Inactivation of Escherichia coli and Staphylococcus aureus by Ultrasound

Sesal, Nüzhet Cenk; Kekeç, Özge

doi:10.7863/ultra.33.9.1663

Cited by 19 publications

(15 citation statements)

References 17 publications

(24 reference statements)

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“…Ultrasonication of the mixture was then carried out by a sonicator (SK3310HP, KUDOS) at 53 kHz, 180 W and 30 °C for 20 min. (Step 4) to disrupt cell membranes and release cellular contents, such as hemoglobin from the red blood cell (RBC)—so called sonoporation 36 , 37 —more thoroughly, while avoiding bacteria inactivation 38 – 40 . After the resultant sample tube underwent the same centrifugation procedure (Step 5), the supernatant—containing hemoglobin, cellular fragments and other low-density components, etc .—was replaced with sterile water (Step 6).…”

Section: Methodsmentioning

confidence: 99%

Rapid antibiotic susceptibility testing of bacteria from patients’ blood via assaying bacterial metabolic response with surface-enhanced Raman spectroscopy

Han

Lin

Cheng

et al. 2020

Sci Rep

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Blood stream infection is one of the major public health issues characterized with high cost and high mortality. Timely effective antibiotics usage to control infection is crucial for patients' survival. The standard microbiological diagnosis of infection however can last days. the delay in accurate antibiotic therapy would lead to not only poor clinical outcomes, but also to a rise in antibiotic resistance due to widespread use of empirical broad-spectrum antibiotics. An important measure to tackle this problem is fast determination of bacterial antibiotic susceptibility to optimize antibiotic treatment. We show that a protocol based on surface-enhanced Raman spectroscopy can obtain consistent antibiotic susceptibility test results from clinical blood-culture samples within four hours. the characteristic spectral signatures of the obtained spectra of Staphylococcus aureus and Escherichia coli-prototypic Gram-positive and Gram-negative bacteria-became prominent after an effective pretreatment procedure removed strong interferences from blood constituents. Using them as the biomarkers of bacterial metabolic responses to antibiotics, the protocol reported the susceptibility profiles of tested drugs against these two bacteria acquired from patients' blood with high specificity, sensitivity and speed. Blood stream infection (BSI)-defined as the presence of viable bacteria in blood (i.e., bacteremia) documented by a positive blood culture result 1-arouses morbidity and mortality worldwide 2. It is the primary cause of sepsis, which is a major public health concern with particular high medical cost 3,4. Although the number of BSI incidence bears demographic difference, it shows increasing trend over time 5,6 and therefore remains an urgent medical challenge. Timely administration of appropriate antibiotic therapy is critical to avoiding its progression. A retrospective analysis study based on a large dataset showed that there was a linear increase in the risk of mortality for each hour delay in antibiotic administration 7 , which was confirmed by a recent study 8. However, owing to lack of timely microbiological evidence, antibiotics are usually forced to start empirically, rather than precisely upon specific target(s) 9. Improper antibiotic selection was not uncommon and the mortality markedly increased 10. Moreover, the initial empirical use of broad-spectrum antibiotics 11 , although prudent, inevitably

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Section: Methodsmentioning

confidence: 99%

Rapid antibiotic susceptibility testing of bacteria from patients’ blood via assaying bacterial metabolic response with surface-enhanced Raman spectroscopy

Han

Lin

Cheng

et al. 2020

Sci Rep

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“…Unorthodox combination of low frequency ultrasound (LFU) and antimicrobial agents may bridge the gap in current treatment against biofilm infections. As a noninvasive physical method, ultrasonic irradiation can be achieved under ambient conditions of pressure and temperature without any chemical compounds, making it one of the most attractive methods [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Multiple Low Frequency Ultrasound Enhances Bactericidal Activity of Vancomycin against Methicillin-ResistantStaphylococcus aureusBiofilms

