Inactivation of bacterial opportunistic skin pathogens by nonthermal DC-operated afterglow atmospheric plasma

Heller, Loree C.; Edelblute, Chelsea; Mattson, A.M.; Hao, Xiaolong; Kolb, Juergen F.

doi:10.1111/j.1472-765x.2011.03186.x

Cited by 30 publications

(22 citation statements)

References 51 publications

(98 reference statements)

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“…Furthermore, the effect of acidification might be deferred. This might explain why, in our and other studies, the treatment of tissue samples, in vivo or ex vivo, results in decontamination efficiencies that are two to three orders of magnitude lower than that for agar cultures for the same exposure times [99], [120]. Accordingly, the study of the inactivation of microorganisms on skin is a topic which requires further study.…”

Section: Inactivation Agentsmentioning

confidence: 44%

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Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Kolb

Mattson

Edelblute

et al. 2012

IEEE Trans. Plasma Sci.

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We evaluated a nonthermal plasma jet for a respective use to prevent infections from bacteria and yeasts. The plasma jet is generated from the flow of ambient air with 8 slm through a microhollow cathode discharge assembly that is operated with a direct current of 30 mA. With these parameters, the temperature in the jet reaches 43 • C at 10 mm from the discharge. Agar plates that were inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, and Candida kefyr were treated at this distance, moving the plates through the jet in a meander that covered a 2 cm by 2 cm area. Different exposure times were realized by changing the speed of the movement and adjusting the distance between consecutive passes. S. aureus was most responsive to the exposure with a reduction in the number of colony forming units of 5.5 log steps in 40 s. All other microorganisms show a more gradual inactivation with exposure times. For all bacteria, a clearing of the treated area is achieved in about 2.5-3.5 min, corresponding to log-reduction factors of 5.5-6.5. Complete inactivation of the yeast requires about 7 min. Both S. aureus and C. kefyr show considerable inactivation also outside the immediate treatment area, while P. aeruginosa and A. baumannii do not. We conclude that differences in the morphologies of the membrane structures are responsible for the diverging results, together with a targeted response to different agents provided with the plasma jet. For the gram negative bacteria, we hold short-lived agents, acting across a short range, responsible, while for the other microorganisms, longer lived species seem more important. Our measurements show that neither heat, ultraviolet radiation, nor the generation of ozone can be responsible for the observed results. The most prominent long lived reaction product found is nitric oxide, which, by itself or through induced chemical reactions, might affect cell viability.

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Section: Inactivation Agentsmentioning

confidence: 44%

“…Likewise, we kept to the microhollow cathode discharge geometry as described earlier. These conditions were also maintained for the study of effects on skin, both in vivo and ex vivo [49], [120]. The collected data constitute the basis of a discussion of possible mechanisms and of our future research.…”

Section: Resultsmentioning

confidence: 99%

Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Kolb

Mattson

Edelblute

et al. 2012

IEEE Trans. Plasma Sci.

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“…The concentrations for the most relevant long‐living species that have been generated were quantified depending on different operating conditions. The results are relevant in comparison with recent investigations on the inactivation of different microorganisms and allow for a better understanding of inactivation mechanisms with this particular treatment method and for predictions of operating parameters for more efficient treatments.…”

Section: Introductionmentioning

confidence: 96%

“…As a result, non‐thermal plasma jets are considered a promising method for the decontamination and treatment of sensitive surfaces as, for example, found in wound care . Different jet‐configurations have been presented and have demonstrated the inactivation of microorganisms . Most of these devices are operated with noble gases and the discharge is sustained by an oscillating excitation scheme (rf or microwave voltages) or pulsed high voltages.…”

Section: Introductionmentioning

confidence: 99%

Nitric Oxide Generation with an Air Operated Non‐Thermal Plasma Jet and Associated Microbial Inactivation Mechanisms

Hao¹,

Mattson

Edelblute

et al. 2014

Plasma Processes & Polymers

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Chemical species that are generated by a plasma jet in a microhollow cathode discharge geometry when operated with air and a dc voltage were investigated. Nitric oxide (NO) is found as the dominant long-lived reaction product with concentrations of several hundreds of ppm. In comparison, the concentrations observed for nitric dioxide (NO 2 ) and ozone (O 3 ) are negligible. The concentrations of NO are increasing with increasing electric power but decreasing with increasing flow rates. Simultaneously, NO 2 concentrations are increasing slightly. The results suggest that the observed far reaching biocidal effect of the plasma jet depends on the generation of nitric oxide.

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“…[8]–[10]. Two major mechanisms are involved in the inactivation of microorganisms by plasma treatment: direct destruction by UV irradiation of microorganisms [11], and erosion of the microorganisms by oxygen atoms or radicals emanating from the plasma itself [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of Seed Treatment by Cold Plasma on the Resistance of Tomato to Ralstonia solanacearum (Bacterial Wilt)

et al. 2014

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This study investigated the effect of cold plasma seed treatment on tomato bacterial wilt, caused by Ralstonia solanacearum (R. solanacearum), and the regulation of resistance mechanisms. The effect of cold plasma of 80W on seed germination, plant growth, nutrient uptake, disease severity, hydrogen peroxide (H2O2) concentration and activities of peroxidase (POD; EC 1.11.1.7), polyphenol oxidase (PPO; EC 1.10.3.2) and phenylalanine ammonia lyase (PAL; EC 4.3.1.5) were examined in tomato plants. Plasma treatment increased tomato resistance to R. solanacearum with an efficacy of 25.0%. Plasma treatment significantly increased both germination and plant growth in comparison with the control treatment, and plasma-treated plants absorbed more calcium and boron than the controls. In addition, H2O2 levels in treated plants rose faster and reached a higher peak, at 2.579 µM gFW−1, 140% greater than that of the control. Activities of POD (421.3 U gFW−1), PPO (508.8 U gFW−1) and PAL (707.3 U gFW−1) were also greater in the treated plants than in the controls (103.0 U gFW−1, 166.0 U gFW−1 and 309.4 U gFW−1, respectively). These results suggest that plasma treatment affects the regulation of plant growth, H2O2 concentration, and POD, PPO and PAL activity in tomato, resulting in an improved resistance to R. solanacearum. Consequently, cold plasma seed treatment has the potential to control tomato bacterial wilt caused by R. solanacearum.

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Inactivation of bacterial opportunistic skin pathogens by nonthermal DC-operated afterglow atmospheric plasma

Cited by 30 publications

References 51 publications

Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Nitric Oxide Generation with an Air Operated Non‐Thermal Plasma Jet and Associated Microbial Inactivation Mechanisms

Effect of Seed Treatment by Cold Plasma on the Resistance of Tomato to Ralstonia solanacearum (Bacterial Wilt)

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