Inactivation of Methionine Synthetase by Nitrous Oxide in Mice

Koblin, Donald D.; Watson, J. E.; Deady, Joan E.; Stokstad, E. L. R.; Eger, Edmond I.

doi:10.1097/00000542-198104000-00012

Cited by 90 publications

(26 citation statements)

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“…The homogenates were centrifuged at 20,000g for 30 min at 4°C, and the supernatant was removed and used immediately to measure the activities of five of the enzymes of methionine metabolism. Methionine adenosyltransferase (MAT) (13,14), CBS (13,15), cystathionine ␥-lyase (CGL) (16), methionine synthase (17), and BHMT (18) were assayed using previously described methods. Methylenetet-rahydrofolate reductase (MTHFR) (19) and glycine N-methyltransferase (GNMT) (20) were measured using previously frozen liver tissue.…”

Section: Methodsmentioning

confidence: 99%

Homocysteine Metabolism in ZDF (Type 2) Diabetic Rats

et al. 2005

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Mild hyperhomocysteinemia is a risk factor for many diseases, including cardiovascular disease. We determined the effects of insulin resistance and of type 2 diabetes on homocysteine (Hcy) metabolism using Zucker diabetic fatty rats (ZDF/Gmi fa/fa and ZDF/Gmi fa/?). Plasma total Hcy was reduced in ZDF fa/fa rats by 24% in the prediabetic insulin-resistant stage, while in the frank diabetic stage there was a 59% reduction. Hepatic activities of several enzymes that play a role in the removal of Hcy: cystathionine ␤-synthase (CBS), cystathionine ␥-lyase, and betaine:Hcy methyltransferase (BHMT) were increased as was methionine adenosyltransferase. CBS and BHMT mRNA levels and the hepatic level of S-adenosylmethionine were also increased in the ZDF fa/fa rats. Studies with primary hepatocytes showed that Hcy export and the transsulfuration flux in cells from ZDF fa/fa rats were particularly sensitive to betaine. Interestingly, liver betaine concentration was found to be significantly lower in the ZDf fa/fa rats at both 5 and 11 weeks. These results emphasize the importance of betaine metabolism in determining plasma Hcy levels in type 2 diabetes. Diabetes 54: 3245-3251, 2005

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Section: Methodsmentioning

confidence: 99%

Homocysteine Metabolism in ZDF (Type 2) Diabetic Rats

et al. 2005

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“…Methionine synthase was measured using an assay described by Koblin et al [25]. Briefly, liver homogenate (100 μl of 2 mg/ml) was mixed with 100 μl substrate mixture [20 μM cyanocobalamin, 58 mM dithiothreitol (DTT), 0.5 mM SAM, 15 mM DL-homocysteine, 14 mM β-mercaptoethanol, 1 mM methyltetrahydrofolate with 2.5 μCi of 5-[ 14 C]methyl-tetrahydrofolic acid, and 175 mM potassium phosphate buffer, pH 7.5], capped with nitrogen and incubated in the dark at 37°C for 30 min.…”

Section: Methionine Synthasementioning

confidence: 99%

Intrauterine growth restriction leads to changes in sulfur amino acid metabolism, but not global DNA methylation, in Yucatan miniature piglets

MacKay

Brophy

McBreairty

et al. 2012

The Journal of Nutritional Biochemistry

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“…Some anesthetic drugs (propofol, nitrous oxidel and midazolam) have been shown to be toxic to gametes and/or embryos in vitro (5)(6)(7)(8). Toxicity tests on mouse and human oocytes and/or embryos and investigations of follicular fluid and/or serum concentrations have been done (9,10). Of concern is the associations of both gamete and embryotoxicity with different agents (1-3, 10-12).…”

Section: Discussionmentioning

confidence: 99%