Inactivation of Ebola Virus with 60CO Irradiation

Lupton, H W

doi:10.1093/infdis/143.2.291

Cited by 20 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously reported that γ-Flu preparations can induce cross-reactive Tc cell responses [5]. Gamma-irradiation is the preferred method of inactivation of highly infectious agents for biochemical analysis, including Ebola, Marburg and Lassa viruses [6], [7], [8]. It inactivates virus infectivity by generating strand-breaks in the genetic material and has the further advantage, compared with chemical agents, of high penetration into and through biological materials [7].…”

Section: Introductionmentioning

confidence: 99%

Intranasal Flu Vaccine Protective against Seasonal and H5N1 Avian Influenza Infections

et al. 2009

View full text Add to dashboard Cite

BackgroundInfluenza A (flu) virus causes significant morbidity and mortality worldwide, and current vaccines require annual updating to protect against the rapidly arising antigenic variations due to antigenic shift and drift. In fact, current subunit or split flu vaccines rely exclusively on antibody responses for protection and do not induce cytotoxic T (Tc) cell responses, which are broadly cross-reactive between virus strains. We have previously reported that γ-ray inactivated flu virus can induce cross-reactive Tc cell responses.Methodology/Principal FindingHere, we report that intranasal administration of purified γ-ray inactivated human influenza A virus preparations (γ-Flu) effectively induces heterotypic and cross-protective immunity. A single intranasal administration of γ-A/PR8[H1N1] protects mice against lethal H5N1 and other heterotypic infections.Conclusions/SignificanceIntranasal γ-Flu represents a unique approach for a cross-protective vaccine against both seasonal as well as possible future pandemic influenza A virus infections.

show abstract

Section: Introductionmentioning

confidence: 99%

Intranasal Flu Vaccine Protective against Seasonal and H5N1 Avian Influenza Infections

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Immunological studies of infected humans or animal models of infections with the agents of VHF have been limited by the requirement of BSL4 laboratories because of safety considerations. A number of methods have been used successfully to inactivate RNA and DNA viruses, including treatment with ␥-irradiation [Lupton, 1981;Mitchell and McCormick, 1984], UV light [Chepurnov et al, 1995], ␤-propiolactone [Budowsky and Zalesskaya, 1991], paraformaldehyde (PFA), sodium dodecyl sulfate (SDS), and heat denaturation [Mitchell and McCormick, 1984]. Most of these techniques have been demonstrated to inactivate viruses effectively and not to affect a number of blood and serum components [Elliott et al, 1982;Mitchell and McCormick, 1984] and nonviral protein antigens [Loutfy et al, 1998].…”

Section: Introductionmentioning

confidence: 99%

Cytokine measurement in biological samples after physicochemical treatment for inactivation of biosafety level 4 viral agents

1999

View full text Add to dashboard Cite

Physicochemical techniques such as gamma-irradiation, membrane disruption by detergents like sodium dodecyl sulfate (SDS), and fixation with formaldehyde or paraformaldehyde are routinely used to inactivate biological specimens from patients and animals infected with Filoviruses and Arena viruses that must be studied in BSL 4 facilities. The effects of these inactivation techniques on the levels of immunologically active proteins like cytokines and chemokines are not known. Therefore, we investigated the effect of several decontamination techniques on the immunoreactivity and bioactivity of the inflammatory cytokines IL-1beta, IL-6, TNF-alpha, IL-12, and IFN-gamma, the anti-inflammatory cytokine IL-10, and the chemokine IL-8 in biological specimens. SDS (96%-100% reduction), paraformaldehyde treatment (11%-100% reduction), and heat denaturation (75%-100% reduction) were found to decrease markedly the levels of all cytokines and chemokines as measured by enzyme-linked immunosorbent assays. In contrast, gamma-irradiation was found to have little or no effect on the immunoreactivity of these cytokines/chemokines and on the biological activity of tumor necrosis factor (TNF) alpha. Our data suggest that, of the agents tested, gamma-irradiation is the preferred technique for inactivation of biological specimens containing viral agents that require the use of BSL 4 for immunological studies.

show abstract

“…Isolate E718 (known as 718 [ 5 ], E718 [ 6 – 14 ), E-718 [ 15 – 17 ], and E 718 [ 6 , 15 , 18 – 21 ]) is one of the EBOV isolates found in 1976. The origin of E718 is usually not mentioned, or it is stated only that E718 was derived from an acute-phase human blood sample ( 5 , 10 – 14 , 16 , 17 , 20 , 21 ). Other authors elaborate that the isolate was obtained by S. R. Pattyn (Instituut voor Tropische Geneeskunde [ITG], Antwerp, Belgium) ( 6 , 15 , 18 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Reidentification of Ebola Virus E718 and ME as Ebola Virus/H.sapiens-tc/COD/1976/Yambuku-Ecran

et al. 2014

View full text Add to dashboard Cite

show abstract

Inactivation of Ebola Virus with 60CO Irradiation

Cited by 20 publications

References 0 publications

Intranasal Flu Vaccine Protective against Seasonal and H5N1 Avian Influenza Infections

Intranasal Flu Vaccine Protective against Seasonal and H5N1 Avian Influenza Infections

Cytokine measurement in biological samples after physicochemical treatment for inactivation of biosafety level 4 viral agents

Reidentification of Ebola Virus E718 and ME as Ebola Virus/H.sapiens-tc/COD/1976/Yambuku-Ecran

Contact Info

Product

Resources

About