Inactivation of enzymes involved in nitrogen assimilation in Pseudomonas aeruginosa

Smits, Rob; Drift, C. van der

doi:10.1007/bf00404942

Cited by 10 publications

(11 citation statements)

References 3 publications

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“…In addition, two alkane hydroxylases encoded by alkMa and alkMb are induced by n-alkanes in different ways, that is, the former is induced by long chain alkanes (> C 22 ) in solid form and the latter is preferentially induced by liquid alkanes (C 16 to C 22 ; Tani et al, 2001). In P. aeruginosa strains, alkane hydroxylases that are responsible for the degradation of alkanes of different lengths (C 6 -C 11 or C 12 -C 16 ) have been identified (Smits et al, 2003). However, P. aeruginosa strains may have a third type of alkane hydroxylase specific to very long chain hydrocarbons such as C 36 or C 40 .…”

Section: Degradation Of Individual N-alkanesmentioning

confidence: 99%

Degradation of long-chain n-alkanes (C36 and C40) by Pseudomonas aeruginosa strain WatG

Hasanuzzaman¹,

Ueno

Ichikawa

et al. 2007

International Biodeterioration & Biodegradation

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Pseudomonas aeruginosa strain WatG was unable to utilize either n-hexatriacontane (C 36 ) or n-tetracontane (C 40 ), which are both insoluble in a mineral salts medium (MSM), as sole carbon source. However, when C 36 and C 40 were added to MSM containing crude oil, more than 25% of each of the compounds was degraded by strain WatG after two weeks at 30°C. These results demonstrate that strain WatG has the ability to degrade long chain alkanes up to C 40 , when they are solubilized by crude oil components.

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Section: Degradation Of Individual N-alkanesmentioning

confidence: 99%

Degradation of long-chain n-alkanes (C36 and C40) by Pseudomonas aeruginosa strain WatG

Hasanuzzaman¹,

Ueno

Ichikawa

et al. 2007

International Biodeterioration & Biodegradation

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“…Although alkane chemotaxis has been observed in bacterial isolates of Flavimonas oryzihabitans 30 and P. aeruginosa PAO1 (ref. 31), the underlying machinery has not been identified 32 . In addition, alkane uptake is an essential step before utilization, but the mechanism by which uptake occurs is also unknown.…”

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confidence: 99%

The long-chain alkane metabolism network of Alcanivorax dieselolei

2014

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Alkane-degrading bacteria are ubiquitous in marine environments, but little is known about how alkane degradation is regulated. Here we investigate alkane sensing, chemotaxis, signal transduction, uptake and pathway regulation in Alcanivorax dieselolei. The outer membrane protein OmpS detects the presence of alkanes and triggers the expression of an alkane chemotaxis complex. The coupling protein CheW2 of the chemotaxis complex, which is induced only by long-chain (LC) alkanes, sends signals to trigger the expression of Cyo, which participates in modulating the expression of the negative regulator protein AlmR. This change in turn leads to the expression of ompT1 and almA, which drive the selective uptake and hydroxylation of LC alkanes, respectively. AlmA is confirmed as a hydroxylase of LC alkanes. Additional factors responsible for the metabolism of medium-chain-length alkanes are also identified, including CheW1, OmpT1 and OmpT2. These results provide new insights into alkane metabolism pathways from alkane sensing to degradation.

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“…Transcription of both genes is activated by QscR (16). PA1897 codes for a polypeptide of unknown function, and PA5351 codes for rubredoxin 1, an electron carrier protein that functions in an alkane hydroxylase system (17,32,33,38). Direct binding to PA1897 and PA5351 was established by electrophoretic mobility shift assays (EMSAs) with purified His-tagged QscR (15).…”

mentioning

confidence: 99%

Acyl-Homoserine Lactone Binding to and Stability of the Orphan Pseudomonas aeruginosa Quorum-Sensing Signal Receptor QscR

Oinuma

Greenberg

2011

J Bacteriol

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The Pseudomonas aeruginosa transcription factor QscR responds to a variety of fatty acyl-homoserine lactones (HSLs), including N-3-oxododecanoyl-HSL (3OC12-HSL), which is produced and detected by the P. aeruginosa quorum-sensing circuit LasI and LasR. As is true for LasR and many other acyl-HSL-dependent transcription factors, production of soluble QscR in sufficient amounts for purification requires growth of recombinant bacteria in the presence of an appropriate acyl-HSL. QscR is thought to bind 3OC12-HSL relatively weakly compared to LasR, and unlike LasR, binding of purified QscR to target DNA was shown to strongly depend on exogenously added 3OC12-HSL. We show that purified QscR is dimeric at sufficiently high concentrations and monomeric at lower concentrations. Furthermore, QscR bound 3OC12-HSL more tightly than previously believed. Purified QscR retained 3OC12-HSL, and at sufficiently high concentrations, it bound target DNA in the absence of added 3OC12-HSL. We also obtained soluble QscR from recombinant Escherichia coli grown in the presence of N-3-oxohexanoyl-HSL (3OC6-HSL) instead of 3OC12-HSL, and because 3OC6-HSL bound much more loosely to QscR than other acyl-HSLs tested, we were able to exchange 3OC6-HSL with other acyl-HSLs in vitro and then estimate binding affinities of QscR for different acyl-HSLs and for target DNA. Our data support a model whereby QscR polypeptides fold properly in the absence of an acyl-HSL, but soluble, acyl-HSL-free QscR does not accumulate because it is subject to rapid aggregation or proteolysis.

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Inactivation of enzymes involved in nitrogen assimilation in Pseudomonas aeruginosa

Cited by 10 publications

References 3 publications

Degradation of long-chain n-alkanes (C36 and C40) by Pseudomonas aeruginosa strain WatG

Degradation of long-chain n-alkanes (C36 and C40) by Pseudomonas aeruginosa strain WatG

The long-chain alkane metabolism network of Alcanivorax dieselolei

Acyl-Homoserine Lactone Binding to and Stability of the Orphan Pseudomonas aeruginosa Quorum-Sensing Signal Receptor QscR

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