Inactivation of foot-and-mouth disease virus A/IRN/8/2015 with commercially available lysis buffers

Wood, Britta A.; Mioulet, Valérie; Henry, Elisabeth; Gray, Ashley R.; Azhar, Mehreen; Thapa, Barsha; Diederich, Sandra; Hoffmann, Bernd; King, Donald P.; Eschbaumer, Michael

doi:10.1016/j.jviromet.2020.113835

Cited by 13 publications

(13 citation statements)

References 18 publications

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“…The ability of AVL to inactivate pathogenic viruses was debated (8–10) but there is no data for ATL and VXL. A total of ten different protocols using AVL, ATL and VXL alone or in association with ethanol or Triton-X100 were studied on SARS-CoV-2 according to the French version of the European recommended procedure (NF EN 14476+A2) (16), as previously shown for other viruses such as Ebola virus or Foot and Mouth Disease virus (8,10,22,23). Our results are in line with data reported for Zaire Ebolavirus (10).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of heating and chemical protocols for inactivating SARS-CoV-2

Pastorino

Touret

Gilles

et al. 2020

Preprint

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Clinical samples collected in COVID-19 patients are commonly manipulated in BSL-2 laboratories for diagnostic purpose. We used the French norm NF-EN-14476+A2 derived from the European standard EN-14885. To avoid the risk of exposure of laboratory workers, we showed that Triton-X100 must be added to guanidinium thiocyanate-lysis buffers to obtain a 6-log reduction of infectious virus. Although heating protocol consisting of 92°C-15min was more effective rather than 56°C-30min and 60°C-60min to achieve 6-log reduction, it is not amenable for molecular detection on respiratory specimens because of important decrease of detectable RNA copies in the treated sample vs untreated sample. The 56°C-30min and 60°C-60min should be used for inactivation of serum / plasma samples for serology because of the 5log10 reduction of infectivity and low viral loads in blood specimens.

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Section: Discussionmentioning

confidence: 99%

Evaluation of heating and chemical protocols for inactivating SARS-CoV-2

Pastorino

Touret

Gilles

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The ability of AVL to inactivate pathogenic viruses was debated, but there are no data for ATL and VXL. A total of ten different protocols using AVL, ATL, and VXL alone, or in association with ethanol or Triton-X100 were studied on SARS-CoV-2 according to the French version of the European recommended procedure (NF EN 14476+A2) [ 15 ], as previously shown for other viruses such as Ebola virus or foot and mouth disease virus [ 11 , 13 , 17 , 18 ]. Our results are in line with data reported for the Zaire ebolavirus [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Pastorino

Touret

Gilles

et al. 2020

Viruses

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Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 min contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.

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“…Several chemical and physical methods of viral inactivation have been evaluated for different pathogens, as a way to provide greater safety for professionals involved in the handling of potentially infectious samples and lower costs with laboratory infrastructure [10][11][12][13][14][15][16]. Among the chemical methods evaluated, the most commonly used products contain a chaotropic salt (guanidine), which acts as a surfactant/ denaturant agent culminating in virus inactivation [12].…”

Section: Introductionmentioning

confidence: 99%

The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel

et al. 2021

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Accurate testing to detect SARS-CoV-2 RNA is key to counteract the virus spread. Nonetheless, the number of diagnostic laboratories able to perform qPCR tests is limited, particularly in developing countries. We describe the use of a virusinactivating, denaturing solution (DS) to decrease virus infectivity in clinical specimens without affecting RNA integrity. Swab samples were collected from infected patients and from laboratory personnel using a commercially available viral transport solution and the in-house DS. Samples were tested by RT-qPCR, and exposure to infective viruses was also accessed by ELISA. The DS used did not interfere with viral genome detection and was able to maintain RNA integrity for up to 16 days at room temperature. Furthermore, virus loaded onto DS were inactivated, as attested by attempts to grow SARS-CoV-2 in cell monolayers after DS desalt filtration to remove toxic residues. The DS described here provides a strategy to maintain diagnostic accuracy and protects diagnostic laboratory personnel from accidental infection, as it has helped to protect our lab crew.

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Inactivation of foot-and-mouth disease virus A/IRN/8/2015 with commercially available lysis buffers

Cited by 13 publications

References 18 publications

Evaluation of heating and chemical protocols for inactivating SARS-CoV-2

Evaluation of heating and chemical protocols for inactivating SARS-CoV-2

Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

The use of denaturing solution as collection and transport media to improve SARS-CoV-2 RNA detection and reduce infection of laboratory personnel

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