Inactivation of Staphylococcus aureus by photodynamic action of hypocrellin B

Jiang, Yuan; Leung, Albert Wingnang; Wang, Xinna; Zhang, Hongwei; Xu, Chuanshan

doi:10.1016/j.pdpdt.2013.06.004

Cited by 48 publications

(36 citation statements)

References 21 publications

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“…ROS levels increased substantially in cells treated with PDI. The treatment caused reduction of viability as demonstrated by CFU counts and cellular damage, including membrane damage and cytoplasm leakage that was corroborated by confocal laser scanning microscopy [73]. …”

Section: Application Of Flow Cytometrymentioning

confidence: 88%

“…Since PDI causes nonspecific injuries to the pathogens, it is very unlikely that bacteria can acquire resistance. One work related to ROS production by photoactivation of hypocrellin B (a component of a traditional Chinese herb H. bambuase ) was reported by Jiang et al [73]. In this report, clinically isolated S. aureus cells were incubated with hypocrellin B and after photodynamic treatment they used DCFH-DA (dichlorodihydrofluorescein diacetate) to measure by FCM the production of ROS [74].…”

Section: Application Of Flow Cytometrymentioning

confidence: 99%

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Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Ambriz-Aviña

Contreras‐Garduño

Pedraza-Reyes

2014

BioMed Research International

122

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Although reports of flow cytometry (FCM) applied to bacterial analysis are increasing, studies of FCM related to human cells still vastly outnumber other reports. However, current advances in FCM combined with a new generation of cellular reporter probes have made this technique suitable for analyzing physiological responses in bacteria. We review how FCM has been applied to characterize distinct physiological conditions in bacteria including responses to antibiotics and other cytotoxic chemicals and physical factors, pathogen-host interactions, cell differentiation during biofilm formation, and the mechanisms governing development pathways such as sporulation. Since FCM is suitable for performing studies at the single-cell level, we describe how this powerful technique has yielded invaluable information about the heterogeneous distribution of differently and even specialized responding cells and how it may help to provide insights about how cell interaction takes place in complex structures, such as those that prevail in bacterial biofilms.

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Section: Application Of Flow Cytometrymentioning

confidence: 88%

Section: Application Of Flow Cytometrymentioning

confidence: 99%

Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Ambriz-Aviña

Contreras‐Garduño

Pedraza-Reyes

2014

BioMed Research International

122

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“…In the USA, the estimated annual number of CAP episodes in adults is approximately 5.2 million, with the highest incidence among those aged 65 years and older (Jiang et al. ).…”

Section: Introductionmentioning

confidence: 99%

Pneumonia treatment by photodynamic therapy with extracorporeal illumination ‐ an experimental model

Geralde

Leite

Inada

et al. 2017

Physiological Reports

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Infectious pneumonia is a major cause of morbidity/mortality, mainly because of the increasing rate of microorganisms resistant to antibiotics. Photodynamic Therapy (PDT) is emerging as a promising approach, as effects are based on oxidative stress, preventing microorganism resistance. In two previous studies, the in vitro inactivation of Streptococcus pneumoniae using indocyanine green (ICG) and infrared light source was a success killing 5 log10 colony‐forming units (CFU/mL) with only 10 μmol/L ICG. In this work, a proof‐of‐principle protocol was designed to treat lung infections by PDT using extracorporeal illumination with a 780 nm laser device and also ICG as photosensitizer. Hairless mice were infected with S. pneumoniae and PDT was performed two days after infection. For control groups, CFU recovery ranged between 103–104/mouse. For PDT group, however, no bacteria were recovered in 80% of the animals. Based on this result, animal survival was evaluated separately over 50 days. No deaths occurred in PDT group, whereas 60% of the control group died. Our results indicate that extracorporeal PDT has the potential for pneumonia treatment, and pulmonary decontamination with PDT may be used as a single therapy or as an antibiotics adjuvant.

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“…One of the newest advances in the treatment of different pathologies of microbial, viral, fungal and inflammatory origin is the application of photodynamic therapy (PDT) as antimicrobial activity [6]. This therapy consists in the use of a photosensitizer (PS) that, when activated by an electromagnetic radiation at a specific wavelength, can react with molecules in its vicinity.…”

Section: Introductionmentioning

confidence: 99%

“…The two reactions may lead to cell death or destruction of the tissue. Due to the nature of these mechanisms of action, it is highly unlikely that microorganisms acquire resistance to this technique [6,7].…”

Section: Introductionmentioning

confidence: 99%

Pulmonary decontamination for photodynamic inactivation with extracorporeal illumination

Geralde

Leite²,

Inada³

et al. 2014

SPIE Proceedings

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Infectious pneumonia is a major cause of morbidity and mortality, despite advances in diagnostics and therapeutics in pulmonary infections. One of the major difficulties associated with the infection comes from the high rate of antibiotic resistant microorganisms, claiming for the use of alternative techniques with high efficiency and low cost. The photodynamic inactivation (PDI) is emerging as one of the great possibilities in this area, once its action is oxidative, not allowing microorganism develops resistance against the treatment. PDI for decontamination pulmonary has potential for treatment or creating better conditions for the action of antibiotics. In this study, we are developing a device to implement PDI for the treatment of lung diseases with extracorporeal illumination. To validate our theory, we performed measurements in liquid phantom to simulate light penetration in biological tissues at various fluency rates, the temperature was monitored in a body of hairless mice and the measurements of light transmittance in this same animal model. A diode laser emitting at 810 nm in continuous mode was used. Our results show 70% of leakage at 0.5 mm of thickness in phantom model. The mouse body temperature variation was 5.4 °C and was observed light transmittance through its chest. These results are suggesting the possible application of the extracorporeal illumination using infrared light source. Based on these findings, further studies about photodynamic inactivation will be performed in animal model using indocyanine green and bacteriochlorin as photosensitizers. The pulmonary infection will be induced with Streptococcus pneumoniae and Klebsiella pneumoniae.

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Inactivation of Staphylococcus aureus by photodynamic action of hypocrellin B

Cited by 48 publications

References 21 publications

Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Pneumonia treatment by photodynamic therapy with extracorporeal illumination ‐ an experimental model

Pulmonary decontamination for photodynamic inactivation with extracorporeal illumination

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