Inactivation of the particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath) by acetylene

Abstract

Inactivation of the particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath) by acetylene, BBA -Proteins and Proteomics (2015), doi: 10.1016/j.bbapap.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. experiments with two related alkynes: propyne (CH 3 CCH) and trifluoropropyne (CF 3 CCH). Finally, we discuss the implication of these findings to the location of the catalytic site in pMMO. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

A tale of two methane monooxygenases

“…Copper presumably binds at the location of the crystallographically modeled dicopper active site since mutation of the histidine ligands diminishes metal binding and completely abrogates activity [31], and EXAFS data indicate the presence of a short Cu–Cu distance as observed in pMMO [31]. These data also rule out the PmoC metal center and a proposed tricopper center in PmoA [116, 117] as the active site since these subunits are not present in spmoB.…”

“…Methane to methanol conversion by tricopper complexes and zeolites containing tricopper species has also been reported [152–156]. However, no copper has been observed at the site of the proposed PmoA tricopper active site [116, 117], and the exact electronic structure of the species giving rise to an EPR signal assigned to a tricopper site [116, 124, 125] remains unclear [106, 157–159]. …”

The PmoB subunit of particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath): The CuI sponge and its function

“…8 the PmoA and PmoC subunits in the transmembrane domain of the protein complex [13,19,24], we can begin to understand the role played by these copper ions of the PmoB subunit in the catalytic cycle of the holo enzyme.…”

“…PmoA and PmoC [19,24]. The three copper ions forming the tricopper cluster were lost during the purification of the protein for crystallization and X-ray analysis [3,6,13].…”

Synergistic activating effect of promoter and oxidant in single step conversion of methane into methanol over a tailored polymer-Ag coordination complex

“…24 The promoter's role is to activate CH 4 by reducing the bond dissociation energy of the C-H bond. Halide anions are well known as CH 4 activators, and scientists have made use of alkyl halides and halide salts for this purpose.…”