Inactivation of food-borne enteropathogenic bacteria and spoilage fungi using pulsed-light

Anderson, John G.; Rowan, Neil J.; MacGregor, S.J.; Fouracre, R.A.; Farish, O.

doi:10.1109/27.842870

Cited by 178 publications

(127 citation statements)

References 8 publications

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“…This may be explained by the increase of beach users in the third sampling period as well as by the higher resistance of fungi to solar exposure (Anderson et al, 2000). In fact, fungal spores can survive between 25 and 360 days in the environment whereas the survival of enteric bacteria on the surface of dry sand may be less.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic fungi: An unacknowledged risk at coastal resorts? New insights on microbiological sand quality in Portugal

Sabino

Veríssimo

Cunha

et al. 2011

Marine Pollution Bulletin

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Whilst the potential impact on beach users from microorganisms in water has received considerable attention, there has been relatively little investigation into microbial contaminants in sand. 33 beaches across Portugal were analyzed during a five year period (2006)(2007)(2008)(2009)(2010) to determine the presence of yeasts, pathogenic fungi, dermatophytes, total coliforms, E. coli and intestinal enterococci in sand.Our results showed that 60.4% of the samples were positive for fungi and that 25.2% were positive for the bacterial parameters. The most frequent fungal species found were Candida sp.and Aspergillus sp., whereas intestinal enterococci were the most frequently isolated bacteria.Positive associations were detected among analyzed parameters and country-regions but none among those parameters and sampling period.Regarding threshold values, we propose 15 cfu/g for yeasts, 17 cfu/g for potential pathogenic fungi, 8 cfu/g for dermatophytes. 84 cfu/g for coliforms, 250 cfu/g for E. coli, and 100 cfu/g for intestinal enterococciHighlights:x 60.4% of the analyzed marine beach sand samples were positive for the studied fungi x Candida sp. and Aspergillus sp. were the most frequent fungal species found x 25.2% of the analyzed marine beach sand samples were positive to the studied bacteria

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Section: Discussionmentioning

confidence: 99%

Pathogenic fungi: An unacknowledged risk at coastal resorts? New insights on microbiological sand quality in Portugal

Sabino

Veríssimo

Cunha

et al. 2011

Marine Pollution Bulletin

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“…Marquenie et al [10] also reported a maximal inactivation of 3 and 4 log10 units for conidia of Botrytis cinerea and Monilinia fructigena in vitro after pulse UV treatment of 120 s at the fluence of 0.10 J cm -2 . The treated fungal populations of Aspergillus niger and Fusarium culmorum were reduced by 3 to 4.5 log10 orders after 1,000 light pulses of the 3 J UV intensity light [26]. The maximal log10 reduction was close to 1 with a pulsed UV-light fluence of 1.2 J cm -2 for Aspergillus niger inoculated in sugar syrup, whereas on agar, the log10 reduction for the same fungi was even lower than 1 for the same fluence [39,40].…”

Section: Pulsed Uv Light and Microbiological Analysismentioning

confidence: 84%

“…It has been used for centuries as a leavening agent for bread and as a fermenter of alcoholic beverages. With its prolonged use in industrial applications, this yeast has also been the model for various studies on the principles of microbiology [26]. The presence of S. cerevisiae in the product may be due to contamination of the bakery environment with commercial baker's yeast.…”

Section: Fungal Diversitymentioning

confidence: 99%

Analysis of Fungal Diversity in Ready-to-Eat Pizza and Effectiveness of Pulsed Ultraviolet-Light Treatment for Inactivation of Mold on Agar Surface

Ngadi¹

2015

J Bioprocess Biotech

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Fungal contamination is a significant issue in the food production industry; therefore, identification and characterization of food spoilage fungi would allow for early intervention to limit the amount of fungal contamination, particularly in cereal-based industries. In the present study, culture-dependent and culture-independent methods were applied to study the microbiota of ready-to-eat pizza. The study was pursued by evaluating the effectiveness of a broad-spectrum pulsed ultraviolet light for the decontamination of Penicillium roqueforti (a dominant spoilage mold in bakery products) on the surface of solid agar as a representative of a flat food surface. The average population of mesophilic aerobic bacteria (MAB), mesophilic anaerobic bacteria (MANB), lactic acid bacteria (LAB), molds and yeasts (M+Y) on naturally spoiled pre-cooked pizza were 6.7 ± 0.5, less than 2.3, 2.8 ± 0.6 and 5.4 ± 0.4 log10 CFU g -1 , respectively. Cloning and sequencing identified at least 5 genera and species of fungi (Penicillium spp., Saccharomyces spp., Rhodotorula mucilaginosa, Monascus fuliginosus, Galactomyces geotrichum) from 2 phyla (Ascomycota and Basidiomycota). Pulsed light process parameters evaluated were treatment time (1, 3, 5, 7 and 10 min) and voltage input (500, 750 and 1,000 V) at 5 cm distance from the pulsed UV-light. An increase of the input voltage of the lamps and of the duration of the treatment resulted in a higher inactivation of P. roqueforti. The population of P. roqueforti was reduced after 10 min of exposure to pulsed light by 3.74, 5.36 and 6.14 log10 CFU ml -1 at 500, 750 and 1,000 V, respectively. The inactivation kinetics was best described by the Weibull model (2 parameters) with the smallest root mean squared error (RMSE) and R 2 ≥ 0.92. The results of this work show that pulsed light is a promising technique for fungi elimination or decontamination in the bakery industry.

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“…Recent work has focused on the use of pulsed, polychromatic xenon flashlamps for microbial inactivation, as this technology enables rapid energy delivery and wide antimicrobial activity, whilst being more environmentally friendly than CW-UV lamps as they do not contain mercury [48][49][50]. Using this technology, Xenex has developed fullyautomated robotic decontamination systems for clinical room decontamination applications, and have been marketed as "Germ Zapping Robots"™.…”

Section: Pulsed Uv-light For Whole-room Decontaminationmentioning

confidence: 99%

Decontamination of the Hospital Environment: New Technologies for Infection Control

Maclean

McKenzie

Moorhead

et al. 2015

Curr Treat Options Infect Dis

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Inactivation of food-borne enteropathogenic bacteria and spoilage fungi using pulsed-light

Cited by 178 publications

References 8 publications

Pathogenic fungi: An unacknowledged risk at coastal resorts? New insights on microbiological sand quality in Portugal

Pathogenic fungi: An unacknowledged risk at coastal resorts? New insights on microbiological sand quality in Portugal

Analysis of Fungal Diversity in Ready-to-Eat Pizza and Effectiveness of Pulsed Ultraviolet-Light Treatment for Inactivation of Mold on Agar Surface

Decontamination of the Hospital Environment: New Technologies for Infection Control

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