Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host

Pinedo, Francisco; Moscardi, F.; Luque, Teresa; Olszewski, Julie A.; Ribeiro, Bergmann Morais

doi:10.1016/s1049-9644(03)00026-4

Cited by 33 publications

(28 citation statements)

References 39 publications

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“…Toxin genes isolated from other scorpions, for example Leiurus quinquestriatus hebraeus (Imai et al, 2000), straw itch mite Pyemotes tritici (Burden et al, 2000), ants (Szolajska et al, 2004) or spiders (Prikhod'ko Prikhod'ko et al, 1998), sea anemones and B. thuringiensis (Chang et al, 2003) have been intensively studied as potential enhancers of baculovirus activity. Genetically modified Anticarsia gemmatalis multicapsid nuclear polyhedrosis virus (AgMNPV) by the inactivation of ecdysteroid glucosyltransferase (egt) gene, were shown to kill infected larvae more rapidly when compared to wild-type virus infections (Pinedo et al, 2003). Ignoffo et al (1995) found that AcMNPV pp34 deletion mutants have an increased infectivity in Trichoplusia ni larvae due to the increased loads of occlusion-derived virions (ODV) from the polyhedra in the midgut to start the infection process.…”

Section: Recombinant Virusesmentioning

confidence: 99%

Microbes as interesting source of novel insecticides: A review

Kumari¹,

Vijayabharathi²,

Srinivas³

et al. 2014

Afr. J. Biotechnol.

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Microbes are ubiquitous, survive in all sorts of environments and have a profound influence on the earth. In the present day plant protection scenario, development of resistance to chemical pesticides is the major hurdle in insect pest management. In recent years, several microbes with potential insecticidal properties have come to light. Viruses, bacteria, fungi and protozoa that are known to produce an array of metabolites or toxins, form the basis for microbial insecticides. Since these versatile organisms are amenable for genetic engineering, strains with good insecticidal properties can be identified, evaluated and utilized for pest control. This paper reviews the insecticidal properties of microbes and their potential utility in pest management.

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Section: Recombinant Virusesmentioning

confidence: 99%

Microbes as interesting source of novel insecticides: A review

Kumari¹,

Vijayabharathi²,

Srinivas³

et al. 2014

Afr. J. Biotechnol.

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“…Efforts have been made to explore AgMNPV molecular biology and host interaction with the attempt to enhance the economical and scientific application of this virus ( Pombo et al, 1998, Matos et al, 1999, Silveira et al, 1999, Castro and Ribeiro, 2001, Rodrigues et al, 2001, Razuck et al, 2002and Pinedo et al, 2003.…”

Section: Introductionmentioning

confidence: 99%

Morphological characterization of Anticarsia gemmatalis M nucleopolyhedrovirus infection in haemocytes from its natural larval host, the velvet bean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

2004

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For a better understanding of virus×host interactions, transmission electron microscopy was used to characterize the intrahaemocoelic infection of Anticarsia gemmatalis larval haemocytes by A. gemmatalis M nucleopolyhedrovirus (AgMNPV). At 12 h post-infection (h p.i.), we observed nuclear hypertrophy, budded virus assembling, and protrusion towards the cytoplasm, virion envelopment, and accumulation of fibrillar aggregates in the cytoplasm. Around 24 h p.i., fibrillar aggregates also appeared inside nuclei of infected cells. By 48 h p.i., virogenic stroma and polyhedra were visualised in nuclei and at 72 h p.i., widespread infection in haemocytes was observed. Cell remnants and free polyhedra were phagocytosed by granular haemocyte 1 and plasmatocytes. Entire cells were phagocytosed only by plasmatocytes. Necrosis of infected cells was quite common, suggesting a putative cytotoxic response. Granular haemocyte 1 presented a more exuberant protrusion of budded viruses in comparison to other haemocytes. All types of haemocytes were shown to be infected, and the intense virus replication in some of these cells reveals the importance of haemolymph for AgMNPV spread in its natural host, a critical factor for permissiveness.

