Inactivation of transforming DNA by ultraviolet light I. Near-UV action spectrum for marker inactivation

Peak, Meyrick J.; Peak, Jennifer G.; Webb, Robert B.

doi:10.1016/0027-5107(73)90182-6

Cited by 36 publications

(1 citation statement)

References 23 publications

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“…This observation is consistent with the fact that BL-inactivated transforming DNA is not photoreactivable by Neurospora enzyme extracts, whereas UV-inactivated DNA is (Tables 1 and 2). Peak et al (12)(13)(14) have suggested that near-UV and shortwave UV inactivation of transforming DNA occur via different mechanisms. However, one can argue that pyrimidine dimers are induced in the DNA of N. crassa conidia during BL irradiation but that inactivation and maximal PR occur concomitantly so that no recovery is observed with subsequent PR treatment (1).…”

Section: Resultsmentioning

confidence: 99%

Inactivation of carotenoid-producing and albino strains of Neurospora crassa by visible light, blacklight, and ultraviolet radiation

Blanc¹,

Tuveson²,

Sargent³

1976

J Bacteriol

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Suspensions of Neurospora crassa conidia were inactivated by blacklight (BL) radiation (300 to 425 nm) in the absence of' exogenous photosensitizing compounds. Carotenoid-containing wild-type conidia were less sensitive to BL radiation than albino conidia, showing a dose enhancement factor (DEF) of 1.2 for dose levels resulting in less than 10%. survival. The same strains were about equally sensitive to shortwave ultraviolet (UV) inactivation. The kinetics of BL inactivation are similar to those of photodynamic inactivation by visible light in the presence of a photosensitizing dye (methylene blue). Only limited inactivation by visible light in the absence of exogenous photosensitizers was observed. BL and UV inactivations are probably caused by different mechanisms since wild-type conidia are only slightly more resistant to BL radiation (DEF = 1.2 at 1.0% survival) than are conidia from a UV-sensitive strain (upr-1, uvs-3). The BL-induced lethal lesions are probably not cyclobutyl pyrimidine dimers since BL-inactivated Haemophilus influenzae transforming deoxyribonucleic acid is not photoreactivated by N. crassa wild-type enzyme extracts, whereas UV-inactivated transforming deoxyribonucleic acid is photoreactivable with this treatment.

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Section: Resultsmentioning

confidence: 99%