Wang

Wen

Liu

et al. 2018

BioMed Research International

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Methicillin-resistant Staphylococcus aureus (MRSA) biofilm infections are difficult to treat due to the high antimicrobial resistance of biofilm. Therefore, new treatments are needed for more effective bacteria clearance. This study was to investigate whether low frequency ultrasound (LFU) can enhance the activity of antimicrobial agents against MRSA biofilm infection. Broth microdilution method was used to determine the minimum inhibitory concentration (MIC) of vancomycin (VAN), linezolid (LIN), and levofloxacin (LEV) against three clinical isolated strains, including one methicillin-susceptible Staphylococcus aureus (MSSA) strain and two MRSA strains. Effects of various influencing factors, such as antimicrobial agents, drug concentrations, ultrasonic intensity, and single (S-LFU, 5 or 15 min) or multiple ultrasound (M-LFU, 5 min every 8 h), on the inhibition of biofilms were investigated. The bactericidal effects of S-LFU or M-LFU on MRSA or MSSA biofilms were determined by colony counts. Right after ultrasound, synergistic effects were observed in groups of S-LFU combined with three antimicrobial agents against MSSA biofilm, but for MRSA biofilm, only S-LFU plus VAN had synergistic effect. At the time point of 24 h, M-LFU plus VAN treatment had synergistic bactericidal effect against MRSA and MSSA biofilms, and the synergy showed that VAN is concentration-dependent, but no synergistic effects were observed in all S-LFU combination groups. In conclusion, combination of M-LFU and antimicrobial agents had a better synergistic effect than S-LFU against MRSA or MSSA biofilm. LFU may be useful in treating biofilm infection in the future.

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“…The strongest treatment reduced all bacteria significantly but S. aureus was reduced only 0.55 log. Similarly, Sesal and Kekeç (2014) found that ultrasonic irradiation (30 kHz frequency and power of 100 W) is not a suitable method for the elimination of S. aureus in physiological water samples.…”

Section: Resultsmentioning

confidence: 99%

Physicochemical characteristics and shelf life of beef treated with high‐intensity ultrasound

Valenzuela

García-Galicia

Paniwnyk

et al. 2021

J. Food Process. Preserv.

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This study explored the impact of high‐intensity ultrasound (US) (HIU; 40 kHz, 11 W/cm2) on physicochemical characteristics and shelf life of Semitendinosus beef muscle during storage for 0, 3, 6, and 9 d at 4°C. HIU decreased the pH and the global difference of color along with the storage (from 5.99 to 1.43 ΔΕ) in comparison with not sonicated beef. The drip loss of beef decreased with US and with storage, but it was similar to the control at the end of 9 d of storage. No difference in water‐holding capacity and SF of sonicated beef was observed compared to the controls. Counts of mesophilic, psychrophilic, Staphylococcus spp., and coliform bacteria decreased with HIU application. HIU has proven to be a technology that could be efficiently used to increase the shelf life of beef retaining its technological quality. Practical applications In meat processing, sonication can serve as an alternative processing technique to the traditional methods of meat preservation and is considered a green, versatile, and emerging technology. Ultrasonication was remarkably effective for inactivation of beef microbiota showing a growing effect with storage time. US has minimal effects on physicochemical properties of fresh and stored beef. Hence, these findings offer an opportunity for the meat industry to increase the shelf life of beef retaining its technological quality.

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Inactivation of Escherichia coli and Staphylococcus aureus by Ultrasound

Cited by 19 publications

References 17 publications

Rapid antibiotic susceptibility testing of bacteria from patients’ blood via assaying bacterial metabolic response with surface-enhanced Raman spectroscopy

Rapid antibiotic susceptibility testing of bacteria from patients’ blood via assaying bacterial metabolic response with surface-enhanced Raman spectroscopy

Multiple Low Frequency Ultrasound Enhances Bactericidal Activity of Vancomycin against Methicillin-ResistantStaphylococcus aureusBiofilms

Physicochemical characteristics and shelf life of beef treated with high‐intensity ultrasound

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