2001
DOI: 10.1016/s0043-1354(00)00260-8
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Inactivation of Cryptosporidium parvum oocysts with ozone and monochloramine at low temperature

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Cited by 71 publications
(44 citation statements)
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“…An interesting strategy proposed relatively recently to improve chemical disinfection is the use of sequential disinfection (Corona-Vasquez et aL 2002). It has been suggested that the application of ozone followed by free chlorine, hypochlorous acid, or chloramines, significantly increases the inactivation rate of Cryptosporidium parvum oocysts (Driedger et al , 2001Rennecker et al 2000). The high efficiency demonstrated by sequential disinfection using the ozone-chlorine pair could be related to the generation of different reactive oxygen species (i.e.…”
Section: Introductionmentioning
confidence: 99%
“…An interesting strategy proposed relatively recently to improve chemical disinfection is the use of sequential disinfection (Corona-Vasquez et aL 2002). It has been suggested that the application of ozone followed by free chlorine, hypochlorous acid, or chloramines, significantly increases the inactivation rate of Cryptosporidium parvum oocysts (Driedger et al , 2001Rennecker et al 2000). The high efficiency demonstrated by sequential disinfection using the ozone-chlorine pair could be related to the generation of different reactive oxygen species (i.e.…”
Section: Introductionmentioning
confidence: 99%
“…In this study, we used excystation with this pre-incubation procedure as a proxy measure of viability. Other researchers have also successfully used excystation to determine viability of ozonated oocysts (Rennecker et al 1999;Driedger et al 2001). Although DAPI was not used to indicate viability in this experiment, excystation can improve the permeability of oocysts to DAPI staining, enhancing visualisation of the sporozoites and nuclei in addition to the observation of excysted oocysts using DIC microscopy (Campbell et al 1992).…”
Section: Discussionmentioning
confidence: 96%
“…Although pasteurization kills Cryptosporidium oocysts ( 12 ), no data exist on the use of ozonation against Cryptosporidium in food or juice products, where turbidity and low temperature render ozonation less effective ( 13 , 14 ). Furthermore, ozonation is difficult to standardize because effectiveness depends on contact time and concentration.…”
Section: Discussionmentioning
confidence: 99%