Inactivation of the gene katA or sodA affects the transient entry into the viable but non-culturable response of Staphylococcus aureus in natural seawater at low temperature

Masmoudi, Salma; Denis, Michel; Maâlej, Sami

doi:10.1016/j.marpolbul.2010.08.017

Cited by 50 publications

(21 citation statements)

References 40 publications

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“…Previous research demonstrates the importance of superoxide dismutase in Salmonella and E. coli exposed to reactive oxygen species such as hydrogen peroxide [47], N. gonorrhoeae exposed to peroxides [25], and Staphlococcus aureus in seawater [59]. In the present study, superoxide dismutase in E. faecalis exposed to sunlight for 12 h increased in abundance (fc = 2.04 and FDR = 19%).…”

Section: Differential Expression Of Genes Involved In Bacterial Stressupporting

confidence: 65%

Transcriptional response of Enterococcus faecalis to sunlight

Sassoubre

Ramsey

Gilmore

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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Section: Differential Expression Of Genes Involved In Bacterial Stressupporting

confidence: 65%

Transcriptional response of Enterococcus faecalis to sunlight

Sassoubre

Ramsey

Gilmore

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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“…Kong et al (6) found that cold-induced loss of culturability in Vibrio vulnificus occurred concomitantly with the loss of catalase activity. This phenomenon was also observed in VBNC Staphylococcus aureus (7). Asakura et al (8) reported that negative modulation of RNA polymerase sigma S (RpoS) expression could promote the VBNC state formation in E. coli O157:H7 under conditions of osmotic and oxidative stresses.…”

Section: Introductionsupporting

confidence: 57%

“…But the VBNC cells were also more susceptible to oxidative stress than the control cells. Kong et al (6) and Masmoudi et al (7) found that loss of culturability in V. vulnificus and S. aureus occurred concomitantly with the loss of catalase activity. Meanwhile, Serpaggi et al (10) proposed that oxidative stress would be a driving factor for VBNC state entry.…”

Section: Resultsmentioning

confidence: 98%

New Insights into the Formation of Viable but Nonculturable Escherichia coli O157:H7 Induced by High-Pressure CO ₂

Zhao

Wang

et al. 2016

mBio

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The formation of viable but nonculturable (VBNC) Escherichia coli O157:H7 induced by high-pressure CO2 (HPCD) was investigated using RNA sequencing (RNA-Seq) transcriptomics and isobaric tag for relative and absolute quantitation (iTRAQ) proteomic methods. The analyses revealed that 97 genes and 56 proteins were significantly changed upon VBNC state entry. Genes and proteins related to membrane transport, central metabolisms, DNA replication, and cell division were mainly downregulated in the VBNC cells. This caused low metabolic activity concurrently with a division arrest in cells, which may be related to VBNC state formation. Cell division repression and outer membrane overexpression were confirmed to be involved in VBNC state formation by homologous expression of z2046 coding for transcriptional repressor and ompF encoding outer membrane protein F. Upon VBNC state entry, pyruvate catabolism in the cells shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route; this led to a low level of ATP. Combating the low energy supply, ATP production in the VBNC cells was compensated by the degradation of l-serine and l-threonine, the increased AMP generation, and the enhanced electron transfer. Furthermore, tolerance of the cells with respect to HPCD-induced acid, oxidation, and high CO2 stresses was enhanced by promoting the production of ammonia and NADPH and by reducing CO2 production during VBNC state formation. Most genes and proteins related to pathogenicity were downregulated in the VBNC cells. This would decrease the cell pathogenicity, which was confirmed by adhesion assays. In conclusion, the decreased metabolic activity, repressed cell division, and enhanced survival ability in E. coli O157:H7 might cause HPCD-induced VBNC state formation.

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“…For example, the major foodborne pathogens, such as E. coli O157, C. jejuni, V. parahaemolyticus, L. monocytogenes , and S. aureus , have been validated to enter the VBNC state under low temperatures conditions (Bates and Oliver, 2004; Masmoudi et al, 2010; Chaisowwong et al, 2012; Dinu and Bach, 2013; Patrone et al, 2013; Gião and Keevil, 2014; Liu et al, 2016). In addition to low temperature factors, cells entering the VBNC state have been detected during high temperature sterilization processes such as the pasteurization of milk (Gunasekera et al, 2002).…”

Section: Induction Of Vbnc Cellsmentioning

confidence: 99%

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

et al. 2017

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The viable but non-culturable (VBNC) state, a unique state in which a number of bacteria respond to adverse circumstances, was first discovered in 1982. Unfortunately, it has been reported that many foodborne pathogens can be induced to enter the VBNC state by the limiting environmental conditions during food processing and preservation, such as extreme temperatures, drying, irradiation, pulsed electric field, and high pressure stress, as well as the addition of preservatives and disinfectants. After entering the VBNC state, foodborne pathogens will introduce a serious crisis to food safety and public health because they cannot be detected using conventional plate counting techniques. This review provides an overview of the various features of the VBNC state, including the biological characteristics, induction and resuscitation factors, formation and resuscitation mechanisms, detection methods, and relationship to food safety.

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Inactivation of the gene katA or sodA affects the transient entry into the viable but non-culturable response of Staphylococcus aureus in natural seawater at low temperature

Cited by 50 publications

References 40 publications

Transcriptional response of Enterococcus faecalis to sunlight

Transcriptional response of Enterococcus faecalis to sunlight

New Insights into the Formation of Viable but Nonculturable Escherichia coli O157:H7 Induced by High-Pressure CO ₂

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

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Inactivation of the gene katA or sodA affects the transient entry into the viable but non-culturable response of Staphylococcus aureus in natural seawater at low temperature

Cited by 50 publications

References 40 publications

Transcriptional response of Enterococcus faecalis to sunlight

Transcriptional response of Enterococcus faecalis to sunlight

New Insights into the Formation of Viable but Nonculturable Escherichia coli O157:H7 Induced by High-Pressure CO 2

Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens

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New Insights into the Formation of Viable but Nonculturable Escherichia coli O157:H7 Induced by High-Pressure CO ₂