Inactivation of iron-sulfur cluster biogenesis regulator SufR in Synechocystis sp. PCC 6803 induces unique iron-dependent protein-level responses

Vuorijoki, Linda; Tiwari, Arjun; Kallio, Pauli T.; Aro, Eva‐Mari

doi:10.1016/j.bbagen.2017.02.020

Cited by 18 publications

(10 citation statements)

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“…The dual functions of SufR depend on the redox state of [4Fe-4S] 2+,1+ clusters (Shen et al, 2007). The transcription level of SufR is also regulated by light, oxidative stress, and Fe deficiency (Wang et al, 2004;Seki et al, 2006;Vuorijoki et al, 2017). Specifically, SufR represses the promoter of sufBCDS operon (P1, not P2; two promoters P1 and P2 for sufBCDS operon) under moderate light conditions, and P1 activation results from the derepression by the high light shift (Seki et al, 2006).…”

Section: Suf Mechanismmentioning

confidence: 99%

“…Specifically, SufR represses the promoter of sufBCDS operon (P1, not P2; two promoters P1 and P2 for sufBCDS operon) under moderate light conditions, and P1 activation results from the derepression by the high light shift (Seki et al, 2006). Under the conditions of oxidative stress and Fe deficiency, expression levels of the sufBCDS genes were elevated in sufR (Wang et al, 2004;Vuorijoki et al, 2017). Therefore, sufR is also a transcriptional repressor of the suf operon under Fe-limiting conditions.…”

Section: Suf Mechanismmentioning

confidence: 99%

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Iron–Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria

Feng

2020

Front. Microbiol.

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Iron-sulfur (Fe-S) clusters are ancient and ubiquitous cofactors and are involved in many important biological processes. Unlike the non-photosynthetic bacteria, cyanobacteria have developed the sulfur utilization factor (SUF) mechanism as their main assembly pathway for Fe-S clusters, supplemented by the iron-sulfur cluster and nitrogen-fixing mechanisms. The SUF system consists of cysteine desulfurase SufS, SufE that can enhance SufS activity, SufBC 2 D scaffold complex, carrier protein SufA, and regulatory repressor SufR. The S source for the Fe-S cluster assembly mainly originates from L-cysteine, but the Fe donor remains elusive. This minireview mainly focuses on the biogenesis pathway of the Fe-S clusters in cyanobacteria and its relationship with iron homeostasis. Future challenges of studying Fe-S clusters in cyanobacteria are also discussed.

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Section: Suf Mechanismmentioning

confidence: 99%

Section: Suf Mechanismmentioning

confidence: 99%

Iron–Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria

Feng

2020

Front. Microbiol.

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“…The suf cluster, encoding proteins involved in Fe mobilization and Fe-S cluster assembly, was also downregulated. The expression of both the Fe(II) transporter and the suf cluster is upregulated by Fe deprivation [77][78][79], suggesting a surplus of cellular Fe after low CO 2 treatment. Several neighbouring clusters of genes encoding subunits of metal cation efflux systems such as copper, nickel, zinc, cadmium and cobalt, were upregulated after low CO 2 treatment (Table 4).…”

Section: Low Co 2 Conditions Influence the Expression Of Metal Homeosmentioning

confidence: 99%

Rapid Transcriptional Reprogramming Triggered by Alteration of the Carbon/Nitrogen Balance Has an Impact on Energy Metabolism in Nostoc sp. PCC 7120

Gollan

Muth-Pawlak

Aro

2020

Life

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Nostoc (Anabaena) sp. PCC 7120 is a filamentous cyanobacterial species that fixes N2 to nitrogenous compounds using specialised heterocyst cells. Changes in the intracellular ratio of carbon to nitrogen (C/N balance) is known to trigger major transcriptional reprogramming of the cell, including initiating the differentiation of vegetative cells to heterocysts. Substantial transcriptional analysis has been performed on Nostoc sp. PCC 7120 during N stepdown (low to high C/N), but not during C stepdown (high to low C/N). In the current study, we shifted the metabolic balance of Nostoc sp. PCC 7120 cultures grown at 3% CO2 by introducing them to atmospheric conditions containing 0.04% CO2 for 1 h, after which the changes in gene expression were measured using RNAseq transcriptomics. This analysis revealed strong upregulation of carbon uptake, while nitrogen uptake and metabolism and early stages of heterocyst development were downregulated in response to the shift to low CO2. Furthermore, gene expression changes revealed a decrease in photosynthetic electron transport and increased photoprotection and reactive oxygen metabolism, as well a decrease in iron uptake and metabolism. Differential gene expression was largely attributed to change in the abundances of the metabolites 2-phosphoglycolate and 2-oxoglutarate, which signal a rapid shift from fluent photoassimilation to glycolytic metabolism of carbon after transition to low CO2. This work shows that the C/N balance in Nostoc sp. PCC 7120 rapidly adjusts the metabolic strategy through transcriptional reprogramming, enabling survival in the fluctuating environment.

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“…Targeted proteome analysis using the MRM assay method successfully quantified more proteins than shotgun proteome analysis in Synechocystis sp. PCC 6803 grown in iron-deficient conditions [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Targeted Proteomics of the Central Metabolism and Photosystems in SigE Mutant Strains of Synechocystis sp. PCC 6803

et al. 2018

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A targeted proteome analysis was conducted to investigate the SigE dependent-regulation of central metabolism in Synechocystis sp. PCC 6803 by directly comparing the protein abundance profiles among the wild type, a sigE deletion mutant (ΔsigE), and a sigE over-expression (sigEox) strains. Expression levels of 112 target proteins, including the central metabolism related-enzymes and the subunits of the photosystems, were determined by quantifying the tryptic peptides in the multiple reaction monitoring (MRM) mode of liquid-chromatography–triple quadrupole mass spectrometry (LC–MS/MS). Comparison with gene-expression data showed that although the abundance of Gnd protein was closely correlated with that of gnd mRNA, there were poor correlations for GdhA/gdhA and glycogen degradation-related genes such as GlgX/glgX and GlgP/glgP pairs. These results suggested that the regulation of protein translation and degradation played a role in regulating protein abundance. The protein abundance profile suggested that SigE overexpression reduced the proteins involved in photosynthesis and increased GdhA abundance, which is involved in the nitrogen assimilation pathway using NADPH. The results obtained in this study successfully demonstrated that targeted proteome analysis enables direct comparison of the abundance of central metabolism- and photosystem-related proteins.

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Inactivation of iron-sulfur cluster biogenesis regulator SufR in Synechocystis sp. PCC 6803 induces unique iron-dependent protein-level responses

Abstract: The study provides new insight into the regulation of Fe-S cluster biogenesis via suf operon, and the associated wide-ranging protein-level changes in photosynthetic cyanobacteria.

Cited by 18 publications

References 64 publications

Iron–Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria

Iron–Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria

Rapid Transcriptional Reprogramming Triggered by Alteration of the Carbon/Nitrogen Balance Has an Impact on Energy Metabolism in Nostoc sp. PCC 7120

Comparative Targeted Proteomics of the Central Metabolism and Photosystems in SigE Mutant Strains of Synechocystis sp. PCC 6803

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