Inactivation of Listeria monocytogenes on post-harvest carrot and tomato by gamma radiation, sanitizer, biocontrol treatments and their combinations

Berrios-Rodriguez, Armarynette; Olanya, O. M.; Ukuku, Dike O.; Niemira, Brendan A.; Orellana, Lynette E.; Mukhopadhyay, Sudarsan; Cassidy, Jennifer; Boyd, Glenn

doi:10.1016/j.lwt.2019.108805

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…The potential for the use of biocontrol cultures of lactic acid bacteria (LAB) to reduce pathogen levels has been widely explored for use in food preservation and safety applications ( 38 , 42 , 45 – 49 ). LAB have been also shown to significantly inhibit L. monocytogenes in vitro ( 35 , 38 , 49 – 51 ). For example, Zhao et al ( 52 ) isolated and evaluated two LAB strains for their effectiveness in inhibiting L. monocytogenes in poultry processing facility drains ( 51 – 53 ).…”

Section: Introductionmentioning

confidence: 99%

Ability of Two Strains of Lactic Acid Bacteria To Inhibit Listeria monocytogenes by Spot Inoculation and in an Environmental Microbiome Context

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Ability of Two Strains of Lactic Acid Bacteria To Inhibit Listeria monocytogenes by Spot Inoculation and in an Environmental Microbiome Context

et al. 2022

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“…After the incubation, typical black colonies of S. enterica were counted from replicated and randomized plates. The recovery of pathogen‐injured bacterial cells was determined by counting Salmonella colonies (populations) on the selective medium (XLT‐4) as a proportion of pathogen populations on nonselective (TSA) medium (Berrios‐Rodriguez et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Gamma radiation treatment of postharvest produce for Salmonella enterica reduction on baby carrot and grape tomato

Berrios-Rodriguez

Olanya²,

Niemira³

et al. 2021

Journal of Food Safety

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Occurrences and contamination of Salmonella enterica on produce are a significant food safety issue. The objective of this research was to evaluate the effects of low‐dose gamma radiation on survival and reductions of S. enterica on postharvest carrot and tomato. Irradiation treatments of 0–1 kGy were applied on produce, following pathogen inoculations in replicated experiments. The effects of temperatures (5 and 20°C) and storage times (0, 3, and 7 days) on pathogen reductions were determined. The mean Salmonella populations in untreated control produce varied from 7.12 ± 0.05 to 7.54 ± 0.12 log Cfu/g of produce. Pathogen reductions by gamma radiation were significant (p < .05) and varied with storage times (0–7 days). Salmonella populations were reduced on carrot by 4.3 logs (5°C) and 3.7 logs (20°C) at 1 kGy treatment. On tomato, pathogen reductions at 1 kGy did not differ significantly (p > .05) and were 5.6 and 5.8 logs at 5 and 20°C, respectively. Linear decreases in Salmonella populations occurred as radiation dosage increased. Injury of bacterial cells from radiation treatment was comparatively greater at 1 kGy than other treatments. Low‐dose gamma radiation treatment resulted in significant Salmonella reductions on produce and enhanced postharvest safety of carrot and tomato.

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“…The bacterial stock cultures were maintained in Tryptic Soy broth (TSB; BBL, Sparks, MD, USA) in 20% glycerol, stored at −80 °C. The cultures were revived by transfer of a loopful (~ 0.05 mL) to 9 mL TSB and incubated at 37 °C for L. monocytogenes for 24 h (Olanya et al, 2019;Berrios-Rodriguez et al, 2020a). The bacterial cells were centrifuged (10,000 × g, 5 min, 5 °C) and cell pellets were washed twice with 0.1% BPW (BBL, Sparks, MD, USA).…”

Section: Bacterial Strains and Microbial Propagationmentioning

confidence: 99%

“…L. monocytogenes was quantified on a modified Oxoid agar (MOX) selective agar (BBL, Sparks, MD, USA) by spread-plate method. The media and plating methods were previously used (Berrios-Rodriguez et al, 2020a).…”

Section: Bacteriological Mediamentioning

confidence: 99%

Gamma radiation effects on the survival and reduction of Listeria monocytogenes on carrot and tomato

et al. 2022

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Assessment of pathogen survival is important for food safety. Listeria monocytogenes causes significant produce and food contamination worldwide. The objective of this research was to assess survival and reduction of L. monocytogenes on postharvest carrot and tomato subjected to low-dose radiation at different storage temperatures and times. Radiation levels of 0, 0.25, 0.50, 0.75, and 1 kGy were applied on produce inoculated with L. monocytogenes. Gamma radiation reduced Listeria populations by 5.9 logs on carrot and 3.9 logs on tomato at 3 days of storage (5 °C), while reductions were 4.4 and 4.0 logs on carrot and tomato, respectively; at 7 storage days (5 °C). At 20 °C, Listeria reductions were 2.8 logs on carrot and 4.2 logs on tomato (3 storage days) and pathogen decreases were 2.2 logs on carrot and 2.9 logs on tomato (7 storage days). Although pathogen reductions by radiation treatment varied with storage temperatures and days, the linear decreases were significant as the dosage increased from 0.25 to 1.0 kGy, implying that treatments were efficacious for pathogen inactivation. As low dose gamma radiation (1 kGy) showed substantial reduction of Listeria monocytogenes on fresh carrot and tomato, thus, use of low dose low dose gamma radiation can improve the post-harvest safety of carrot and tomato.

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Inactivation of Listeria monocytogenes on post-harvest carrot and tomato by gamma radiation, sanitizer, biocontrol treatments and their combinations

Cited by 10 publications

References 31 publications

Ability of Two Strains of Lactic Acid Bacteria To Inhibit Listeria monocytogenes by Spot Inoculation and in an Environmental Microbiome Context

Ability of Two Strains of Lactic Acid Bacteria To Inhibit Listeria monocytogenes by Spot Inoculation and in an Environmental Microbiome Context

Gamma radiation treatment of postharvest produce for Salmonella enterica reduction on baby carrot and grape tomato

Gamma radiation effects on the survival and reduction of Listeria monocytogenes on carrot and tomato

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