Inactivation of <i>Pseudomonas aeruginosa</i> biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B

Argyraki, Aikaterini; Markvart, Merete; Bjørndal, Lars; Bjarnsholt, Thomas; Petersen, Paul Michael

doi:10.1117/1.jbo.22.6.065004

Cited by 16 publications

(20 citation statements)

References 31 publications

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“…has reported disinfection potential of catheter lumens by UVC LEDs 5 . Recently, we have observed that UVB irradiation is more efficient than UVC, in eradicating biofilms plated on cellulose nitrate membrane filters 6 . However, the ultraviolet (UV) wavelength dependent eradication efficiency for Pseudomonas aeruginosa biofilms has not been reported.…”

Section: Introductionmentioning

confidence: 99%

UV light assisted antibiotics for eradication of in vitro biofilms

Argyraki

Markvart

Stavnsbjerg

et al. 2018

Sci Rep

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The overuse of antibiotics is accelerating the bacterial resistance, and therefore there is a need to reduce the amount of antibiotics used for treatment. Here, we demonstrate in vitro that specific wavelengths in a narrow range around 296 nm are able to eradicate bacteria in the biofilm state (grown for 24 hours) more effectively, than antibiotics and the combination of irradiation and antibiotics is even better, introducing a novel concept light assisted antibiotics. The investigated wavelength range was 249 nm to 338 nm with an approximate step of 5 nm. The novel concept that consists of a UV irradiation treatment followed by a tobramycin treatment can significantly reduce the amount of antibiotics needed for eradicating mature bacterial biofilms. The efficiency of the proposed light assisted antibiotics method was compared to combinatory antibiotic treatment and highly concentrated antibiotic monotherapy. The eradication efficacies, on mature biofilms, achieved by light assisted antibiotic and by the antibiotic monotherapy at approximately 10-fold higher concentration, were equivalent. The present achievement could motivate the development of light assisted antibiotic treatments for treating infections.

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Section: Introductionmentioning

confidence: 99%

UV light assisted antibiotics for eradication of in vitro biofilms

Argyraki

Markvart

Stavnsbjerg

et al. 2018

Sci Rep

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“…In the biofilm state, the smaller penetration achieved by shorter wavelengths is expected to reduce the eradication efficacy of UVC [6]. In the UVA region and longer wavelengths bacterial eradication is dictated only by indirect pathways like generation of reactive oxygen species, and therefore, the eradication efficiency is much lower [43].…”

Section: Discussionmentioning

confidence: 99%

“…The bacterial strain used at the experiments was P. aeruginosa PAO1 [52]. A detailed description about the biofilm preparation can be found in Argyraki et al [6]. The micropore assay methodology used to form the biofilms can be found in Bjarnsholt et al [53].…”

Section: Methodsmentioning

confidence: 99%

“…The method for CFU determination can be found in Argyraki et al [6]. The serial dilutions in the cases of UVB irradiation treatment versus topically administrated antibiotics and light assisted antibiotics were performed from 10 0 (no dilution) to 10 −7 and the spotted volume was 10 μL; resulting in a detection limit of 100 bacteria per ml.…”

Section: Methodsmentioning

confidence: 99%

“…Bak et al has reported in vivo disinfection potential of catheter lumens by UVC LEDs [5]. Recently, we have observed that UVB irradiation is more efficient than UVC, in eradicating biofilms plated on cellulose nitrate membrane filters [6]. However, the ultraviolet (UV) wavelength dependent eradication efficiency for Pseudomonas aeruginosa biofilms has not been reported.…”

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confidence: 99%

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Light assisted antibiotics

Argyraki

Markvart

Stavnsbjerg

et al. 2017

Preprint

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The overuse of antibiotics is accelerating the bacterial resistance, and therefore there is a need to reduce the amount of antibiotics used for treatment. Here, we demonstrate that specific wavelengths in a narrow range around 296 nm are able to eradicate bacteria in the biofilm state more effectively, than antibiotics and the combination of irradiation and antibiotics is even better, introducing a novel concept light assisted antibiotics. The investigated wavelength range was 249 nm to 338 nm with an approximate step of 5 nm. The novel concept can significantly reduce the amount of antibiotics needed for eradicating mature bacterial biofilms. The irradiation treatment was combined with tobramycin and its efficiency was compared to combinatory antibiotic treatment and highly concentrated antibiotic monotherapy. The eradication efficacies, on mature biofilms, achieved by light assisted antibiotic and by the antibiotic monotherapy at 10-fold higher concentration, were equivalent. The present achievement could motivate the development of light assisted antibiotic treatments for treating infections.There is a worldwide increasing awareness and concern about the 1 antibiotic resistance, which in the future could prevent effective

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Cell density and extracellular matrix composition mitigate bacterial biofilm sensitivity to UV-C LED irradiation

Labadie,

Marchal,

Merbahi

et al. 2024

Appl Microbiol Biotechnol

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Ultraviolet-C light-emitting diodes (UV-C LEDs) are an emerging technology for decontamination applications in different sectors. In this study, the inactivation of bacterial biofilms was investigated by applying an UV-C LED emitting at 280 nm and by measuring both the influence of the initial cell density (load) and presence of an extracellular matrix (biofilm). Two bacterial strains exposing diverging matrix structures and biochemical compositions were used: Pseudomonas aeruginosa and Leuconostoc citreum. UV-C LED irradiation was applied at three UV doses (171 to 684 mJ/cm2) on both surface-spread cells and on 24-h biofilms and under controlled cell loads, and bacterial survival was determined. All surface-spread bacteria, between 105 and 109 CFU/cm2, and biofilms at 108 CFU/cm2 showed that bacterial response to irradiation was dose-dependent. The treatment efficacy decreased significantly for L. citreum surface-spread cells when the initial cell load was high, while no load effect was observed for P. aeruginosa. Inactivation was also reduced when bacteria were grown under a biofilm form, especially for P. aeruginosa: a protective effect could be attributed to abundant extracellular DNA and proteins in the matrix of P. aeruginosa biofilms, as revealed by Confocal Laser Scanning Microscopy observations. This study showed that initial cell load and exopolymeric substances are major factors influencing UV-C LED antibiofilm treatment efficacy. Key points • Bacterial cell load (CFU/cm2) could impact UV-C LED irradiation efficiency • Characteristics of the biofilm matrix have a paramount importance on inactivation • The dose to be applied can be predicted based on biofilm properties Graphical Abstract

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Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B

Cited by 16 publications

References 31 publications

UV light assisted antibiotics for eradication of in vitro biofilms

UV light assisted antibiotics for eradication of in vitro biofilms

Light assisted antibiotics

Cell density and extracellular matrix composition mitigate bacterial biofilm sensitivity to UV-C LED irradiation

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