factors, namely, the aqueous ozone concentration, the exposure time, and the bacterial density, were analyzed by response surface methodology, and the aqueous ozone concentration was the most influential factor in the sterilization ratio. Under low aqueous ozone concentrations (less than 0.125 mg/L), the bacterial cell membranes remained intact, and the ozone was detoxified by intracellular antioxidant enzymes (e.g., superoxide dismutase and catalase). Under high aqueous ozone concentrations (more than 1 mg/L), cell membranes were damaged by the degree of peripheral electronegativity at the cell surface and the concentration of lactate dehydrogenase (LDH) released into the extracellular space, and the ultra-structures of the cells were confirmed by transmission electron microscopy (TEM). Aqueous ozone penetrated the cells through leaking membranes, inactivated the enzymes, inhibited almost all the genes, and degraded the genetic materials of gDNA and total RNA, which eventually lead to cell death.

Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

“…Studies based on prokaryotic transcriptome sequencing have included E. coli (Tao H 1999), group A Streptococcus (Smoot LM 2001), S. aureus (Tan X 2015), and Listeria monocytogenes (Pieta L 2017). Moreover, the difference in gene expression of V. parahaemolyticus in seawater has been studied under different oxygen environments (Feng L 2018). Therefore, RNA sequencing was applied in this study to understand the corresponding changes of V. parahaemolyticus CGMCC 1.1614 under the action of 1/8 MIC BITC.…”

“…Recent ndings have con rmed that agella are adhesive and invasive as a potential class of virulence factors (Johanna H 2013). Bacterial motility is closely related to agella, and V. parahaemolyticus has two different types of agella to accommodate life in different situations (Feng L 2018). Studies have shown that bacterial motility is also inhibited when agella are affected.…”

Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

“…Studies based on prokaryotic transcriptome sequencing have included E. coli [ 21 ], S. aureus [ 22 ], and Listeria monocytogenes [ 23 ]. Moreover, the difference in gene expression of V. parahaemolyticus in seawater has been studied under different oxygen environments [ 24 ]. Therefore, RNA sequencing was applied in this study to understand the corresponding changes of V. parahaemolyticus CGMCC 1.1614 under the action of 1/8 MIC BITC.…”

Quality of vacuum packaged beef as affected by aqueous ozone and sodium citrate treatment

“…[8] Aqueous ozone (AO) is a disinfectant that can effectively kill a large number of bacteria, parasites, viruses, and other microorganisms through membrane leakage, inactivation of enzymes, inhibition of almost all genes, degradation of gDNA, and total RNA genetic material, ultimately leading to cell death. [9] Sodium citrate (SC) has been evaluated for antioxidative effects and could inhibit various microorganisms. [10] Ponrajan et al have found that SC plus sodium diacetate or buffered vinegar have good antimicrobial effects.…”