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“…Many baculoviruses sequenced to date have been shown to contain egt gene that encodes for an egt enzyme (Ahrens et al 1997, Ijkel et al 1999, Kuzio et al 1999 and this enzyme inactivates insect ecdysteroid hormones (Bianchi et al 2000, O'Reilly andMiller 1989). This inactivation leads to a cessation of metamorphosis, keeping the insect in its larval, voracious state and therefore increasing the production of viral progeny (Pinedo et al 2003). Briefly, presence of sufficient quantities of egt enzyme delays or prevents molting but in some cases, there can be a balance between the ecdysone concentration excreted by the larvae and egt concentration.…”

Section: Resultsmentioning

confidence: 99%

Gross Pathology of SpliNPVs and Alterations in Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) Morphology Due to Baculoviral Infection*

;BAYRAM¹

2005

Tarım Bilimleri Dergisi

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Baculoviruses are invertebrate-specific pathogens and baculoviral infections cause alterations in the physiology, metabolism and morphology of insects. It is important to recognize these physiological and symptomatologic changes to understand baculovirus infection cycle and biology. For this reason, in our study Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae) larvae were inoculated with the SpliNPV to doses of 3000 and 20,000 OBs for third instars and to concentrations of 10 6 and 3x 10 6 OBs/ml for neonates and the alterations due to NPV infection in larvae were then examined. Bioassays carried out with the third instar S. littoralis larvae revealed that no symptoms were detected during the first three days post-inoculation due to SpliNPV infection. Approximately, in the 4th day, infected larvae began to respond much more slowly than healthy larvae. This symptom was followed by whitening and slimming of cuticula, failure in molting, swelling of the body, diarrhoea, climbing to high places and hanging, liquefaction of the body and death. Infected neonates exhibited limited symptoms of distension and the failure of molting. The larvae died in approximately 8-8.5 days for third instars and in approximately 3-3.5 days for neonates due to baculoviral infection for the both doses and concentrations. On the other hand, different doses or concentrations did not cause an alteration in the occurence time of symptoms. To associate the NPV biology with the genes involved in baculovirus genome and understand their life cycle will improve their efficacy as biopesticides and help to the effective use of baculoviruses. 6 and 3x 10 6 OBs/ml konsantrasyonlarında SpliNPV ile inokule edilmiş ve daha sonra larvalarda NPV enfeksiyonundan kaynaklanan değişimler irdelenmiştir. Üçüncü dönem S. littoralis larvalarıyla yapılan denemelerde inokulasyonu takip eden ilk üç gün boyunca SpliNPV enfeksiyonundan kaynaklı simptom görülmediği saptanmıştır. Yaklaşık olarak dördüncü günle birlikte hastalıklı larvalar sağlıklı larvalara göre daha yavaş tepkiler vermiştir. Bu simptomu, kutikulanın beyazlaşması ve incelmesi, deri değiştirememe, vücudun şişmesi, ishal durumu, yüksek yerlere tırmanma ve asma, vücudun sıvılaşması ve ölüm takip etmiştir. Enfekteli birinci dönem larvalar ise şişme ve deri değiştirememeyi içeren kısıtlı simptomlar vermiştir. Bakulovirüs enfeksiyonundan dolayı 3. dönem larvalar 8-8.5 günde, 1. dönem larvalar ise 3-3.5 günde her iki doz ve konsantrasyonda ölmüştür. Diğer taraftan farklı doz ya da konsantrasyonlar, simptomların ortaya çıkış zamanında bir değişikliğe neden olmamıştır. NPV bioyolojisini bakulovirus genomunda yer alan genlerle ilişkilendirmek ve hayat döngülerini anlamak bunların birer biyopestisit olarak etkinliğini artıracak ve daha etkin kullanımlarına yardımcı olacaktır.

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Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host

Cited by 33 publications

References 39 publications

Microbes as interesting source of novel insecticides: A review

Microbes as interesting source of novel insecticides: A review

Morphological characterization of Anticarsia gemmatalis M nucleopolyhedrovirus infection in haemocytes from its natural larval host, the velvet bean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

Gross Pathology of SpliNPVs and Alterations in Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) Morphology Due to Baculoviral Infection*

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