Inactivation of prions by physical and chemical means

Taylor, D. M.

doi:10.1016/s0195-6701(99)90067-1

Cited by 181 publications

(118 citation statements)

References 30 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…Drawbacks in actual sterilization processes are related in particular to the high resistance of certain biomolecules, for instance prions, towards routine chemical and physical cleaning processes [3] or incompatibility between the materials used in biomedical applications and the process parameters [4][5][6]. Infectious proteins, i.e.…”

Section: Introductionmentioning

confidence: 99%

Low-pressure water vapour plasma treatment of surfaces for biomolecules decontamination

Fumagalli¹,

Kylián²,

Amato³

et al. 2012

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

Decontamination treatments of surfaces are performed on bacterial spores, albumin and brain homogenate used as models of biological contaminations in a low-pressure, inductively coupled plasma reactor operated with water-vapour-based gas mixtures. It is shown that removal of contamination can be achieved using pure H 2 O or Ar/H 2 O mixtures at low temperatures with removal rates comparable to oxygen-based mixtures. Particle fluxes (Ar + ions, O and H atomic radicals and OH molecular radicals) from water vapour discharge are measured by optical emission spectroscopy and Langmuir probe under several operating conditions. Analysis of particle fluxes and removal rates measurements illustrates the role of ion bombardment associated with O radicals, governing the removal rates of organic matter. Auxiliary role of hydroxyl radicals is discussed on the basis of experimental data. The advantages of a water vapour plasma process are discussed for practical applications in medical devices decontamination.

show abstract

Section: Introductionmentioning

confidence: 99%

Low-pressure water vapour plasma treatment of surfaces for biomolecules decontamination

Fumagalli¹,

Kylián²,

Amato³

et al. 2012

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

show abstract

“…The high resistance of TSE agents to conventional methods of chemical or thermal inactivation and to UV or ionizing radiation (Alper et al, 1966(Alper et al, , 1967Brown et al, 1982Brown et al, , 1986Kimberlin et al, 1983;Taguchi et al, 1991;Tateishi et al, 1991;Ernst & Race, 1993;Taylor et al, 1994;Manuelidis, 1997;Taylor, 1999; for review see Taylor, 2000), as well as their high binding affinity to and tenacity on steel surfaces (Zobeley et al, 1999;Flechsig et al, 2001), warrant specific decontamination procedures in the reprocessing of surgical instruments (Rutala & Weber, 2001). Treatments that are considered appropriate for decontamination include use of 1-2 M NaOH solution (for 24 h), 2?5-5 % NaOCl solution (for 24 h) as well as 3, 4 or 6 M GdnSCN solution (for 24 h, 1 h or 15 min, respectively), followed by steam sterilization at 134 u C for 18 min to 1 h (Simon & Pauli, 1998;World Health Organization, 1999;Hörnlimann et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Decontamination of surgical instruments from prion proteins: in vitro studies on the detachment, destabilization and degradation of PrPSc bound to steel surfaces

Lemmer

Mielke

Pauli

et al. 2004

Journal of General Virology

View full text Add to dashboard Cite

Effective reprocessing of surgical instruments ensuring elimination of inadvertent contamination with infectious agents causing transmissible spongiform encephalopathies (TSEs) is essential for the prevention of iatrogenic transmission of Creutzfeldt-Jakob disease (CJD) or its new variant (vCJD) from asymptomatic carriers. In a search for effective yet instrument-friendly and routinely applicable reprocessing procedures, we used an in vitro carrier assay to assess the decontamination activity exerted by different reagents on pathological prion protein (PrP Sc ), the biochemical marker for TSE infectivity, attached to steel surfaces. In this assay, steel wires were contaminated with 263K scrapie brain homogenate and reprocessed for decontamination by exposure to several different test reagents. Residual contamination with PrP Sc and its protease-resistant core PrP27-30, still present after reprocessing on the wire surface or in the cleaning solution, was monitored by sensitive Western blot detection without or after proteinase K digestion. Using this approach, various reagents and processing conditions were screened for both their efficacy of decontamination and their active principles, such as detachment, destabilization or degradation of surface-bound prion protein. This revealed that, under appropriate conditions, relatively mild reagents such as 0?2 % SDS/0?3 % NaOH (pH 12?8), a commercially available alkaline cleaner (pH 11?9-12?2), a disinfectant containing 0?2 % peracetic acid and low concentrations of NaOH (pH 8?9) or 5 % SDS (pH 7?1) exert potent decontaminating activities on PrP Sc /PrP27-30 attached to steel surfaces. For in vivo validation, wires reprocessed in these reagents have been implanted into reporter animals in ongoing experiments.

show abstract

“…18 It is, therefore, important that the dental profession gives serious thought to provision of safe dental care, in the light of the resistance of prions to traditional sterilisation procedures. 5 The findings of the present study suggest that general dental practice is not currently equipped to address some of these issues.…”

Section: Discussionmentioning

confidence: 70%

Cross infection control measures and the treatment of patients at risk of Creutzfeldt Jakob Disease in UK general dental practice

Bagg¹,

Sweeney²,

Roy³

et al. 2001

Br Dent J

View full text Add to dashboard Cite

Inactivation of prions by physical and chemical means

Cited by 181 publications

References 30 publications

Low-pressure water vapour plasma treatment of surfaces for biomolecules decontamination

Low-pressure water vapour plasma treatment of surfaces for biomolecules decontamination

Decontamination of surgical instruments from prion proteins: in vitro studies on the detachment, destabilization and degradation of PrPSc bound to steel surfaces

Cross infection control measures and the treatment of patients at risk of Creutzfeldt Jakob Disease in UK general dental practice

Contact Info

Product

Resources

About