Inactivation of Shiga toxin-producing Escherichia coli O104:H4 using cold atmospheric pressure plasma

Baier, Matthias; Janßen, Traute; Wieler, Lothar H.; Ehlbeck, Jörg; Knorr, Dietrich; Schlüter, Oliver

doi:10.1016/j.jbiosc.2015.01.003

Cited by 35 publications

(8 citation statements)

References 16 publications

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“…Similar to the treatment with ozonated water the CAPP showed different mode of actions against the tested bacteria whereas the greatest differences were detected between Gram-negative and Gram-positive bacteria. Different sensitivity of bacteria was also found by Baier et al ( 2015 ). They showed that CAPP treatment has a different impact on three E. coli serovars tested.…”

Section: Discussionsupporting

confidence: 71%

Flow cytometric evaluation of physico-chemical impact on Gram-positive and Gram-negative bacteria

Fröhling

Schlüter

2015

Front. Microbiol.

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Since heat sensitivity of fruits and vegetables limits the application of thermal inactivation processes, new emerging inactivation technologies have to be established to fulfill the requirements of food safety without affecting the produce quality. The efficiency of inactivation treatments has to be ensured and monitored. Monitoring of inactivation effects is commonly performed using traditional cultivation methods which have the disadvantage of the time span needed to obtain results. The aim of this study was to compare the inactivation effects of peracetic acid (PAA), ozonated water (O3), and cold atmospheric pressure plasma (CAPP) on Gram-positive and Gram-negative bacteria using flow cytometric methods. E. coli cells were completely depolarized after treatment (15 s) with 0.25% PAA at 10°C, and after treatment (10 s) with 3.8 mg l−1 O3 at 12°C. The membrane potential of CAPP treated cells remained almost constant at an operating power of 20 W over a time period of 3 min, and subsequently decreased within 30 s of further treatment. Complete membrane permeabilization was observed after 10 s O3 treatment, but treatment with PAA and CAPP did not completely permeabilize the cells within 2 and 4 min, respectively. Similar results were obtained for esterase activity. O3 inactivates cellular esterase but esterase activity was detected after 4 min CAPP treatment and 2 min PAA treatment. L. innocua cells and P. carotovorum cells were also permeabilized instantaneously by O3 treatment at concentrations of 3.8 ± 1 mg l−1. However, higher membrane permeabilization of L. innocua and P. carotovorum than of E. coli was observed at CAPP treatment of 20 W. The degree of bacterial damage due to the inactivation processes is highly dependent on treatment parameters as well as on treated bacteria. Important information regarding the inactivation mechanisms can be obtained by flow cytometric measurements and this enables the definition of critical process parameters.

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Section: Discussionsupporting

confidence: 71%

Flow cytometric evaluation of physico-chemical impact on Gram-positive and Gram-negative bacteria

Fröhling

Schlüter

2015

Front. Microbiol.

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“…; Baier et al . ). With regard to moisture content, 17% was found to be an optimum for the decontamination of seeds, as compared to either 8 or 30% (Butscher et al .…”

Section: Inactivation Of Food‐borne Pathogenic Microorganismsmentioning

confidence: 97%

“…Argon plasma treatment for 1 min reduced E. coli O157:H7 levels on the surface of corn salad leaves by 3·3 log, whereas 2 min of treatment was required to reduce E. coli O104:H4 to similar levels (Baier et al . ).…”

Section: Inactivation Of Food‐borne Pathogenic Microorganismsmentioning

confidence: 97%

Microbiological interactions with cold plasma

Bourke¹,

Ziuzina²,

Han³

et al. 2017

J Appl Microbiol

292

213

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SummaryThere is a diverse range of microbiological challenges facing the food, healthcare and clinical sectors. The increasing and pervasive resistance to broad-spectrum antibiotics and health-related concerns with many biocidal agents drives research for novel and complementary antimicrobial approaches. Biofilms display increased mechanical and antimicrobial stability and are the subject of extensive research. Cold plasmas (CP) have rapidly evolved as a technology for microbial decontamination, wound healing and cancer treatment, owing to the chemical and bio-active radicals generated known collectively as reactive oxygen and nitrogen species. This review outlines the basics of CP technology and discusses the interactions with a range of microbiological targets. Advances in mechanistic insights are presented and applications to food and clinical issues are discussed. The possibility of tailoring CP to control specific microbiological challenges is apparent. This review focuses on microbiological issues in relation to food-and healthcareassociated human infections, the role of CP in their elimination and the current status of plasma mechanisms of action.

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“…Atmospheric-pressure non-thermal plasma has demonstrated antibacterial potential by inhibiting and reducing the growth of bacteria (Mai-Prochnow et al, 2014;Gilmore et al, 2018). The majority of studies have been focused on food-contaminating and -poisoning bacteria, such as Escherichia coli, Staphylococcus aureus, Listeria, and Salmonella (Baier et al, 2013;Sun et al, 2014;Baier et al, 2015;Matan et al, 2015). Compared to that, the treatment of bacteria causing plant diseases has been relatively less explored in the application of plasma ( Table 1).…”

Section: Bacterial Pathogensmentioning

confidence: 99%

Plant Disease Control by Non-Thermal Atmospheric-Pressure Plasma

et al. 2020

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Disease stresses caused by pathogenic microorganisms are increasing, probably because of global warming. Conventional technologies for plant disease control have often revealed their limitations in efficiency, environmental safety, and economic costs. There is high demand for improvements in efficiency and safety. Non-thermal atmospheric-pressure plasma has demonstrated its potential as an alternative tool for efficient and environmentally safe control of plant pathogenic microorganisms in many studies, which are overviewed in this review. Efficient inactivation of phytopathogenic bacterial and fungal cells by various plasma sources under laboratory conditions has been frequently reported. In addition, plasma-treated water shows antimicrobial activity. Plasma and plasma-treated water exhibit a broad spectrum of efficiency in the decontamination and disinfection of plants, fruits, and seeds, indicating that the outcomes of plasma treatment can be significantly influenced by the microenvironments between plasma and plant tissues, such as the surface structures and properties, antioxidant systems, and surface chemistry of plants. More intense studies are required on the efficiency of decontamination and disinfection and underlying mechanisms. Recently, the induction of plant tolerance or resistance to pathogens by plasma (so-called "plasma vaccination") is emerging as a new area of study, with active research ongoing in this field.

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Inactivation of Shiga toxin-producing Escherichia coli O104:H4 using cold atmospheric pressure plasma

Cited by 35 publications

References 16 publications

Flow cytometric evaluation of physico-chemical impact on Gram-positive and Gram-negative bacteria

Flow cytometric evaluation of physico-chemical impact on Gram-positive and Gram-negative bacteria

Microbiological interactions with cold plasma

Plant Disease Control by Non-Thermal Atmospheric-Pressure Plasma

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